Total phenolic and flavonoid content, antioxidant and antimicrobial activity of extracts from Tordylium maximum

2013 ◽  
Author(s):  
Jelena S. Matejic
Keyword(s):  
Antimicrobial Activity ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Antioxidant And Antimicrobial Activity ◽  
Phenolic And Flavonoid

scholarly journals Ramification of pH in pectinase-assisted extraction on the antioxidant capacity of Arabica spent coffee ground (SCG) extract

Food Research ◽  
2020 ◽  
Vol 4 (4) ◽  
pp. 1303-1311
Author(s):  
N.A. Shukri ◽  
Z. Mohd Zin ◽  
N. MohdMaidin ◽  
Hasmadi M. ◽  
M.K. Zainol
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Spent Coffee Ground ◽  
Coffee Ground ◽  
Phenolic And Flavonoid

Spent coffee ground (SCG) is the by-product of coffee processing that is produced up to 45% in coffee beverage and instant coffee processing and is believed to contain high amounts of antioxidants. Despite the fact that SCG has exerted many advantages, the information on obtaining the antioxidant values using enzymes are still scarce. The objective of the study was therefore to determine the total phenolic and flavonoid content of antioxidant activity and antimicrobial activity in Arabica SCG extracted using pectinase at different pH values. Arabica SCG was extracted using pectinase at pH 3, pH 4, pH 5, pH 6 and pH 7 and analyzed for its anti-microbial activity and antioxidant properties (DPPH, FRAP, total phenolic and total flavonoid content along with individual flavonoids using HPLC). Arabica SCG was extracted using pectinase at pH 3, pH 4, pH 5, pH 6 and pH 7 and analyzed for its antimicrobial activity and antioxidant properties (DPPH, FRAP, total phenolic and total flavonoid content along with individual flavonoids using HPLC). The result showed that the antioxidant capacity of the SCG extract at pH 4 exhibited higher DPPH and FRAP values. The total phenolic and flavonoid content exhibited in (1.38±4.42, mg GAE/g sample and 22.57±0.27 mg QE/g of dry sample). Flavonoids namely quercetin, kaempferol, rutin, gallic acid, catechin, epigallocatechin gallate, pcoumaric acid and myricetin were present in all samples at various levels. The SCG in pH 7 extract showed the highest concentrations of the individual flavonoid compound in the sample and the highest inhibition zone on Pseudomonas aeruginosa and Staphylococcus aureus. Thus, the optimum pH of the pectinase which is pH 4-5 had provided the highest yield in antioxidant capacity and activity of the Arabica SCG extract with this extraction method that can be used for food preservation as well as in the nutraceutical industry.

scholarly journals Components responsible for antimicrobial activity of propolis from continental and Mediterranean regions in Croatian

2017 ◽  
Vol 35 (No. 5) ◽  
pp. 376-385 ◽  
Author(s):  
Gajger Ivana Tlak ◽  
Pavlović Iva ◽  
Bojić Mirza ◽  
Kosalec Ivan ◽  
Srečec Siniša ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Therapeutic Potential ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Microdilution Method ◽  
Mediterranean Regions ◽  
Chromatographic Profile ◽  
Popular Subject ◽  
Phenolic And Flavonoid ◽  
And Staphylococcus Aureus

Propolis is a popular subject of research worldwide due to its therapeutic potential. The antimicrobial activity of propolis appears to be promising but depends on many variables related to its origin such as the content of phenolics and flavonoids. To address this issue with Croatian propolis, which has two major origins (Mediterranean and continental), we exposed bacteria (Escherichia coli and Staphylococcus aureus) and yeasts (Candida albicans and Aspergillus niger) to different propolis concentrations (two-fold microdilution method with TCC/formazan endpoint). Total phenolic and flavonoid content and chromatographic profile along with antioxidant activity were assessed. The majority of the 24 propolis samples tested exhibited potent antimicrobial activity against S. aureus bacteria and the yeast C. albicans. Most propolis samples also exhibited robust antioxidative capacity which correlated polyphenol and flavonoid content. To the best of our knowledge this is the first study in which the antimicrobial activity of Croatian propolis is correlated with its constituents.

scholarly journals Assessment of Total Phenolic and Flavonoid Contents and Potential Biological Efficacy of Few Pinus species Growing in Northern Himalayas

2019 ◽  
Author(s):  
LAV SINGH ◽  
Pooja Dixit ◽  
Atul Kumar Upadhyay ◽  
Ravi Prakash Srivastava ◽  
Shiva raman Pandey ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Total Phenolics ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Environmental Interventions ◽  
Disk Diffusion Test ◽  
Pharmacological Significance ◽  
Solvent Systems ◽  
Antioxidant And Antimicrobial Activity ◽  
Phenolic And Flavonoid

