scholarly journals Modulation of Skin Inflammatory Response by Active Components of Silymarin

Molecules ◽  
2018 ◽  
Vol 24 (1) ◽  
pp. 123 ◽  
Author(s):  
Jana Juráňová ◽  
Juliette Aury-Landas ◽  
Karim Boumediene ◽  
Catherine Baugé ◽  
David Biedermann ◽  
...  
Keyword(s):  
Wound Healing ◽  
Prolonged Exposure ◽  
Dermal Fibroblasts ◽  
Enzyme Linked Immunosorbent Assay ◽  
Activator Protein 1 ◽  
Human Dermal Fibroblasts ◽  
Active Components ◽  
Mobility Shift ◽  
Anti Inflammatory

In this study, we compared selected silymarin components, such as quercetin (QE), 2,3-dehydrosilybin (DHS) and silybin (SB), with the anti-inflammatory drug indomethacin (IND) in terms of their wound healing potential. In view of the fact that pathological cutaneous wound healing is associated with persistent inflammation, we studied their anti-inflammatory activity against inflammation induced by bacterial lipopolysaccharide (LPS). We investigated the regulation of crucial pro-inflammatory transcription factors—nuclear factor kappa-B (NF-κB) and activator protein 1 (AP-1)—as well as the expression of downstream inflammatory targets by Western blotting, real-time PCR (RT-PCR), electrophoretic mobility shift assay (EMSA), and/or enzyme-linked immunosorbent assay (ELISA) in vitro using primary normal human dermal fibroblasts (NHDF). We demonstrated the greater ability of DHS to modulate the pro-inflammatory cytokines production via the NF-κB and AP-1 signaling pathways when compared to other tested substances. The prolonged exposure of LPS-challenged human dermal fibroblasts to DHS had both beneficial and detrimental consequences. DHS diminished interleukin-6 (IL-6) and interleukin-8 (IL-8) secretion but induced the significant upregulation of IL-8 mRNA associated with NF-κB and AP-1 activation. The observed conflicting results may compromise the main expected benefit, which is the acceleration of the healing of the wound via a diminished inflammation.

scholarly journals Aristolochia bracteolata Enhances Wound Healing in vitro through Anti-inflammatory and Proliferative Effect on Human Dermal Fibroblasts and Keratinocytes

2017 ◽  
Vol 9 (6s) ◽  
pp. s129-s136 ◽  
Author(s):  
Dinesh Murugan Girija ◽  
Mangathayaru Kalachaveedu ◽  
Rajasekaran Subbarayan ◽  
Preethi Jenifer ◽  
Suresh Ranga Rao
Keyword(s):  
Wound Healing ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Proliferative Effect ◽  
Anti Inflammatory ◽  
Aristolochia Bracteolata

In vitro Proliferation and Wound Healing Effects of Narcissus tazetta L. Bulb on Primary Human Dermal Fibroblasts

2018 ◽  
Vol 20 (6) ◽  
pp. 1-11 ◽  
Author(s):  
Maryam Rameshk ◽  
Fariba Sharififar ◽  
Mitra Mehrabani ◽  
Abbas Pardakhty ◽  
Alireza Farsinejad
Keyword(s):  
Wound Healing ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Narcissus Tazetta ◽  
In Vitro Proliferation

scholarly journals Interaction of Gold Nanorods with Human Dermal Fibroblasts: Cytotoxicity, Cellular Uptake, and Wound Healing

Nanomaterials ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 1131
Author(s):  
Nouf N. Mahmoud ◽  
Lubna M. Al-Kharabsheh ◽  
Enam A. Khalil ◽  
Rana Abu-Dahab
Keyword(s):  
Wound Healing ◽  
Cellular Uptake ◽  
Gold Nanorods ◽  
Healing Rate ◽  
Scratch Test ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Poly Ethylene ◽  
Wound Healing Rate

Herein, the cytotoxicity, cellular uptake and wound healing of human dermal fibroblasts were investigated upon treatment with gold nanorods (GNR) decorated with different ligands. Neutral and cationic poly ethylene glycol (PEG)-decorated GNR demonstrated the least cytotoxicity and cellular internalization, while anionic- and bovine serum albumin (BSA)-coated GNR revealed significant cytotoxicity and cellular uptake into human dermal fibroblasts. The cell scratch test demonstrated that neutral, cationic PEGylated GNR and anionic-decorated GNR have accelerated the wound healing rate in vitro after 24 h of incubation with scratched human dermal fibroblasts compared to control, while there was a drastic retardation of wound healing rate of scratched fibroblasts upon exposure to BSA-GNR accompanied with a significant release of the inflammatory cytokine; interlukin-1β (IL-1β). The cytotoxicity of GNR against the dermal cells and their ability to enhance the wound healing in vitro are greatly linked to their surface modifications.

