The quiescent centre and root apical meristem: organization and function

This special issue is dedicated to the 100th anniversary of the birth of Frederick Albert Lionel Clowes, who discovered the quiescent centre (QC) of the root apical meristem (RAM). His discovery was a foundation for contemporary studies of the QC and RAM function, maintenance, and organization. RAM function is fundamental for cell production and root growth. This special issue bundles reviews on the main tendencies, hypotheses, and future directions, and identifies unknowns in the field.

Three-dimensional quantitative analysis of the Arabidopsis quiescent centre

Quiescent centre (QC) cells represent an integral part of the root stem cell niche. They typically display a low division frequency that has been reported to be controlled by hormone signaling and different regulators, including the ETHYLENE RESPONSE FACTOR 115 (ERF115) transcription factor and D-type cyclins. Here, we applied a three-dimensional (3D) imaging to visualize the Arabidopsis QC cell number, volume and division patterns, including visualization of anticlinal divisions that cannot be deduced from longitudinal 2D imaging. We found that 5-day-old seedlings possess on average eight QC cells which are organized in a monolayered disc. In a period of 7 d, half of the QC cells undergo anticlinal division in a largely invariant space. Ectopic expression of ERF115 and CYCLIN D1;1 (CYCD1;1) promote both anticlinal and periclinal QC cell divisions, the latter resulting in a dual-layered QC zone holding up to 2-fold more QC cells compared with the wild type. In contrast, application of cytokinin or ethylene results in an increase in the number of periclinal, but a decrease in anticlinal QC divisions, suggesting that they control the orientation of QC cell division. Our data illustrate the power of 3D visualization in revealing unexpected QC characteristics.

The quiescent centre of the root apical meristem: Conceptual developments from Clowes to modern times

In this work we discuss the concepts of the quiescent centre (QC) of the root apical meristem (RAM) and their change over time, from their formulation by F.A.L. Clowes to the present. This review is dedicated to the 100 th anniversary of the birth of Clowes, and we present his short biography and full bibliography of Clowes’ work. Over time, the concept of the QC proved to be useful for the understanding of RAM organization and behaviour. We focus specifically on conceptual developments, from the organization of the QC to understanding its functions in RAM maintenance and activity, ranging from a model species, Arabidopsis thaliana, to crops. Concepts of initial cells, stem cells, and heterogeneity of the QC cells in context of functional and structural stem cells are considered. We review the role of the QC in the context of cell flux in the RAM and the nature of quiescence of the QC cells. We discuss the origin of the QC and fluctuation of its size in ontogenesis and why the QC cells are more resistant to stress. Contemporary concepts of organizer and stem cell niche are also considered. We also propose how the stem cell niche in the RAM can be defined in roots of a non-model species.