Environmental interventions and ecological adaptations harbor millions of valued substances and metabolites in plants which can be employed and commercialized for human benefits. Present study encompasses the untapped potential of pine needles of Indo-Himalayan region for the production of different metabolites and their pharmacological significance in terms of antioxidant and antimicrobial activity. Total phenolic and flavonoid content from the needles of ten pine species was quantified using three different solvent systems. Results revealed that out of 10 different selected Pinus species, Pinus taeda showed highest concentration of total phenolics, soluble-F phenolics and flavonoids content (approx.147.02 mg/g, 141.08 mg/g and 21.91mg/g respectively) as compared to other species. On the other hand P. greggii showed highest Bound-W phenolic content (approx.3.62mg/g). Among all the selected plant species, the needles of P.echinata exhibited the highest and P.thunbergii had the lowest ratio of total flavonoids to total phenolics. Most of these compounds were found to have effective antioxidant activities as well as antimicrobial activity, as estimated by oxygen radical absorbance capacity (ORAC) and disk diffusion test respectively.

scholarly journals Fatty acids, bioactive substances, antioxidant and antimicrobial activity of Ankyropetalum spp., a novel source of nervonic acid

2021 ◽  
Vol 72 (1) ◽  
pp. e399
Author(s):  
N. Comlekcioglu ◽  
M. Kutlu
Keyword(s):  
Antimicrobial Activity ◽  
Candida Parapsilosis ◽  
Total Phenolic ◽  
Nervonic Acid ◽  
Plant Oil ◽  
Bioactive Substances ◽  
Ultrasonic Bath ◽  
Test Organisms ◽  
Antioxidant And Antimicrobial Activity ◽  
Phenolic And Flavonoid

Ankyropetalum extracts were obtained by using two different extractors (Soxhlet and ultrasonic bath). The phenol, flavonoid, DPPH, FRAP, and antimicrobial activity properties of the extracts were investigated. In addition, the fatty acid composition was determined in GC-MS. High values were found in A. reuteri and A. gypsophiloides for total phenolic and flavonoid contents, respectively. DPPH and FRAP values were high in A. arsusianum and A. gypsophiloides, respectively. Better results were obtained by using methanol as the solvent and soxhlet as the extractor. The results showed that the extracts seem to be reasonably effective against test organisms including clinical isolates. The most promising results were obtained with all species USB extracts against Candida parapsilosis. It is notable that the levels of nervonic acid in A. arsusianum and A. reuteri reached 40%. Unlike other sources of nervonic acid in the world, the absence of erucic acid in plant oil increases the value of these plants.

The influence of Gum Arabic on the physicochemical and antimicrobial activity of the microencapsulated Mahkota Dewa (Phaleria macrocarpa) leaves

Food Research ◽  
2021 ◽  
Vol 5 (3) ◽  
pp. 203-213
Author(s):  
Z. Mohd Zin ◽  
N.H. Razman ◽  
Hasmadi M. ◽  
M.N. Abd Manap ◽  
M.K. Zainol
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Gum Arabic ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Spherical Structure ◽  
Ultrasonic Spray ◽  
Phaleria Macrocarpa ◽  
Coating Matrix ◽  
Phenolic And Flavonoid

Mahkota Dewa (Phaleria macrocarpa) also known as God's Crown has been historically used as an indispensable alternative herbal medicine. Microencapsulation is a process whereby tiny particles or droplets are engulfed or enclosed in a coating matrix to produce small capsules. Generally, without microencapsulation, powders are fragile materials that could easily interfere with other components that are difficult to dissolve in water, lose their beneficial properties and decrease shelf life. It is hoped that the microencapsulation would increase the consistency of the powder during storage and maintain its beneficial properties. The goal of this research is to investigate the physicochemical and antimicrobial activity of Mahkota Dewa leaves encapsulated in different concentrations of gum Arabic (GA) and to determine the form of antioxidant and their role and properties. Mahkota Dewa leaves powders were microencapsulated in 0%, 2%, 4%, 6%, 8% and 10% gum Arabic using an ultrasonic spray dryer at 90οC. The microencapsulated Mahkota Dewa leaves (MMDL) samples were subjected to physicochemical and antimicrobial activity. The results showed that the 6% GA MMDL exhibited the highest yield (3.91%) while 0% GA was the lowest yield (1.64%). The highest total phenolic and flavonoid content was exhibited by 2% GA. The highest DPPH inhibition was depicted in 0% GA which indicates the highest antioxidant activity (54.9±0.01%) and is significantly (p<0.05) different from other samples. The highest inhibition was exhibited in 0% GA in the TBA method and FTC analysis. The encapsulated powders were identified to have weak antimicrobial activity against Bacillus cereus, Escherichia coli, Staphylococcus aureus, Salmonella and Listeria monocytogenes. The powders produced have an irregularly spherical structure and smooth surface with some dented spots on the surface. The different concentration of gum Arabic resulted in different antioxidant activity, flavonoid content and antimicrobial activity of MMDL.

