Effect of different protein based feed on the growth of mahseer

For the proper growth of fish, it is necessary to feed the fish with a proper and balanced diet. A study was conducted to find out the effect of different protein-based diets on fingerlings of Tor putitora (mahseer). A feed with dietary protein levels of 35%, 40%, 45%, and 50% were prepared. The effect of different protein-based feed on weight gain, standard growth rate (SGR), food conversion ratio (FCR), percent weight gain, food conversion efficiency (FCE), and protein efficiency ratio (PER) was studied. An increase was observed in the growth rate with an increase in protein concentration up to 45%. The fingerlings fed a 45% protein diet shown the highest growth, followed by 50%, 40%, and 35%. The SGR value was greatest for 45% protein diet (8.56) followed by 50% and 40%, while the least values were observed for 35% protein feed (1.57). The same trend was observed for FCE. The highest PER values was observed in fishes fed 45% protein-based feed (0.65) followed by 50% (0.56), 40% (0.38) and35% (0.17). The food conversion ratio was lowest for 45% protein diet (3.41), while the greatest for 35% protein feed (16.85). It was concluded that a 45% protein-based diet was the best feed formulation for higher production of Tor putitora. However, research on the same percentage of protein diet is recommended for yearlings.

Rising Temperature Effects on Growth and Gastric Emptying Time of Freshwater African Catfish (Clarias Gariepinus) Fingerlings

The present study was carried out to analyse the effect of water temperature on two components: (1) growth performance, and (2) gastric emptying time (GET) of African catfish Clarias gariepinus fingerlings. After 70 days, it was observed that experimental temperatures had no significant effects on the growth performance parameters, except for food conversion ratio (FCR) and food conversion efficiency (FCE). GET observation through X-radiography denoted that the shortest GET (10 h) was observed in fish reared at 32 °C and the longest GET (16 h) was observed in fish reared at 26 °C. The rapid digestion rate coincides with the FCR and FCE obtained in this study. Considering the limited scope of our study, more extensive studies on the impact of water temperature on other fish physiological parameters should be pursued. A better understanding of this research topic would be beneficial for the growth of African catfish fingerling aquaculture.

The Use of Temperate Tannin Containing Forage Legumes to Improve Sustainability in Forage–Livestock Production

Greenhouse gas emissions from ruminant livestock production systems contribute significantly to the environmental footprint of agriculture. Emissions are lower for feedlot systems than for grass-based systems primarily because of the extra time required for grass-finished cattle to reach slaughter weight. In contrast, legume forages are of greater quality than grasses, which enhances intake and food conversion efficiencies, leading to improvements in production and reductions in environmental impacts compared with forage grasses. In addition, the presence of certain bioactives in legumes such as condensed tannins (CT) enhance the efficiency of energy and protein use in ruminants relative to grasses and other feeds and forages. Grazing tannin-containing legumes also reduce the incidence of bloat and improve meat quality. Synergies among nutrients and bioactives when animals graze diverse legume pastures have the potential to enhance these benefits. Thus, a diversity of legumes in feeding systems may lead to more economically, environmentally, and socially sustainable beef production than grass monocultures or feedlot rations.

Aquaponic Biofloc Technology by Swamp Bacteria Probiotic for Clarias Catfish Rearing

Conventional catfish culture in Ogan Ilir (OI) have not optimal production, it can be increased by biofloc and aquaponics with local swamp bacteria as a starter. The purpose of this study was to apply biofloc technology and a combination of aquaponic-biofloc to increase the productivity of catfish (Clarias sp.) rearing. The study used two ponds, a biofloc circular pond and a combined biofloc and aquaponic (biofloqua) for two months. The results of the specific growth rate of fish in the aquaponic biofloc system were higher than that of the biofloc system alone. The survival of the fish during two rearing months of the Biofloqua system was 100% while the Biofloc system was 92-96%. The water quality data that measured were temperature, 30.3–31.9oC, Dissolved oxygen 4.5–7.2 mg.l-1, pH 6-7, and ammonia concentration 0.27-0.71 mg.l-1 in the biofloc system and 29.5-31.3oC, 4.7-7.4 mg.l-1, pH 6-7, 0.20-0.53 mg.l-1 in biofloqua system, respectively. The floc volume formation rate in the biofloc system alone reached 1.1 ml.l-1 per day, which was above the floc formation rate for the biofloqua system 0.42 ml.l-1 per day, due to the use of nutrients for plant growth. The combination of biofloc and aquaponics showed more optimal growth performance and both were efficient in feed (Food Conversion Ratio / FCR <1.00). Although the results of the 5% level t test showed no difference in the performance of the aquaculture biota between the two systems, Biofloqua can be an alternative to increase fish farmer income by harvesting vegetables and fish together.

