Engineering Electrospun Nanofibers for the Treatment of Oral Diseases

With the increase of consumption of high-sugar foods, beverages, tobacco, and alcohol, the incidence rate of oral diseases has been increasing year by year. Statistics showed that the prevalence of oral diseases such as dental caries, dental pulpal disease, and periodontal disease has reached as high as 97% in 2015 in China. It is thus urgent to develop functional materials or products for the treatment of oral diseases. Electrospinning has been a widely used technology that is capable of utilizing polymer solution to generate micro/nano fibers under an appropriate high voltage condition. Owing to their excellent structures and biological performances, materials prepared by electrospinning technology have been used for a wide range of oral-related applications, such as tissue restoration, controlled drug release, anti-cancer, etc. In this regard, this article reviews the application and progress of electrospun nanofibers to various oral diseases in recent years. Firstly, engineering strategies of a variety of nanofiber structures together with their resultant functions will be introduced. Then, biological functions of electrospun nanofibers as well as their applications in the treatment of oral diseases are summarized and demonstrated. Finally, the development viewpoint of functional nanofibers is prospected, which is expected to lay the foundation and propose the direction for further clinical application.

From Inner Topological Structure to Functional Nanofibers: Theoretical Analysis and Experimental Verification

The mechanical strength of spider silk is the highest among all natural fibers, and its flexibility is also excellent; this phenomenon can be explained geometrically, due to its hierarchical structure, the last cascade of which beginning with well-ordered macromolecules. The inner topological structure of a nanofiber plays an important role in controlling its functions, e.g., its mechanical, electrical and chemical properties. This paper shows that nanoparticles can be well-ordered in the electrospinning process as a result, the nanofibers’ properties can be adjusted. Some experiments are designed to verify our theoretical prediction.

New Patent on Electrospinning for Increasing Rutin Loading in Nanofibers

Background: The electrospinning and the bubble electrospinning provide facile ways for the fabrication of functional nanofibers by incorporating rutin/hydroxypropyl-β-cyclodextrin inclusion complex (RT/HP-β-CD-IC) in Polyvinyl Alcohol (PVA). Few patents on incorporation of rutin and cyclodextrin in nanofibers has been reported. Objective: The study aimed at increasing the loading amount of rutin in the electrospun nanofibers to obtain ultraviolet resistant property. Methods: Rutin was encapsulated in the cavity of RT/HP-β-CD and formed an inclusion complex. Fourier Transform Infrared Spectroscopy (FTIR) and Differential Scanning Calorimeter (DSC) was used to verify the formation of inclusion complexes. Results: The results showed that the inclusion between rutin and HP-β-CD had been successfully formed. The surface morphologies of nanofibrous membranes were characterized by Scanning Electron Microscope (SEM), which indicated that adding RT/HP-β-CD inclusion complexes had little influence on the morphologies and diameters of the fibers. Ultraviolet resistant results also confirmed the inclusion complex had increased the loading amount in the final nanofibrous mats, and thus had good ultraviolet resistant properties. Conclusion: The formed inclusion complexes had obviously enhanced the loading amount of rutin in electrospun PVA nanofibers, indicating that encapsulation of rutin in the cavity of HP-β-CD is a good way to increase the loading amount.

Two Functional Nanofibers of Polyaniline with Application in Supercapacitor and Photosensor

A series of nanosheets and nanofibers polyaniline was prepared via controlled polymerization. The optimum acid doping amount of polyaniline was obtained (acetic acid:aniline = 7:9; hydrochloric acid:aniline = 2.5:5; citric acid:aniline = 3:5), in which the specific capacitance can reach 162.5 F/g, 271.25 F/g, 240.99 F/g, respectively. The C–V curve indicating the nanorods polyaniline photosensitive sensor chip exhibits excellent sensitivity to green light and blue light. The photocurrent of the sample doped with hydrochloric acid is closest to the 10–6 A. It can be concluded that the PANI has an immense potential in photosensitive sensors and supercapacitors.

Fabrication of Antibacterial Nanofibrous Membrane Infused with Essential Oil Extracted from Tea Tree for Packaging Applications

Nanofibers made by electrospinning are being applied to an unlimited number of applications. In this paper, we propose the fabrication of antimicrobial functional nanofibers infused with essential oil for packaging applications that can extend the shelf-life of fruits. The morphology of nanofibers with different concentrations of essential oil was characterized by SEM and mechanical enhancement was confirmed via universal testing machine (UTM). The surface chemistry and crystalline of the nanofibers were investigated by FTIR and XRD, respectively. The CO2 reduction study was carried out using a hand-made experimental apparatus and nanofiber hydrophobicity, which can prevent moisture penetration from the outside, was evaluated by contact angle. Antimicrobial properties of the functional nanofibers were estimated by using Gram-negative/positive bacteria. The cytotoxicity of the functional nanofibers was studied using fibroblast cells. Furthermore, this study investigated how long the shelf-life of tomatoes was extended. The nanofibers could serve as a multifunctional packaging, as an emerging technology in agricultural products, and even contribute to a better quality of various distributed agricultural products.

Using Self-Assembling Peptides to Integrate Biomolecules into Functional Supramolecular Biomaterials

Throughout nature, self-assembly gives rise to functional supramolecular biomaterials that can perform complex tasks with extraordinary efficiency and specificity. Inspired by these examples, self-assembly is increasingly used to fabricate synthetic supramolecular biomaterials for diverse applications in biomedicine and biotechnology. Peptides are particularly attractive as building blocks for these materials because they are based on naturally derived amino acids that are biocompatible and biodegradable; they can be synthesized using scalable and cost-effective methods, and their sequence can be tailored to encode formation of diverse architectures. To endow synthetic supramolecular biomaterials with functional capabilities, it is now commonplace to conjugate self-assembling building blocks to molecules having a desired functional property, such as selective recognition of a cell surface receptor or soluble protein, antigenicity, or enzymatic activity. This review surveys recent advances in using self-assembling peptides as handles to incorporate biologically active molecules into supramolecular biomaterials. Particular emphasis is placed on examples of functional nanofibers, nanovesicles, and other nano-scale structures that are fabricated by linking self-assembling peptides to proteins and carbohydrates. Collectively, this review highlights the enormous potential of these approaches to create supramolecular biomaterials with sophisticated functional capabilities that can be finely tuned to meet the needs of downstream applications.