scholarly journals Occurrence of Campylobacter spp., Salmonella spp. and shiga toxin‐producing Escherichia coli in inline milk filters from Swedish dairy farms

2019 ◽  
Vol 40 (1) ◽  
Author(s):  
Catarina Flink ◽  
Karin Nyberg
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Dairy Farms ◽  
Salmonella Spp ◽  
Swedish Dairy ◽  
Campylobacter Spp

Mexican unpasteurised fresh cheeses are contaminated with Salmonella spp., non-O157 Shiga toxin producing Escherichia coli and potential uropathogenic E. coli strains: A public health risk

2016 ◽  
Vol 237 ◽  
pp. 10-16 ◽  
Author(s):  
Rosa Guzman-Hernandez ◽  
Araceli Contreras-Rodriguez ◽  
Rosa Hernandez-Velez ◽  
Iza Perez-Martinez ◽  
Ahide Lopez-Merino ◽  
...  
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Health Risk ◽  
Shiga Toxin ◽  
Salmonella Spp ◽  
Public Health Risk ◽  
E Coli

Microbiological Quality of Retail Imported Unprepared Whole Lettuces: A PHLS Food Working Group Study

1999 ◽  
Vol 62 (4) ◽  
pp. 325-328 ◽  
Author(s):  
C. LITTLE ◽  
D. ROBERTS ◽  
E. YOUNGS ◽  
J. de LOUVOIS
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Microbiological Quality ◽  
Escherichia Coli O157 ◽  
Salmonella Spp ◽  
Shigella Spp ◽  
Natural Microflora ◽  
Production Practices ◽  
Campylobacter Spp

A study of imported unprepared whole lettuces sampled from supermarkets, greengrocers, shops, and market stalls found that all were of acceptable microbiological quality. Twenty-seven out of 151 (18%) imported lettuce samples had Enterobacteriaceae levels of 104 CFU/g or more. However, these bacteria that constitute part of the natural microflora of unprepared vegetables may also be derived from the soil and/or by poor handling. The pathogens, Salmonella spp., Shigella spp., Campylobacter spp., Escherichia coli O157:H7, Vibrio cholerae, Listeria monocytogenes, and also Escherichia coli, an indicator of fecal contamination, were not detected in any imported lettuces, indicating that hygiene, harvesting, and production practices were good. Imported lettuces with Enterobacteriaceae levels of 104 CFU/g or more varied with type of retail premises and the temperature at which the lettuces were displayed. Samples from greengrocers, shops, and market stalls were more likely to contain Enterobacteriaceae at levels in excess of 104 CFU/g than those from supermarkets.

Low Prevalence of Salmonella and Shiga Toxin–Producing Escherichia coli in Lymph Nodes of Australian Beef Cattle

2017 ◽  
Vol 80 (12) ◽  
pp. 2105-2111 ◽  
Author(s):  
Gavin Bailey ◽  
Long Huynh ◽  
Lachlan Govenlock ◽  
David Jordan ◽  
Ian Jenson
Keyword(s):  
Escherichia Coli ◽  
Lymph Nodes ◽  
Shiga Toxin ◽  
Limit Of Detection ◽  
Ground Beef ◽  
Plate Count ◽  
Salmonella Spp ◽  
Live Animal ◽  
E Coli ◽  
Low Prevalence

ABSTRACT Salmonella contamination of ground beef has been viewed as originating from the surface of carcasses. Recent studies have identified lymph nodes as a potential source of Salmonella contamination because these tissues play an active role in containment of pathogens in the live animal and because some lymph nodes are unavoidably present in manufacturing beef trimmings or primal cuts that may be incorporated into ground beef. A survey was conducted of the microbiological status of lymph nodes from Australian cattle at the time of slaughter to determine the prevalence of microbiological contamination. Sets of lymph nodes (n = 197), consisting of the superficial cervical (prescapular), prepectoral, axillary, presternal, popliteal, ischiatic, subiliac (precrural), coxalis, and iliofemoralis (deep inguinal), were collected from five geographically separated Australian abattoirs over a period of 14 months. Samples were tested for the presence of Salmonella spp. and Shiga toxin–producing Escherichia coli by BAX PCR assay. Aerobic plate count, E. coli, and coliforms were enumerated with a lower limit of detection of 80 CFU per node. The observed prevalence of Salmonella within peripheral lymph nodes was 0.48% (7 of 1,464). Two of the seven lymph nodes in which Salmonella organisms were detected came from the same animal. Grass-fed, grain-fed, and cull dairy cattle were all found to have detectable Salmonella in lymph nodes. All Salmonella detections occurred during cooler months of the year. No Shiga toxin–producing E. coli were detected. Aerobic microorganisms were detected above the limit of quantification in 3.2% of nodes (median count 2.24 log per node), and E. coli was detected in 0.8% of nodes (median count 3.05 log per node). The low prevalence of Salmonella and low concentration of aerobic microorganisms in Salmonella-positive lymph nodes of Australian cattle at the time of slaughter suggest that the likelihood of lymph nodes contributing significantly to the presence of Salmonella in ground beef is low.

