Cardiac-Autonomic and Hemodynamic Responses to a Hypertonic, Sugar-Sweetened Sports Beverage in Physically Active Men

Hydration practices may confound heart rate variability (HRV) measurements when collected in the pre-training period. We aimed to determine the effects of ingesting a hypertonic, sugar-sweetened sports beverage on HRV and hemodynamic parameters in physically active young men. Fifteen subjects consumed 591 ml of Gatorade (6% carbohydrate, ~330 mOsmol/kg), 591 ml water, or 10 ml water (control) in random order on separate days following overnight fasting. HRV and hemodynamics were evaluated in 5-min windows immediately before (T1) and 5-10 min (T2), 25-30 min (T3), 40-45 min (T4), and 55-60 min (T5) post-drinking. Root-mean square of successive differences and the standard deviation of normal RR intervals increased post-water intake at all time-points relative to T1 (P <0.05). No increases were observed post-Gatorade intake, though small effect sizes (ES) were noted at T2 and T3 (P >0.05, ES = 0.27 - 0.32). Systemic vascular resistance increased at T2 post-Gatorade intake and at T2 and T3 post-water intake (P <0.05). No interactions were observed for blood pressure measures, stroke volume, or cardiac output. Gatorade does not evoke cardiovascular adjustments to the same magnitude as water. Practitioners should wait at least 45 min to record HRV post-Gatorade intake and >60 min post-water intake. Key Findings: ● Equal volumes of cold water and Gatorade produce inequivalent cardiac-autonomic and hemodynamic responses. ● HRV responses of greater amplitude and duration were observed following intake of water versus Gatorade. ● Failure to account for recent fluid intake may result in misinterpretation of autonomic status.

Effects of Two-Week Betaine Supplementation on Apoptosis, Oxidative Stress, and Aerobic Capacity after Exhaustive Endurance Exercise

This study evaluated the effects of 2 weeks of betaine supplementation on apoptosis, oxidative stress, and aerobic capacity after exhaustive endurance exercise (EEE). A double-blind, crossover, and counterbalanced design was adopted, with 10 healthy male participants asked to consume betaine (1.25 g of betaine mixed with 300 mL of sports beverage, twice per day for 2 weeks) or placebo (300 mL of sports beverage). All participants performed a graded exercise test on a treadmill to determine the maximal oxygen consumption (VO2max) before supplementation and then performed the EEE test at an intensity of 80% VO2max after 2 weeks of supplementation. The time to exhaustion, peak oxygen consumption, maximal heart rate, and average heart rate were recorded during the EEE test. Venous blood samples were drawn before, immediately after, and 3 h after the EEE test to assess apoptosis and the mitochondrial transmembrane potential (MTP) decline of lymphocytes as well as the concentrations of thiobarbituric acid reactive substance and protein carbonyl. The results indicated that lymphocyte apoptosis was significantly higher immediately after and 3 h after EEE than before exercise in participants in the placebo trial. However, lymphocyte apoptosis exhibited no significant differences among the three time points in participants in the betaine trial. Moreover, apoptosis in the betaine trial was significantly lower immediately after and 3 h after exercise compared with the placebo trial. No differences were noted for other variables. Thus, 2 weeks of betaine supplementation can effectively attenuate lymphocyte apoptosis, which is elevated by EEE. However, betaine supplementation exhibited no effects on MTP decline, oxidative stress, or aerobic capacity.

THE INFLUENCE OF AN AMARANTH-BASED BEVERAGE ON CYCLING PERFORMANCE: A PILOT STUDY

The present study aimed to evaluate the effectiveness of an amaranth-based beverage (CHO-P) on cycling performance and hydration status, despite containing a total caloric content higher than that of a commercial sports beverage (CHO-P: 52.48 kcal per 100 mL vs CHO: 24 kcal per 100 mL). In a randomized, crossover design, six cyclists performed two exercise tests separated by seven days. Each test comprised two time-trials (32.20 km and 5 km) separated by 10 min of rest. Participants consumed either an amaranth-based beverage (CHO-P; 10% and 1.5% concentrations) or a commercial sports beverage (CHO; 6%). Changes in hematocrit and body mass, ratings of perceived exertion, and average power were assessed throughout both tests. 32.2-km time-trial performance was enhanced with CHO-P compared to CHO (54.3 ± 4.1 min vs 55.6 ± 4.8 min; p<0.05). However, no other variablemeasured in this study was significantly different between beverage types. Further laboratory based research should be performed to further explore the ergogenic potential of amaranth supplementation during endurance exercise.