scholarly journals A STUDY ON PHYTOCHEMICAL SCREENING, ANTIOXIDANT, ANTIMICROBIAL AND α-ΑMYLASE INHIBITORY ACTIVITIES OF CRUDE EXTRACTS OF THE STEM BARK OF PISONIA GRANDIS, R.BR

2017 ◽  
Vol 10 (11) ◽  
pp. 129
Author(s):  
Vishu Mohan ◽  
Mohan Kumar Ramasamy ◽  
Srikalyani Vemuri ◽  
Ilango Kaliappan
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Stem Bark ◽  
Antidiabetic Activity ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Flavonoid Content ◽  
Phenolic And Flavonoid

  Objective: The aim of the present study is to determine the phytochemical screening, antioxidant activity and α-amylase inhibitory activity of the crude hexane, ethyl acetate and ethanolic stem bark extract of Pisonia grandis.Methods: The evaluation of antioxidant and antimicrobial activity, total phenolic, and flavonoid content were assessed using 2,2-diphenyl-1- picrylhydrazyl, Folin–Ciocalteu’s reagent, and aluminum chloride assay, respectively. The antidiabetic activity was assessed for porcine pancreatic α-amylase for the stem bark of P. grandis. Results: Phytochemical screening confirmed the presence of phenolic, flavonoids, tannins, saponins, terpenoids, and steroids in all the three extracts. The antioxidant activity showed 148.2 μg/ml, total phenolic content (gallic acid equivalent), 0.0665±0.0002 mg/g, flavonoid content (quercetin equivalent), 0.6061±0.1817 mg/g, and inhibitory concentration 50% values were found to be 40.42 μg/ml and showed better in ethyl acetate extract. The antidiabetic activity exhibited mimic action with insulin due to the presence of pinnatol in the stem bark and leaves of P. grandis. Conclusion: P. grandis stem bark crude ethyl acetate extract showed strong antioxidant activity, high phenolic, and flavonoid content. The antimicrobial activity was studied in both Gram-positive and Gram-negative strains against ampicillin and rifampicin as reference drugs. Antidiabetic activity shows effective result by α-amylase inhibitory activity.

The Comparative Study of Equisetum pratense, E. sylvaticum, E. telmateia: Accumulation of Silicon, Antioxidant and Antimicrobial Screening

2019 ◽  
Vol 70 (7) ◽  
pp. 2519-2523
Author(s):  
Denisa Batir Marin ◽  
Oana Cioanca ◽  
Mihai Apostu ◽  
Cristina Gabriela Tuchilus ◽  
Cornelia Mircea ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Methanol Extract ◽  
Previous Treatment ◽  
Total Phenolic ◽  
Quantitative Chemical Analysis ◽  
Qualitative And Quantitative ◽  
Antioxidant And Antimicrobial Activity ◽  
The Comparative Study

The objective of the current study is represented by the determination of silica and a phytochemical screening of phenolic derivates of some Equisetum species. The antioxidant and antimicrobial activity for Equisetum pratense Ehrh.,, Equisetum sylvaticum L. and Equisetum telmateia Ehrh. (sin. Equisetum maximum Lam.) were also investigated. The concentration of silicon (Si) in plants was determined by the spectrophotometric method using previous treatment with NaOH 50% both for the stem and the nodal branches [1]. Results obtained varied from 95.12 to 162.10 SiO2 mg/g dry plant which represents 4.44% to 7.58% Si/100g dry sample. Two types of total extracts were obtained using different solvents and were subjected to qualitative and quantitative chemical analysis considering total phenolic content [2]. The highest concentration of investigated compounds was found in the methanolic extract, E. sylvaticum, 196.5mg/g dry sample. Antioxidant activity was monitored spectrophotometrically and expressed in terms of IC50 (�g/mL) [3]. Values gathered ranged from 261.7 to 429.5 �g/mL. The highest capacity to neutralized DPPH radicals was found in E. sylvaticum. In vitro antimicrobial activity was determined using difusimetric method [4]. Testing was performed on four microorganisms: three strains of bacteria and one species of fungi. Different effects were noticed against the bacteria, furthermore the methanol extract appeared to be most efficient. All extracts showed significand antimicrobial activity against Staphylococcus aureus (ATCC 25923) and Candida albicans (ATCC 90028) and weak to no activity against Pseudomonas aeruginosa (ATCC 27853) and Escherichia coli (ATCC 25922).

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