Substitution of fishmeal with solid-state fermented pigeon pea and effects on growth and gut microbiomes of Nile Tilapia

Solid state fermented pigeon pea meal (FPP) was used in substituting fish meal (FM) in diets of Oreochromis niloticus. Five diets varying in inclusion levels of FM: FPP as followed: Feed 1, 250:50; Feed 2, 200:100; Feed 3, 150:150; Feed 4, 100:200 and Feed 5, 50:250 were made. A commercial feed (Feed 6), was used as a control. Triplicate groups of tilapia fingerling were stocked in plastic aquaria at six fish per unit and fed ad libitum for 60days. Results showed that specific growth rate (SGR) of tilapia fed Feed 5 (2.60±0.12% day-1) was better (p<0.05) than those recorded in Feed 1 (2.36±.07 % day-1). Similarly, food conversion ratio (FCR) (1.13±0.01) and mean weight gain (60.00±0.04g) were higher (p<0.05) in Feed 5 when compared with those in Feed 1 where FCR was 1.19±0.02 and MWG (50.96±0.04g). The results of Aspartate amino transferase (AST) and alanine amino transferase (ALT) decreased with increasing inclusion level of FPP. Gut microbial load increased with increasing level of FPP. The fish group fed Feed 5 had the highest foregut (2.60 x 10-5 CFU/ml), midgut (3.98 x 10-5 CFU/ml) and hindgut (4.52 x 10-5 CFU/ml) micrbiota. In general, gut microbiomes were dominated by cellulose and carbohydrate utilizing bacteria: Citrobacter fruendi, Bacillus subtilis and Staphylococcus aureus. Inference from the study revealed that O. niloticus was able to utilize the diets due to the presence of probiotics in them.

Efek Paparan Subletal Limbah Cair Industri Penyamakan Kulit terhadap Rasio Konversi Pakan dan Laju Pertumbuhan Ikan Nila (Oreochromis niloticus)

