scholarly journals Quantitative Proteomic and Phosphoproteomic Comparison of 2D and 3D Colon Cancer Cell Culture Models

2016 ◽  
Vol 15 (12) ◽  
pp. 4265-4276 ◽  
Author(s):  
Xiaoshan Yue ◽  
Jessica K. Lukowski ◽  
Eric M. Weaver ◽  
Susan B. Skube ◽  
Amanda B. Hummon
Keyword(s):  
Cell Culture ◽  
Colon Cancer ◽  
Cancer Cell ◽  
Colon Cancer Cell ◽  
Culture Models ◽  
2D And 3D ◽  
Cell Culture Models

scholarly journals Conjunctival melanoma and electrochemotherapy: preliminary results using 2D and 3D cell culture models in vitro

2018 ◽  
Vol 97 (4) ◽  
pp. e632-e640 ◽  
Author(s):  
Miltiadis Fiorentzis ◽  
Periklis Katopodis ◽  
Helen Kalirai ◽  
Berthold Seitz ◽  
Arne Viestenz ◽  
...  
Keyword(s):  
Cell Culture ◽  
3D Cell Culture ◽  
Conjunctival Melanoma ◽  
Preliminary Results ◽  
Culture Models ◽  
2D And 3D ◽  
Cell Culture Models

Length vs. stiffness: which plays a dominant role in the cellular uptake of fructose-based rod-like micelles by breast cancer cells in 2D and 3D cell culture models?

2018 ◽  
Vol 6 (25) ◽  
pp. 4223-4231 ◽  
Author(s):  
Jiacheng Zhao ◽  
Hongxu Lu ◽  
Yin Yao ◽  
Sylvia Ganda ◽  
Martina H. Stenzel
Keyword(s):  
Breast Cancer ◽  
Cell Culture ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Dominant Role ◽  
3D Cell Culture ◽  
Culture Models ◽  
Nano Rods ◽  
2D And 3D ◽  
Cell Culture Models

Internalization of rod-like micelles by breast cancer cells is significantly affected by the stiffness of nano-rods.

Abstract A59: Assessment of conditional reprogramming to generate 2D and 3D primary human mammary cell culture models

2018 ◽  
Author(s):  
Stacey Chung ◽  
Liting Jin ◽  
Ying Qu ◽  
Liliana J. Gomez ◽  
Bingchen Han ◽  
...  
Keyword(s):  
Cell Culture ◽  
Mammary Cell ◽  
Culture Models ◽  
Mammary Cell Culture ◽  
2D And 3D ◽  
Cell Culture Models

scholarly journals Cellular effects of paclitaxel-loaded iron oxide nanoparticles on breast cancer using different 2D and 3D cell culture models

2018 ◽  
Vol Volume 14 ◽  
pp. 161-180 ◽  
Author(s):  
Stephan Lugert ◽  
Harald Unterweger ◽  
Marina Mühlberger ◽  
Christina Janko ◽  
Sebastian Draack ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Culture ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
3D Cell Culture ◽  
Oxide Nanoparticles ◽  
Cellular Effects ◽  
Culture Models ◽  
2D And 3D ◽  
Cell Culture Models

scholarly journals Montmorillonite colloid plates with adsorbed cytochrome c: in vitro cytotoxic effect on colon cancer cell culture

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Svetlana H. Hristova ◽  
Alexandar M. Zhivkov
Keyword(s):  
Cell Culture ◽  
Colon Cancer ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Aqueous Suspension ◽  
Colon Cancer Cell ◽  
Composite Particles ◽  
Total Charge ◽  
Colloid Particles

Abstract Background The apoptosis (a cascade of biochemical reactions leading to suicide of damaged biological cells) is blocked in the cancer cells because of impossibility of cytochrome c (cytC) go out from the mitochondria. However, the apoptosis can be started by introducing of exogenous cytC into cytoplasm using colloid particles as a protein carrier due to ability of the cancer cells to phagocytize extracellular particles with submicron size. Results The clay mineral montmorillonite (MM) were used to prepare aqueous suspension of protein/mineral composite particles by electrostatic adsorption of the positively charged cytC globules on the negatively charged MM colloid plates, and then added to colon cancel culture. The results shows out that separately cytC and MM have no effect but the composite cytC-MM particles kill 95% of the cancer cells after 96 h treatment using equine cytC which is 97% structurally identical with the human cytC. To reach this high cytotoxicity we have formulated requirements to: (a) bare colloid particles (electric charge, form and size), (b) conditions for protein adsorption (concentrations, pH, ionic strength), and (c) suspension with the composite particles (positive total charge and optimal concentration). Due to satisfying these requirements we have reached cytotoxicity which is 1/3 higher than the reached by other authors using different artificial particles. The cytotoxicity rapidly increases with concentration of the cytC-MM particles but further it shows tendency to saturation. Methods The optimal pH 6.5 and the 10:3 mg/mg cytC/MM concentration ratio at adsorption were found out by employing computer (protein electrostatics) and physicochemical methods (microelectrophoresis and colloid electrooptics) to prepare cytC-MM suspension. The anticancer capability of cytC-MM nanoplates were investigated using cell culture of metastasizing colon cancer. Conclusion The in vitro experiments with colon cancer cell culture disclose that cytC-MM composite particles have potential for application in anticancer therapy of superficial neoplasms of the skin and the alimentary system (mouth cavity, esophagus, stomach, jejunum and colon). Graphic abstract

Abstract 3312: A novel plate-based assay for screening autophagic activity in 2D and 3D cell culture models

2017 ◽  
Author(s):  
Dan F. Lazar ◽  
Amani A. Gillette ◽  
Braeden L. Butler ◽  
Christopher T. Eggers ◽  
Brock F. Binkowski ◽  
...  
Keyword(s):  
Cell Culture ◽  
3D Cell Culture ◽  
Culture Models ◽  
Autophagic Activity ◽  
2D And 3D ◽  
Cell Culture Models
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