retrograde response
Recently Published Documents


TOTAL DOCUMENTS

36
(FIVE YEARS 2)

H-INDEX

16
(FIVE YEARS 0)

2021 ◽  
Author(s):  
Elissa Tjahjono ◽  
Alexey V. Revtovich ◽  
Natalia V. Kirienko

AbstractMonitoring of mitochondrial functions is crucial for organismal survival. This task is performed by mitochondrial surveillance or quality control pathways, which are activated by signals originating from mitochondria and relayed to the nucleus (retrograde response) to start the transcription of protective genes. In Caenorhabditis elegans, several systems exist, including the UPRmt, MAPKmt, and the ESRE pathway. These pathways are highly conserved and their loss results in compromised survival following mitochondrial stress.In this study, we found a novel interaction between the box C/D snoRNA core proteins (snoRNPs) and mitochondrial surveillance and innate immunity pathways. We showed that C/D snoRNPs are required for the full expressions of UPRmt and ESRE upon stress. Meanwhile, we found that the loss of C/D snoRNPs increased immune responses. Understanding the “molecular switch” mechanisms of interplay between these pathways may be important for understanding of multifactorial processes, including response to infection or aging.


2020 ◽  
Vol 6 (51) ◽  
pp. eabc9955 ◽  
Author(s):  
Radha Desai ◽  
Daniel A. East ◽  
Liana Hardy ◽  
Danilo Faccenda ◽  
Manuel Rigon ◽  
...  

Mitochondria drive cellular adaptation to stress by retro-communicating with the nucleus. This process is known as mitochondrial retrograde response (MRR) and is induced by mitochondrial dysfunction. MRR results in the nuclear stabilization of prosurvival transcription factors such as the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB). Here, we demonstrate that MRR is facilitated by contact sites between mitochondria and the nucleus. The translocator protein (TSPO) by preventing the mitophagy-mediated segregation o mitochonria is required for this interaction. The complex formed by TSPO with the protein kinase A (PKA), via the A-kinase anchoring protein acyl-CoA binding domain containing 3 (ACBD3), established the tethering. The latter allows for cholesterol redistribution of cholesterol in the nucleus to sustain the prosurvival response by blocking NF-κB deacetylation. This work proposes a previously unidentified paradigm in MRR: the formation of contact sites between mitochondria and nucleus to aid communication.


2019 ◽  
Author(s):  
Oxana Radetskaya ◽  
Rebecca K. Lane ◽  
Troy Friedman ◽  
Aria Garrett ◽  
Michael Nguyen ◽  
...  

ABSTRACTThe p38 mitogen-activated protein kinase (MAPK) PMK-3 controls a life-extending retrograde response in the nematode Caenorhabditis elegans that is activated following mitochondrial electron transport chain (ETC) disruption and is distinct from known longevity-promoting pathways. Here we show that the long isoform of PMK-3 expressed exclusively in the gut, rather than neurons, is sufficient to fully extend the life of animals exposed to mild ETC dysfunction. Surprisingly, constitutive activation of PMK-3 using a gain-of-function MAP3K/DLK-1 mutant does not extend the life of wild-type worms due to dampening of the DLK-1/PMK-3 signaling axis with age. We further show that core components of the ESCRT-III machinery, including ISTR-1, CHMP2B (CC01A4.2) and RAB-11.1, are required for life extension following ETC disruption. ESCRT proteins are needed for extracellular vesicle (EV) formation, lysosomal traffic and other functions requiring membrane encapsulation away from the cytoplasm. Together, our findings underscore PMK-3 as a pivotal factor controlling life extension in worms following mitochondrial ETC disruption and illustrate the importance of the endomembrane system to this process. Our findings raise the possibility that EVs may act as intra-organismal signaling vehicles to control aging.


2019 ◽  
Author(s):  
Swati Sharma

This manuscript has been withdrawn by the author as it was submitted and made public without the full consent of all the authors. Therefore, the author does not wish this work to be cited as reference for the project. If you have any questions, please contact the corresponding author.


2019 ◽  
Author(s):  
Radha Desai ◽  
Daniel A. East ◽  
Liana Hardy ◽  
James Crosby ◽  
Manuel Rigon ◽  
...  

2018 ◽  
Author(s):  
Radha Desai ◽  
Daniel A East ◽  
Liana Hardy ◽  
James Crosby ◽  
Manuel Rigon ◽  
...  

AbstractMitochondria drive cellular adaptation to stress by retro-communicating with the nucleus. This process is known as Mitochondrial Retrograde Response (MRR) and is induced by mitochondrial dysfunctions which perturb cell signalling. MRR results in the nuclear stabilization and activation of pro-survival transcription factors such as the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB). Here we demonstrate that MRR is facilitated by the formation of contact sites between mitochondria and the nucleus which establish microdomains of communication between the two organelles. The 18kD Translocator Protein (TSPO), which de-ubiquitylates and stabilizes the mitochondrial network preventing its mitophagy-mediated segregation, is required for this interaction. The tethering TSPO enacts is mediated by the complex formed with the Protein Kinase A via the A-kinase anchoring protein Acyl-CoA Binding Domain Containing 3 (ACBD3) and allows the redistribution of cholesterol which sustains the pro-survival response by blocking NF-kB de-acetylation. This work proposes a new paradigm in the mitochondrial retro-communication by revealing the existence of contact sites between mitochondrial and the nucleus and a signalling role for cholesterol.


Genetics ◽  
2016 ◽  
Vol 204 (2) ◽  
pp. 659-673 ◽  
Author(s):  
J. C. Jiang ◽  
S. W. Stumpferl ◽  
A. Tiwari ◽  
Q. Qin ◽  
J. F. Rodriguez-Quinones ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document