A Low-Cost Contactless Overhead Micrometer Surface Scanner

The design and implementation of a contactless scanner and its software are proposed. The scanner regards the photographic digitization of planar and approximately planar surfaces and is proposed as a cost-efficient alternative to off-the-shelf solutions. The result is 19.8 Kppi micrometer scans, in the service of several applications. Accurate surface mosaics are obtained based on a novel image acquisition and image registration approach that actively seeks registration cues by acquiring auxiliary images and fusing proprioceptive data in correspondence and registration tasks. The device and operating software are explained, provided as an open prototype, and evaluated qualitatively and quantitatively.

Ambiguus Tiles: Origami Rhombic Pyramid Tiles for Creating Dual-View Tile Mosaics

This article presents a novel expressive medium called Ambiguus tiles, which construct dual-view tile mosaics. While similar to wall and surface mosaics made with conventional tiles, Ambiguus tiles can be assembled and rearranged to create dual-view tile mosaics in any size and form. Fact vs. Fiction, a letter sculpture composed of 115 Ambiguus tiles, is presented here as an example of the creative possi-bilities for using Ambiguus tiles as a ready-to-use design material. As seen in Fact vs. Fiction, Ambiguus tiles are particularly effective in communicating social messages.

Profiles of Surface Mosaics on Chronic Lymphocytic Leukemias Distinguish Stable and Progressive Subtypes

Purpose. Chronic lymphocytic leukemia (CLL) is a heterogeneous disease, some patients may survive for many years, while 20-30% of patients progress and may die within several years. Currently, there is not a single procedure that enables accurate prognosis and triaging of those patients who need immediate and aggressive treatment. All CLL cells are characterised by the expression of the B-cell antigens CD19, CD20, CD21, CD22 and CD23, with aberrant expression of the T-cell antigen CD5. Methods. We have developed a CD antibody microarray (DotScan) containing 182 immobilised CD antibodies that has been used to obtain extensive surface profiles of CLL cells obtained from 96 patients. Results. Of these 182 antigens, 27 were significantly differentially expressed between stable, stable-progressive and progressive CLL. Some of these antigens are not expressed on normal B-cells and may be targets for therapeutic antibodies against CLL. Unsupervised hierarchical clustering of the surface profiles from 96 patients showed that those with progressive CLL could be distinguished based solely upon this ‘disease signature’. The sensitivity (proportion of actual positives correctly identified) was 67.9%, the specificity (proportion of negatives correctly identified) was 77.5%, and the accuracy was 71.9%. Conclusions. Considerable effort by a number of research groups has resulted in identification of individual markers for progressive CLL, but their collective use is yet to provide a test that identifies CLL patients at risk. Data presented here provide a basis for development of a simple test using an antibody microarray. This article is open to POST-PUBLICATION REVIEW. Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page.