Wound Healing Activity of Carbonized Rice Husk

2012 ◽  
Vol 602-604 ◽  
pp. 1196-1199
Author(s):  
Nuraly Akimbekov ◽  
Zulhair A. Mansurov ◽  
J. Jandosov ◽  
Ilya E. Digel ◽  
Mathias Gossmann ◽  
...  
Keyword(s):  
Wound Healing ◽  
Rice Husk ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Rat Skin ◽  
Cellular Behavior ◽  
Proliferation In Vitro ◽  
Wound Healing Activity

The carbonized rice husk (CRH) was evaluated for its wound healing activity in rats using excision models. In this study, the influences of CRH on wound healing in rat skin in vivo and cellular behavior of human dermal fibroblasts in vitro were investigated. The obtained results showed that the CRH treatment promoted wound epithelization in rats and exhibited moderate inhibition of cell proliferation in vitro. CRH with lanolin oil treated wounds were found to epithelize faster as compared to controls.

scholarly journals Dieckol, an Algae-Derived Phenolic Compound, Suppresses UVB-Induced Skin Damage in Human Dermal Fibroblasts and Its Underlying Mechanisms

Antioxidants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 352
Author(s):  
Lei Wang ◽  
Jun-Geon Je ◽  
Hye-Won Yang ◽  
You-Jin Jeon ◽  
Seungheon Lee
Keyword(s):  
Brown Seaweed ◽  
Dermal Fibroblasts ◽  
Mitogen Activated Protein Kinase ◽  
Activator Protein 1 ◽  
Human Dermal Fibroblasts ◽  
Skin Damage ◽  
Mitogen Activated Protein ◽  
Underlying Mechanisms ◽  
Hdf Cells

Ultraviolet (UV) irradiation is considered to be the primary environmental factor that causes skin damage. In the present study, we investigated the protective effect of dieckol (DK), a compound isolated from the brown seaweed Ecklonia cava, against UVB-induced skin damage in human dermal fibroblasts (HDF cells). The results indicated that DK effectively inhibited the activity of collagenase. DK remarkably reduced the intracellular reactive oxygen species level and improved the viability of UVB-irradiated HDF cells. Besides, DK significantly and dose-dependently improved collagen synthesis and inhibited intracellular collagenase activity in UVB-irradiated HDF cells. In addition, DK markedly reduced the expression of proinflammatory cytokines and matrix metalloproteinases. Further analyses revealed that these processes were mediated through the regulation of nuclear factor kappa B, activator protein 1, and mitogen-activated protein kinase signaling pathways in the UVB-irradiated HDF cells. In conclusion, these results indicate that DK possesses strong in vitro photoprotective effects and therefore has the potential to be used as an ingredient in the cosmeceutical industry.

scholarly journals Effect of Bioactive Phytochemicals from Phlomis viscosa Poiret on Wound Healing

Plants ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 609 ◽  
Author(s):  
Ludmila Yarmolinsky ◽  
Arie Budovsky ◽  
Leonid Yarmolinsky ◽  
Boris Khalfin ◽  
Vladimir Glukhman ◽  
...  
Keyword(s):  
Wound Healing ◽  
Beneficial Effect ◽  
Chronic Wounds ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
In Vivo Models ◽  
Bioactive Phytochemicals ◽  
Pro Inflammatory Cytokines

Phlomis viscosa Poiret is an evergreen shrub growing in Israel, Turkey, Lebanon, and Syria with acknowledged pro-wound healing (WH) properties. In this study, we evaluated the pro-WH potential of selected compounds found in this plant. Among the pro-WH compounds (identified by us) was a combination of three chemicals—diosmin, 1-octen-3-ol, and himachala-2,4-diene which enhanced WH significantly both in in vitro and in vivo models. The determined phytochemicals combination could be used for the treatment of chronic wounds. The effect of the extracts, diosmin, 1-octen-3-ol on the secretion of pro-inflammatory cytokines, IL-6 (A) and IL-8 (B) by human dermal fibroblasts was significant (p < 0.001). In addition, the beneficial effect of extracts of P. viscosa and its phytochemicals on WH was evidenced by inhibiting the growth of several WH delaying microorganisms.

