Detection, Identification, and Antimicrobial Susceptibility of Campylobacter spp. and Salmonella spp. from Free-Ranging Nonhuman Primates in Sri Lanka

2019 ◽  
Vol 55 (4) ◽  
pp. 879 ◽  
Author(s):  
Cecilia Tegner ◽  
N. P. Sunil-Chandra ◽  
W. R. P. L. I. Wijesooriya ◽  
B. Vijitha Perera ◽  
Ingrid Hansson ◽  
...  
Keyword(s):  
Sri Lanka ◽  
Antimicrobial Susceptibility ◽  
Nonhuman Primates ◽  
Salmonella Spp ◽  
Free Ranging ◽  
Campylobacter Spp

scholarly journals Isolates, Antimicrobial Susceptibility Profiles and Multidrug Resistance of Bacteria Cultured from Pig Submissions in New Zealand

Animals ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1427
Author(s):  
Christopher B. Riley ◽  
Kirsty L. Chidgey ◽  
Janis P. Bridges ◽  
Emma Gordon ◽  
Kevin E. Lawrence
Keyword(s):  
Multidrug Resistance ◽  
New Zealand ◽  
Antimicrobial Susceptibility ◽  
Pasteurella Multocida ◽  
Coagulase Negative Staphylococcus ◽  
Sample Type ◽  
Bacterial Isolates ◽  
Salmonella Spp ◽  
Susceptibility Data ◽  
Campylobacter Spp

Data on the scope of bacterial pathogens present and the frequency of antimicrobial resistance (AMR) in New Zealand’s pigs are limited. This study describes bacterial isolates, antimicrobial susceptibility data, and multidrug resistance (MDR; resistance to ≥3 antimicrobial classes) from New Zealand pig submissions. Porcine test data from June 2003 to February 2016 were obtained from commercial veterinary pathology laboratory records. In total, 470/477 unique submissions resulted in bacterial growth, yielding 779 isolates. Sample type was recorded for 360/477 (75.5%); lung (79/360; 21.9%), faecal (61/360; 16.9%) and intestinal (45/360; 12.5%) were most common. The most common isolates were Escherichia coli (186/779, 23.9%), Actinobacillus pleuropneumoniae (43/779; 5.5%), Streptococcus suis (43/779; 5.5%), unidentified Campylobacter spp. (38/779; 4.9%), alpha haemolytic Streptococci (32/779; 4.1%), coagulase negative Staphylococcus spp. (26/779; 3.3%), and Pasteurella multocida (25/779; 3.2%). Susceptibility results were available for 141/779 (18.1%) isolates from 62/470 (13.2%) submissions. Most were susceptible to trimethoprim-sulphonamide (75/81; 92.6%), but fewer were susceptible to penicillin (37/77; 48.1%), tilmicosin (18/43; 41.9%), or tetracyclines (41/114; 36.0%). No susceptibility data were available for Salmonella spp., Campylobacter spp., or Yersinia spp. isolates. MDR was present in 60/141 (42.6%) isolates. More data on sample submission drivers, antimicrobial drug use, and susceptibilities of important porcine bacterial isolates are required to inform guidelines for prudent antimicrobial use, to reduce their prevalence, human transmission, and to minimise AMR and MDR.

scholarly journals Incidence and antimicrobial susceptibility of Salmonella, Listeria, and Campylobacter spp. in raw “souvlaki” marketed in Thessaloniki (Northern Greece)

2018 ◽  
Vol 66 (3) ◽  
pp. 161 ◽  
Author(s):  
N. SOULTOS (Ν. ΣΟΥΛΤΟΣ) ◽  
E. IOSSIFIDOU (Ε. ΙΩΣΗΦΙΔΟΥ) ◽  
E. PSOMAS (Ε. ΨΩΜΑΣ) ◽  
Z. TZIKAS (Ζ. ΤΖΗΚΑΣ) ◽  
T. LAZOU (Θ. ΛΑΖΟΥ)
Keyword(s):  
Antimicrobial Susceptibility ◽  
Thermal Processing ◽  
Antimicrobial Agents ◽  
Meat Product ◽  
Diffusion Method ◽  
Salmonella Spp ◽  
Northern Greece ◽  
Food Borne ◽  
Food Borne Infections ◽  
Campylobacter Spp