The ingestion of protein with a maltodextrin and fructose beverage on substrate utilisation and exercise performance

The study investigated the ingestion of maltodextrin, fructose, and protein on exogenous carbohydrate oxidation (CHOEXO) and exercise performance. Seven trained cyclists and (or) triathletes (maximal oxygen consumption, 59.20 ± 9.00 mL·kg−1·min−1) performed 3 exercise trials that consisted of 150 min of cycling at 50% maximal power output (160 ± 11 W), followed by a 60-km time trial. One of 3 beverages were randomly assigned during each trial and consumed at 15-min intervals: (i) 0.84 g·min−1 maltodextrin + 0.52 g·min−1 fructose + 0.34 g·min−1 protein (MD+F+P); (ii) 1.10 g·min−1 maltodextrin + 0.60 g·min−1 fructose (MD+F); or (iii) 1.70 g·min−1 maltodextrin (MD). CHOEXO and fuel utilisation were assessed via measurement of expired air 13C content and indirect calorimetry, respectively. Mean total CHO oxidation (CHOTOT) rates were 2.35 ± 0.18, 2.76 ± 0.08, and 2.61 ± 0.17 g·min−1 with MD, MD+F, and MD+F+P, respectively, although not significantly different. Peak CHOEXO rates with MD+F were significantly greater by 41.4% (p = 0.001) and 45.4% (p = 0.0001) compared with MD+F+P and MD, respectively (1.57 ± 0.22 g·min−1, 1.11 ± 0.08 g·min−1, and 1.08 ± 0.11 g·min−1, respectively). Performance times were 2.2% and 5.0% faster with MD+F compared with MD+F+P and MD, respectively; however, they were not statistically significant. Ingestion of an MD−fructose−protein commercial sports beverage significantly reduced peak and mean CHOEXO rates compared with MD+F, but did not significantly influence CHOTOT. The addition of protein to an MD+F beverage did not enhance performance times.

The Effect of Ice Slushy Ingestion and Mouthwash on Thermoregulation and Endurance Performance in the Heat

Purpose:The purpose of this study was to establish whether sensory factors associated with cold-beverage ingestion exert an ergogenic effect on endurance performance independent of thermoregulatory or cardiovascular factors.Methods:Ten males performed three trials involving 90 min of steady state cycling (SS; 62% VO2max) in the heat (32.1 ± 0.9 °C, 40 ± 2.4% relative humidity) followed by a 4 kJ/kg body mass time trial (TT). During SS, participants consumed an identical volume (260 ± 38g) of sports beverage (7.4% carbohydrate) every 15 min as either ice slushy (–1 °C; ICE), thermoneutral liquid (37 °C; CON), or thermoneutral liquid consumption with expectorated ice slushy mouthwash (WASH).Results:Rectal temperature, hydration status, heart rate, and skin blood flow were not different between trials. Gastrointestinal (pill) temperature was lower in ICE (35.6 ± 2.7 °C) versus CON (37.4 ± 0.7 °C, p = .05). Heat storage tended to be lower with ICE during SS (14.7 ± 8.4W.m−2, p = .08) and higher during TT (68.9 ± 38.6W.m−2, p = .03) compared with CON (22.1 ± 6.6 and 31.4 ± 27.6W.m−2). ICE tended to lower the rating of perceived exertion (RPE, 12.9 ± 0.6, p = .05) and improve thermal comfort (TC, 4.5 ± 0.2; p = .01) vs. CON (13.8 ± 1.0 and 5.2 ± 0.2 respectively). WASH RPE (13.0 ± 0.8) and TC (4.8 ± 0.2) tended to be lower versus CON (p = .07 and p = .09 respectively). ICE improved performance (18:28 ± 1:03) compared with CON (20:24 ± 1:46) but not WASH (19:45 ± 1:43).Conclusion:Improved performance with ICE ingestion likely resulted from the creation of a gastrointestinal heat sink, reducing SS heat storage. Although the benefits of cold-beverage consumption are more potent when there is ingestion, improved RPE, TC, and meaningful performance improvement with WASH supports an independent sensory effect of presenting a cold stimulus to the mouth.