scholarly journals 1,2-Bis[(3-Methoxyphenyl)Methyl]Ethane-1,2-Dicarboxylic Acid Reduces UVB-Induced Photodamage In Vitro and In Vivo

Antioxidants ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 452 ◽  
Author(s):  
Wu ◽  
Lin ◽  
Hou ◽  
Chang ◽  
Wen ◽  
...  
Keyword(s):  
Protein Expression ◽  
Dicarboxylic Acid ◽  
Map Kinases ◽  
Mouse Skin ◽  
Dermal Fibroblasts ◽  
Premature Aging ◽  
Activator Protein 1 ◽  
Anti Inflammatory

This study investigated the effects and mechanisms of 1,2-bis[(3-methoxyphenyl)methyl]ethane-1,2-dicarboxylic acid (S4), a sesamin derivative, on anti-inflammation and antiphotoaging in vitro and in vivo. Human skin fibroblasts were treated with S4 and did not show cytotoxicity under concentrations of 5–50 µM. In addition, S4 also reduced ultraviolet (UV)B-induced intracellular reactive oxygen species (ROS) production. Additionally, S4 inhibited UVB-induced phosphorylation of mitogen-activated protein (MAP) kinases, activator protein-1 (AP-1), and matrix metalloproteinases (MMPs) overexpression. Furthermore, S4 also inhibited UVB-induced Smad7 protein expression and elevated total collagen content in human dermal fibroblasts. For anti-inflammatory activity, S4 inhibited UVB-induced nitric oxide synthase (i-NOS) and cyclooxygenase (COX)-2 protein expression and inhibited nuclear factor-kappaB (NF-ĸB) translocation into the nucleus. S4 ameliorated UVB-induced erythema and wrinkle formation in hairless mice. On histological observation, S4 also ameliorated UVB-induced epidermal hyperplasia and collagen degradation. S4 reduced UVB-induced MMP-1, interleukin (IL)-6, and NF-ĸB expression in the mouse skin. The results indicated that S4 had antiphotoaging and anti-inflammatory activities, protecting skin from premature aging.

scholarly journals Lupeol, a Pentacyclic Triterpene, Promotes Migration, Wound Closure, and Contractile Effect In Vitro: Possible Involvement of PI3K/Akt and p38/ERK/MAPK Pathways

Molecules ◽  
2018 ◽  
Vol 23 (11) ◽  
pp. 2819 ◽  
Author(s):  
Fernando Pereira Beserra ◽  
Meilang Xue ◽  
Gabriela Maia ◽  
Ariane Leite Rozza ◽  
Cláudia Helena Pellizzon ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Wound Healing ◽  
Therapeutic Potential ◽  
Collagen Gel ◽  
Skin Wound ◽  
Dermal Fibroblasts ◽  
Pentacyclic Triterpene ◽  
Skin Wound Healing ◽  
Anti Inflammatory

Skin wound healing is a dynamic and complex process involving several mediators at the cellular and molecular levels. Lupeol, a phytoconstituent belonging to the triterpenes class, is found in several fruit plants and medicinal plants that have been the object of study in the treatment of various diseases, including skin wounds. Various medicinal properties of lupeol have been reported in the literature, including anti-inflammatory, antioxidant, anti-diabetic, and anti-mutagenic effects. We investigated the effects of lupeol (0.1, 1, 10, and 20 μg/mL) on in vitro wound healing assays and signaling mechanisms in human neonatal foreskin keratinocytes and fibroblasts. Results showed that, at high concentrations, Lupeol reduced cell proliferation of both keratinocytes and fibroblasts, but increased in vitro wound healing in keratinocytes and promoted the contraction of dermal fibroblasts in the collagen gel matrix. This triterpene positively regulated matrix metalloproteinase (MMP)-2 and inhibited the NF-κB expression in keratinocytes, suggesting an anti-inflammatory effect. Lupeol also modulated the expression of keratin 16 according to the concentration tested. Additionally, in keratinocytes, lupeol treatment resulted in the activation of Akt, p38, and Tie-2, which are signaling proteins involved in cell proliferation and migration, angiogenesis, and tissue repair. These findings suggest that lupeol has therapeutic potential for accelerating wound healing.

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