Souvlaki is a popular Greek meat product consisting of small chunks or cubes of pork threaded on a small wooden or metal skewer. In the present study, 105 samples of raw pork souvlaki obtained from retail shops in Thessaloniki (Northern Greece) were screened for the incidence of Salmonella, Listeria, and Campylobacter and their susceptibility to various antimicrobial agents; serotyping of the isolates was also performed. Of the samples tested, 1.9% were positive for Salmonella and yielded 3 serovars (S. SaintPaul, S. Fyris and S. Typhimurium); 31.4% proved positive for Listeria spp. with 6.7% yielding L. monocytogenes isolates belonged to molecular serogroups 2 (serotypes 1/2c and 3c) and 4 ( serotypes 4b, 4d, and 4e). Campylobacter spp. were not detected in any of the samples tested. The antimicrobial susceptibility to various antimicrobial agents of 11 Salmonella strains and 7 L. monocytogenes strains was also determined by disc diffusion method. Salmonella spp. were susceptible to a panel of 12 antibiotics but displayed intermediate resistance to tetracycline. L. monocytogenes isolates were resistant to nalidixic acid and ceftriaxone, partly resistant to clindamycin and cefotaxime, but sensitive to all antibiotics commonly used in veterinary and human listeriosis. Our findings indicate that souvlaki could be a potential vehicle of food borne infections due to strains of L. monocytogenes and Salmonella spp. in the case of inadequate thermal processing. In addition, good hygienic practices must be applied to avoid cross-contamination during preparation or handling of the product.

scholarly journals Contamination Status of Salmonella spp., Shigella spp. and Campylobacter spp. in Surface and Groundwater of the Kelani River Basin, Sri Lanka

Water ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 2187
Author(s):  
M.G.Y.L. Mahagamage ◽  
M.V.S.C. Pathirage ◽  
Pathmalal M. Manage
Keyword(s):  
Surface Water ◽  
Sri Lanka ◽  
River Basin ◽  
Total Coliform ◽  
Salmonella Spp ◽  
E Coli ◽  
Sampling Locations ◽  
Shigella Spp ◽  
Kelani River ◽  
Campylobacter Spp

Waterborne diseases are a global problem that causes more than 2.2 million deaths annually. Therefore, the present study was focused on microbiological contamination of both ground and surface water by means of total coliform, Escherichia coli (E. coli), Salmonella spp., Shigella spp. and Campylobacter spp. Seventy two groundwater and 45 surface water sampling locations were selected to collect water from the head, transitional and meandering regions of the Kelani River Basin for a period of one year (both dry and wet seasons). The results of the study revealed that the entire Kelani River basin was contaminated with total coliform and E. coli bacteria and almost all the sampling locations exceed Sri Lanka Standards Institute (SLSI) guideline value given for drinking water (0 CFU/100 mL). Further, in groundwater, 17 locations were positive for Salmonella spp., whereas only 2 locations were positive for Campylobacter spp. In surface water, 26 and three sampling locations were positive for Salmonella spp. and Campylobacter spp., respectively. In this study, 23 different human pathogenic serovars were isolated and the Salmonella enterica serovar Kentucky was identified as the commonest type. Thus, the result of the study revealed that the consumption of raw water from the Kelani River Basin is unsafe and possible to cause gastrointestinal diseases.

scholarly journals Prevalence and Antimicrobial Resistances of Salmonella spp. Isolated from Wild Boars in Liguria Region, Italy

Pathogens ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 568
Author(s):  
Elisabetta Razzuoli ◽  
Valeria Listorti ◽  
Isabella Martini ◽  
Laura Migone ◽  
Lucia Decastelli ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Wild Boar ◽  
Antimicrobial Susceptibility ◽  
Potential Risk ◽  
Salmonella Enterica ◽  
Intestinal Tract ◽  
Wild Boar Population ◽  
Salmonella Spp ◽  
Wild Boars ◽  
In The Wild

