scholarly journals Calotropis gigantea Leaf Extract Increases the Efficacy of 5-Fluorouracil and Decreases the Efficacy of Doxorubicin in Widr Colon Cancer Cell Culture

2018 ◽  
pp. 51-56
Keyword(s):  
Cell Culture ◽  
Colon Cancer ◽  
Cancer Cell ◽  
Leaf Extract ◽  
Colon Cancer Cell ◽  
Calotropis Gigantea

scholarly journals Montmorillonite colloid plates with adsorbed cytochrome c: in vitro cytotoxic effect on colon cancer cell culture

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Svetlana H. Hristova ◽  
Alexandar M. Zhivkov
Keyword(s):  
Cell Culture ◽  
Colon Cancer ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Aqueous Suspension ◽  
Colon Cancer Cell ◽  
Composite Particles ◽  
Total Charge ◽  
Colloid Particles

Abstract Background The apoptosis (a cascade of biochemical reactions leading to suicide of damaged biological cells) is blocked in the cancer cells because of impossibility of cytochrome c (cytC) go out from the mitochondria. However, the apoptosis can be started by introducing of exogenous cytC into cytoplasm using colloid particles as a protein carrier due to ability of the cancer cells to phagocytize extracellular particles with submicron size. Results The clay mineral montmorillonite (MM) were used to prepare aqueous suspension of protein/mineral composite particles by electrostatic adsorption of the positively charged cytC globules on the negatively charged MM colloid plates, and then added to colon cancel culture. The results shows out that separately cytC and MM have no effect but the composite cytC-MM particles kill 95% of the cancer cells after 96 h treatment using equine cytC which is 97% structurally identical with the human cytC. To reach this high cytotoxicity we have formulated requirements to: (a) bare colloid particles (electric charge, form and size), (b) conditions for protein adsorption (concentrations, pH, ionic strength), and (c) suspension with the composite particles (positive total charge and optimal concentration). Due to satisfying these requirements we have reached cytotoxicity which is 1/3 higher than the reached by other authors using different artificial particles. The cytotoxicity rapidly increases with concentration of the cytC-MM particles but further it shows tendency to saturation. Methods The optimal pH 6.5 and the 10:3 mg/mg cytC/MM concentration ratio at adsorption were found out by employing computer (protein electrostatics) and physicochemical methods (microelectrophoresis and colloid electrooptics) to prepare cytC-MM suspension. The anticancer capability of cytC-MM nanoplates were investigated using cell culture of metastasizing colon cancer. Conclusion The in vitro experiments with colon cancer cell culture disclose that cytC-MM composite particles have potential for application in anticancer therapy of superficial neoplasms of the skin and the alimentary system (mouth cavity, esophagus, stomach, jejunum and colon). Graphic abstract

scholarly journals Quantitative Proteomic and Phosphoproteomic Comparison of 2D and 3D Colon Cancer Cell Culture Models

2016 ◽  
Vol 15 (12) ◽  
pp. 4265-4276 ◽  
Author(s):  
Xiaoshan Yue ◽  
Jessica K. Lukowski ◽  
Eric M. Weaver ◽  
Susan B. Skube ◽  
Amanda B. Hummon
Keyword(s):  
Cell Culture ◽  
Colon Cancer ◽  
Cancer Cell ◽  
Colon Cancer Cell ◽  
Culture Models ◽  
2D And 3D ◽  
Cell Culture Models

scholarly journals Multi-Layer Nanofibrous PCL Scaffold-Based Colon Cancer Cell Cultures to Mimic Hypoxic Tumor Microenvironment for Bioassay

Cancers ◽  
2021 ◽  
Vol 13 (14) ◽  
pp. 3550
Author(s):  
Eun-Taex Oh ◽  
Ha Gyeong Kim ◽  
Min-Ho Choi ◽  
Jae-Seon Lee ◽  
Sang Jeong Kim ◽  
...  
Keyword(s):  
Cell Culture ◽  
Colon Cancer ◽  
Tumor Microenvironment ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Cell Cultures ◽  
S Phase ◽  
Colon Cancer Cell ◽  
Colon Cancer Cells ◽  
Hypoxic Tumor

Three-dimensional (3D) cancer cell culture systems have been developed to aid the study of molecular mechanisms in cancer development, identify therapeutic targets, and test drug candidates. In this study, we developed a strategy for mimicking the hypoxic tumor microenvironment in a 3D cancer cell culture system using multi-layer, nanofibrous poly(ε-caprolactone) (PCL) scaffold (pNFS)-based cancer cell cultures. We found that human colon cancer cells infiltrated pNFS within 3 days and could be cultured three-dimensionally within the NFS. When incubated in four stacks of 30 µm-thick pNFS for 3 days, colon cancer cells in layer three showed partially reduced entry into the S phase, whereas those in layer four, located farthest from the media, showed a marked reduction in S-phase entry. As a consequence, cells in layer four exhibited hypoxia-induced disorganization of F-actin on day 3, and those in layers three and four showed an increase in the expression of the hypoxia-induced transcription factor HIF-1α and its target genes, Glut1, CA9, VEGF, and LDHA. Consistent with these results, doxorubicin- and ionizing radiation-induced cell death was reduced in colon cancer cells cultured in layers three and four. These results suggest that pNFS-based multi-layer colon cancer cell cultures mimic the hypoxic tumor microenvironment and are useful for bioassays.

In Vitro Study of Colon Cancer Cell Migration Using E‐Jet 3D Printed Cell Culture Platforms

2018 ◽  
Vol 18 (11) ◽  
pp. 1800205 ◽  
Author(s):  
Juchang Zhong ◽  
Yingjie Zhang ◽  
Jingfei Chen ◽  
Ruiying Huang ◽  
Yikun Yang ◽  
...  
Keyword(s):  
Cell Culture ◽  
Colon Cancer ◽  
Cell Migration ◽  
Cancer Cell ◽  
In Vitro Study ◽  
Colon Cancer Cell ◽  
Vitro Study ◽  
Cancer Cell Migration ◽  
3D Printed
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