Diazaborines Oxidize Slowly with H2O2 but Rapidly with Peroxynitrite in Aqueous Buffer

Reactive oxygen species (ROS) such as H2O2 and peroxynitrite (ONOO-) oxidize arylboronic acids to their corresponding phenols. When used in molecular imaging probes and in ROS-responsive molecules, however, simple arylboronic...

Lipidomic and in-gel analysis of maleic acid co-polymer nanodiscs reveals differences in composition of solubilized membranes

Membrane proteins are key in a large number of physiological and pathological processes. Their study often involves a prior detergent solubilization step, which strips away the membrane and can jeopardize membrane protein integrity. A recent alternative to detergents encompasses maleic acid based copolymers (xMAs), which disrupt the lipid bilayer and form lipid protein nanodiscs (xMALPs) soluble in aqueous buffer. Although xMALPs are often referred to as native nanodiscs, little is known about the resemblance of their lipid and protein content to the native bilayer. Here we have analyzed prokaryotic and eukaryotic xMALPs using lipidomics and in-gel analysis. Our results show that the xMALPs content varies with the chemical properties of the used xMA.

Highly sensitive and ratiometric luminescence sensing of heparin through templated cyanostilbene assemblies

Heparin templated highly emissive cluster formation of di-cationic cyanostilbenes was utilized for the fluorometric detection of the polyanion in aqueous buffer, human serum and plasma.

A Colorimetric Sensor Array for the Classification of Biologically Relevant Tri-, Di- and Mono-phosphates

We describe here a supramolecular sensing system for the array-based classification of biologically relevant mono-, di-, and tri-phosphates in aqueous buffer. The system is based on a colorimetric indicator displacement...

Selective ion transport across a lipid bilayer in a protic ionic liquid

A tethered lipid bilayer assembled in an ionic liquid (IL) preserves selective ion transport by valinomycin and retains its structure upon exchange with aqueous buffer, paving the way for complex, non-aqueous bio-based assemblies.