Photodynamic effectiveness of laser diode combined with ozone to reduce STaphylicoccus aureus biofilm with exogenous chlorophyll of Dracaena angustifolia leaves

Photodynamic inactivation is an effective treatment that uses light irradiation, photosensitizer and oxygen. The aim of this study was to determine photodynamic effectiveness of laser diode combined with ozone to reduce Staphylococcus aureus biofilm using exogenous chlorophyll (Chlo). The chlorophyll was extracted from leave of Dracaena angustifolia. To determine the antibacterial effect of S. aureus biofilm treatments, samples were separated into Chlo, Laser, Chlo+Laser, Ozone, Ozone+Laser, Chlo+Ozone+Laser categories. The data were analyzed using ANOVA test. The result of this study showed that Chlo+Ozone+Laser combine treatment at 20 s exposure of ozone with 4 min of irradiation time lead to 80.26 % reduction of biofilm activity, which was the highest efficacy of all the treatment groups. The combination of laser, chlorophyll and lower ozone concentration increases the effectiveness of photodynamic inactivation.

Fotophoresis and laser treatment in patients with chroniceczema

The aim. To evaluate the efficiency of physiotherapeutic methods and combine therapy on immune system and on oxidation process in patientes with chronic eczema. Materials and methods. There were 32 patients with severe chronic eczema, who were treated with standard systemic and topical therapy plus photophoresis with mometasone furoat (1 mg) and supracutaneus laser irradiation of blood (1 procedure). The efficiency of therapy was detected by the changes of EASI index, cell and humoral immunity parameters, antioxidation status. Results. After using of combine therapy in severe eczema patients positive changes in severity of cutaneous process and prolongation of remission periods were found out as well as normalization of immune status and levels of oxidation processes. Conclusion. The combine treatment of patients with severe chronic eczema is effective, available method of therapy of chronic eczema patients.

SOSTOYaNIE KOSTNOY TKANI I RISK RAZVITIYa OSTEOPOROZA U ZhENShchIN V STADII POLNOY REMISSIIBOLEZNI ITsENKO-KUShINGA NA FONE FIZIOLOGIChESKOYMENOPAUZY

It is well known that clinical signs of endogenous hypercorticism including secondary osteoporosis rapidly reverse on the base of hormonal remission after radiosurgical treatment of Cushing's disease (CD). The aim of the study was to assess BMD and bone turnover biochemical markers in postmenopausal women with complete CD-remission. We examined 43 women aged 38-67 years in physiological postmenopause who had a complete long-term hormonal CD-remission for at least 2 years (11±6,8 years in average) after combine treatment with hemiadrenalectomy and radiosurgery. Control group comprised 98 healthy postmenopausal women at the same age and postmenopausal age. Our main findings were BMD in postmenopausal CD-patients was higher vs. controls in lumbar spine (1,233±0,17 vs. 1,146±0,20 g/cm2, p