ABSTRACT Wastewater from the tanning industry of UPTD XX has entered the waters of Batang Anai River, West Sumatra. This wastewater quality exceeds the established quality standards and can be harmful to tilapia as a river biota. This study aimed to analyze the effect of sublethal tannery wastewater on food conversion ratio (FCR) and the specific growth rate (SGR) of tilapia. This study was conducted in three conditions: zero wastewater exposure (control), 1.85%, and 3.69% wastewater exposure. Each situation was set in triplo and observed for 28 days. We analyzed the correlation between duration exposure to the FCR and SGR by using regression and correlation analysis. Results showed an increase in the FCR value during observation in both wastewater exposure variations of 1.85% and 3.69%, with FCR values consecutively 1.19 and 1.75. At the same time, the control experiment showed a decreasing value of FCR. On the contrary, SGR values were decreased during observation in both wastewater exposure variations of 1.85% and 3.69%, consecutively 3.09% and 3.72%. While increasing SGR value was observed in the control experiment. A reliable correlation was obtained between the FCR and SGR ratio of tilapia to the exposure duration (r = 0.99). Furthermore, multivariate analysis showed a significant difference between the FCR and SGR to the variation and period of direction. It can be concluded that the longer the exposure time and the higher the concentration of exposure, decreasing the food uptake of tilapia and reducing the specific growth rate. Keywords: tilapia, ratio growth rate, tannery wastewater, feed conversion, West Sumatra ABSTRAK Limbah cair dari industri penyamakan UPTD XX, Sumatera Barat telah memasuki perairan Sungai Batang Anai, Sumatra Barat. Kualitas air limbah ini melebihi standar kualitas yang ditetapkan dan dapat berbahaya bagi nila sebagai salah satu biota sungai. Penelitian ini bertujuan untuk menganalisis pengaruh air limbah penyamakan kulit terhadap Rasio Konversi Pakan (Food Conversion Ratio/FCR) dan Laju Pertumbuhan Spesifik (Specific Growth Rate/SGR) pada ikan nila. Penelitian ini dilakukan dalam tiga variasi, yakni tanpa paparan air limbah (uji kontrol), paparan air limbah 1,85% dan 3,69%. Setiap variasi dilakukan secara triplo dan diamati selama 28 hari. Korelasi antara lama paparan dengan rasio FCR serta SGR, dianalisis dengan menggunakan regresi dan analisis korelasi. Selanjutnya analisis multivariat menggunakan ANOVA two-way untuk melihat perbedaan signifikan FCR dan SGR terhadap variasi dan durasi paparan. Hasil penelitian menunjukkan peningkatan nilai FCR di kedua variasi paparan air limbah 1,85% dan 3,69% dengan nilai FCR berturut-turut 1,19 dan 1,75. FCR dalam uji kontrol menunjukkan penurunan nilai FCR. Sebaliknya, nilai SGR cenderung menurun selama pengamatan pada kedua variasi paparan air limbah 1,85% dan 3,69%, dengan nilai SGR berturut-turut 3,09% dan 3,72%, sementara peningkatan nilai SGR terjadi pada uji kontrol. korelasi yang sangat kuat diperoleh dari nilai FCR dan SGR terhadap durasi paparan (r = 0,99). Selanjutnya, uji signifikansi ANOVA menunjukkan perbedaan yang signifikan antara rasio FCR dan SGR dengan variasi dan durasi paparan air limbah. Dapat disimpulkan bahwa semakin lama waktu paparan dan semakin tinggi konsentrasi paparan air limbah, mengurangi serapan pakan ikan nila dan mengurangi tingkat pertumbuhan. Kata kunci: ikan nila, laju pertumbuhan, limbah cair penyamakan kulit, rasio konversi pakan, Sumatra Barat

DOES K-DIFORMATE SUPPLEMENTATION IMPROVES GROWTH AND DIET STAIBLITY OF JUVENILE WHITE SHRIMP(LITOPENAEUS VANNAMEI)?

A study was conducted to investigate the effects of potassium diformate (K-diformate) supplementation in two protein levels on growth, food conversion and survival in juvenile white shrimp, litoppenaeusvannamei.Two dietary protein levels, 41% dietary protein(CP41) and 37% dietary protein(CP37) was formulated and supplemented of graded K-diformate levels 0%, 0.1%, 0.2%, 0.4%, 0.8%, 1.4% and 2.0%.Post larvae white shrimp (Litopenaeusvannamei) of initial mean weight 0.82±0.08g were randomly stocked in 45 aquarium tanks with a working volume of 50 L (60×40×30 cm ) at a stocking density of 10 shrimps per aquarium.The result of this study did not show significant difference in percentage weight gain and survival rate among shrimps fed CP41 and CP37 with graded K-diformate supplemented diet. No K-diformate dose response was observed at the end of the study. The results of present study proof that K-diformate supplemented diet do not affect water pH and feeding diet stability in water.

Phenol-rich fulvic acid as a water additive enhances growth, reduces stress, and stimulates the immune system of fish in aquaculture

Aquaculture has become imperative to cover the demands for dietary animal protein. Simultaneously, it has to overcome prejudices from excessive use of antibiotics and environmental impacts. Natural supplements are traditionally applied orally. In this study, we demonstrated another pathway: the gills. Humic substances are immunostimulants and a natural part of every aquatic ecosystem, making them ideal to be used as bath stimulants. Five and 50 mg C/L of a fulvic acid-rich humic substance was added for 28 days to the water of juvenile rainbow trout (Oncorhynchus mykiss). This fulvic acid is characterized by a high content of phenolic moieties with persistent free radicals and a high electron exchange capacity. The high concentration of the fulvic acid significantly increased growth and reduced the food conversion ratio and the response to a handling-stressor. Phagocytosis and potential killing activity of head kidney leukocytes were increased, as well as the total oxyradical scavenging capacity (TOSC) and lysozyme activity in the gills. In conclusion, immunostimulation via gills is possible with our fulvic acid, and the high phenolic content improved overall health and stress resistance of fish.