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2020 ◽  
Author(s):  
Zhenguang Zhao ◽  
Norman Metanis

Highly valuable bioconjugated molecules must be synthesized through efficient, chemoselective chemical modifications of peptides and proteins. Herein we report the chemoselective modification of peptides and proteins via a reaction between selenocysteine residues and aryl/alkyl radicals. <i>In situ </i>radical generation from hydrazine substrates and copper ions proceeds rapidly in neat aqueous buffer at near neutral pH (5-8), providing a variety of Se-modified linear and cyclic peptides and proteins conjugated to aryl and alkyl molecules, as well as to affinity label tag (biotin). This chemistry opens a new avenue for chemical protein modifications.


2020 ◽  
Author(s):  
Zhenguang Zhao ◽  
Norman Metanis

Highly valuable bioconjugated molecules must be synthesized through efficient, chemoselective chemical modifications of peptides and proteins. Herein we report the chemoselective modification of peptides and proteins via a reaction between selenocysteine residues and aryl/alkyl radicals. <i>In situ </i>radical generation from hydrazine substrates and copper ions proceeds rapidly in neat aqueous buffer at near neutral pH (5-8), providing a variety of Se-modified linear and cyclic peptides and proteins conjugated to aryl and alkyl molecules, as well as to affinity label tag (biotin). This chemistry opens a new avenue for chemical protein modifications.


ChemBioChem ◽  
2020 ◽  
Vol 21 (14) ◽  
pp. 2049-2059
Author(s):  
Zhou Sha ◽  
Sujatha Chilakala ◽  
George Crabill ◽  
Iteen Cheng ◽  
Yan Xu ◽  
...  

Biochemistry ◽  
2019 ◽  
Vol 58 (25) ◽  
pp. 2834-2843 ◽  
Author(s):  
Pei W. Thomas ◽  
Michael Cammarata ◽  
Jennifer S. Brodbelt ◽  
Arthur F. Monzingo ◽  
R. F. Pratt ◽  
...  
Keyword(s):  

Tetrahedron ◽  
2018 ◽  
Vol 74 (26) ◽  
pp. 3273-3277 ◽  
Author(s):  
Chia-Fu Chang ◽  
Adelphe Mfuh ◽  
Jinxu Gao ◽  
Hung-Yi Wu ◽  
Christina M. Woo

2013 ◽  
Vol 21 (12) ◽  
pp. 3597-3601 ◽  
Author(s):  
James D. Vasta ◽  
Joshua J. Higgin ◽  
Elizabeth A. Kersteen ◽  
Ronald T. Raines
Keyword(s):  

2011 ◽  
Vol 337 (1-2) ◽  
pp. 96-100 ◽  
Author(s):  
W. Edward Visser ◽  
Alies A.A. van Mullem ◽  
Jurgen Jansen ◽  
Theo J. Visser

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