scholarly journals Occurrence of Campylobacter spp., Salmonella spp. and Escherichia coli in Chicken Carcasses

2017 ◽  
Vol 6 (1) ◽  
pp. 346-355 ◽  
Author(s):  
Isamery A. Machado ◽  
◽  
Adriano S. Okamoto ◽  
José Rafael Modolo ◽  
Noeme S. Rocha ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Salmonella Spp ◽  
Campylobacter Spp

Multiplex PCR method for the detection of human norovirus, Salmonella spp., Shigella spp., and shiga toxin producing Escherichia coli in blackberry, coriander, lettuce and strawberry

2021 ◽  
pp. 103926
Author(s):  
Omar Hernández Hernández ◽  
Ana L. Gutiérrez-Escolano ◽  
Cleo Cancio-Lonches ◽  
Montserrat H. Iturriaga ◽  
Juan Ramiro Pacheco-Aguilar ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multiplex Pcr ◽  
Shiga Toxin ◽  
Salmonella Spp ◽  
Human Norovirus ◽  
Shigella Spp ◽  
Pcr Method

Risk of Escherichia coli O157:H7, Non-O157 Shiga Toxin–Producing Escherichia coli, and Campylobacter spp. in Food Animals and Their Products in Qatar

2015 ◽  
Vol 78 (10) ◽  
pp. 1812-1818 ◽  
Author(s):  
HUSSNI O. MOHAMMED ◽  
KORANA STIPETIC ◽  
AHMED SALEM ◽  
PATRICK McDONOUGH ◽  
YUNG FU CHANG ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Cross Sectional Study ◽  
Campylobacter Coli ◽  
Escherichia Coli O157 ◽  
Cross Sectional ◽  
Fecal Samples ◽  
E Coli ◽  
Cattle Feces ◽  
Campylobacter Spp

Escherichia coli O157:H7, non-O157 E. coli, and Campylobacter spp. are among the top-ranked pathogens that threaten the safety of food supply systems around the world. The associated risks and predisposing factors were investigated in a dynamic animal population using a repeat-cross-sectional study design. Animal and environmental samples were collected from dairy and camel farms, chicken processing plants, and abattoirs and analyzed for the presence of these pathogens using a combination of bacterial enrichment and real-time PCR tests without culture confirmation. Data on putative risk factors were also collected and analyzed. E. coli O157:H7 was detected by PCR at higher levels in sheep and camel feces than in cattle feces (odds ratios [OR], 6.8 and 21.1, respectively). Although the genes indicating E. coli O157:H7 were detected at a relatively higher rate (4.3%) in fecal samples from dairy cattle, they were less common in milk and udder swabs from the same animals (1 and 2%, respectively). Among the food adulterants, E. coli O103 was more common in cattle fecal samples, whereas O26 was more common in sheep feces and O45 in camel feces compared with cattle (OR, 2.6 and 3.1, respectively). The occurrence of E. coli in the targeted populations differed by the type of sample and season of the year. Campylobacter jejuni and Campylobacter coli were more common in sheep and camel feces than in cattle feces. Most of the survey and surveillance of E. coli focused on serogroup O157 as a potential foodborne hazard; however, based on the PCR results, non-O157 Shiga toxin–producing E. coli serotypes appeared to be more common, and efforts should be made to include them in food safety programs.

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