Salmonella spp. is an important zoonotic agent. Wild boars might host this pathogen in the intestinal tract and might represent a risk for Salmonella spp. transmission to humans. Wild boars are widely spread in Liguria, due to the environmental characteristics of the region. The aim of the study was the isolation, typing, and investigation of antimicrobial susceptibility of the isolated strains of Salmonella spp. During the 2013–2017 hunting seasons, 4335 livers of wild boars were collected and analyzed for the presence of Salmonella spp. A total of 260 strains of Salmonella spp. were isolated and characterized, with a prevalence of 6%. The isolated strains belonged to all six Salmonella enterica subspecies. Most of them were identified as Salmonella enterica subs. enterica of which 31 different serotypes were identified. The dominating serotype identified was S. Enteritidis. The antimicrobial resistance profiles of the isolated strains were analyzed against sixteen molecules. Of the isolated strains, 94.6% were resistant to at least one of the tested antimicrobials. This study showed the circulation of resistant Salmonella spp. strains in the wild boar population living in this area of Italy, underling the potential risk for these animals to disseminate this pathogen and its antimicrobial resistances.

scholarly journals Antimicrobial susceptibility testing results from 13 hospitals in Viet Nam: VINARES 2016–2017

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Tien Viet Dung Vu ◽  
◽  
Marc Choisy ◽  
Thi Thuy Nga Do ◽  
Van Minh Hoang Nguyen ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Data Entry ◽  
Sampling Bias ◽  
Antimicrobial Susceptibility Testing ◽  
Viet Nam ◽  
Salmonella Spp ◽  
Invasive Infections ◽  
Shigella Spp ◽  
Analyse Data

Abstract Objective To analyse data from 2016–17 from a hospital-based antimicrobial resistance surveillance with national coverage in a network of hospitals Viet Nam. Methods We analysed data from 13 hospitals, 3 less than the dataset from the 2012–13 period. Identification and antimicrobial susceptibility testing data from the clinical microbiology laboratories from samples sent in for routine diagnostics were used. Clinical and Laboratory Standards Institute 2018 guidelines were used for antimicrobial susceptibility testing interpretation. WHONET was used for data entry, management and analysis. Results 42,553 deduplicated isolates were included in this analysis; including 30,222 (71%) Gram-negative and 12,331 (29%) Gram-positive bacteria. 8,793 (21%) were from ICUs and 7,439 (18%) isolates were from invasive infections. Escherichia coli and Staphylococcus aureus were the most frequently detected species with 9,092 (21%) and 4,833 isolates (11%), respectively; followed by Klebsiella pneumoniae (3,858 isolates – 9.1%) and Acinetobacter baumannii (3,870 isolates – 9%). Bacteria were mainly isolated from sputum (8,798 isolates – 21%), blood (7,118 isolates – 17%) and urine (5,202 isolates – 12%). Among Gram-positives 3,302/4,515 isolates (73%) of S. aureus were MRSA; 99/290 (34%) of Enterococcus faecium were resistant to vancomycin; and 58% (663/1,136) of Streptococcus pneumoniae proportion were reduced susceptible to penicillin. Among Gram-negatives 59% (4,085/6,953) and 40% (1,186/2,958) of E. coli and K. pneumoniae produced ESBL and 29% (376/1,298) and 11% (961/8,830) were resistant to carbapenems, respectively. 79% (2855/3622) and 45% (1,514/3,376) of Acinetobacter spp. and Pseudomonas aeruginosa were carbapenem resistant, respectively. 88% (804/911) of Haemophilus influenzae were ampicillin resistant and 18/253 (7%) of Salmonella spp. and 7/46 (15%) of Shigella spp. were resistant to fluoroquinolones. The number of isolates from which data were submitted in the 2016–2017 period was twice as high as in 2012–2013. AMR proportions were higher in 2016–2017 for most pathogen-antimicrobial combinations of interest including imipenem-resistant A. baumannii, P. aeruginosa and Enterobacterales. Conclusions The data show alarmingly high and increasing resistant proportions among important organisms in Viet Nam. AMR proportions varied across hospital types and should be interpreted with caution because existing sampling bias and missing information on whether isolates were community or hospital acquired. Affordable and scalable ways to adopt a sample- or case-based approach across the network should be explored and clinical data should be integrated to help provide more accurate inferences of the surveillance data.

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