scholarly journals Ribosome display: a potent display technology used for selecting and evolving specific binders with desired properties

2018 ◽  
Author(s):  
Ruowei Li ◽  
Guangbo Kang ◽  
Min Hu ◽  
He Huang
Keyword(s):  
High Performance ◽  
Life Science ◽  
Yeast Display ◽  
Ribosome Display ◽  
Science Field ◽  
Display Technology ◽  
Selection For ◽  
Display Systems ◽  
Development Prospects

Currently, a variety of display technologies have been developed in the life science field, such as phage display, ribosome display, and yeast display. Many studies have found that display technologies are powerful and universal methods when they are combined with large genetically encoded binder libraries, which results in the generation of high-performance binders against nearly any antigen interested. As a result, display technologies are widely applied to molecular biology, clinic and medicine. Ribosome display based on cell-free display systems has been established as a different type of technology for 23 years until now. Compared to other related methods, ribosome display possesses unique advantages and is successfully exploited to the selection for functional and specific binders in vitro, exhibiting potent development prospects. Here in this paper, we will review the theories and advantages of ribosome display, and will highlight how it is being used now to select and evolve functional proteins as well as applications in diagnostics and therapeutics.

scholarly journals Ribosome display: a potent display technology used for selecting and evolving specific binders with desired properties

2018 ◽  
Author(s):  
Ruowei Li ◽  
Guangbo Kang ◽  
Min Hu ◽  
He Huang
Keyword(s):  
High Performance ◽  
Life Science ◽  
Yeast Display ◽  
Ribosome Display ◽  
Science Field ◽  
Display Technology ◽  
Selection For ◽  
Display Systems ◽  
Development Prospects

Currently, a variety of display technologies have been developed in the life science field, such as phage display, ribosome display, and yeast display. Many studies have found that display technologies are powerful and universal methods when they are combined with large genetically encoded binder libraries, which results in the generation of high-performance binders against nearly any antigen interested. As a result, display technologies are widely applied to molecular biology, clinic and medicine. Ribosome display based on cell-free display systems has been established as a different type of technology for 23 years until now. Compared to other related methods, ribosome display possesses unique advantages and is successfully exploited to the selection for functional and specific binders in vitro, exhibiting potent development prospects. Here in this paper, we will review the theories and advantages of ribosome display, and will highlight how it is being used now to select and evolve functional proteins as well as applications in diagnostics and therapeutics.

scholarly journals Ribosome Display Technology: Applications in Disease Diagnosis and Control

Antibodies ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 28
Author(s):  
Adinarayana Kunamneni ◽  
Christian Ogaugwu ◽  
Steven Bradfute ◽  
Ravi Durvasula
Keyword(s):  
In Vitro Selection ◽  
Low Cost ◽  
Disease Diagnosis ◽  
Single Chain ◽  
Single Chain Antibody ◽  
Ribosome Display ◽  
Technology Applications ◽  
Display Technology ◽  
And Control

Antibody ribosome display remains one of the most successful in vitro selection technologies for antibodies fifteen years after it was developed. The unique possibility of direct generation of whole proteins, particularly single-chain antibody fragments (scFvs), has facilitated the establishment of this technology as one of the foremost antibody production methods. Ribosome display has become a vital tool for efficient and low-cost production of antibodies for diagnostics due to its advantageous ability to screen large libraries and generate binders of high affinity. The remarkable flexibility of this method enables its applicability to various platforms. This review focuses on the applications of ribosome display technology in biomedical and agricultural fields in the generation of recombinant scFvs for disease diagnostics and control.

In Vitro Selection for Catalytic Activity with Ribosome Display

2002 ◽  
Vol 124 (32) ◽  
pp. 9396-9403 ◽  
Author(s):  
Patrick Amstutz ◽  
Joelle N. Pelletier ◽  
Armin Guggisberg ◽  
Lutz Jermutus ◽  
Sandro Cesaro-Tadic ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
In Vitro Selection ◽  
Ribosome Display ◽  
Selection For

scholarly journals A Survey of Methylobacterium Species and Strains Reveals Widespread Production and Varying Profiles of Cytokinin Phytohormones

2021 ◽  
Author(s):  
Daniel Palberg ◽  
Anna Kisiała ◽  
Gabriel Lemes Jorge ◽  
R. J. Neil Emery
Keyword(s):  
High Performance ◽  
Active Form ◽  
Plant Growth Promoting Bacteria ◽  
Liquid Chromatography Tandem Mass ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Selection For ◽  
Indole 3 Acetic Acid

Abstract BackgroundSymbiotic Methylobacterium strains comprise a significant part of plant microbiomes. Their presence enhances plant productivity and stress resistance, prompting classification of these strains as plant growth-promoting bacteria (PGPB). Methylobacteria can synthesize unusually high levels of plant hormones, called cytokinins (CKs), including the most active form, trans-Zeatin (tZ). ResultsThis study provides a comprehensive inventory of 46 representatives of Methylobacterium genus with respect to phytohormone production in vitro, including 16 CK forms, abscisic acid (ABA) and indole-3-acetic acid (IAA). High performance-liquid chromatography - tandem mass spectrometry (HPLC-MS/MS) analyses revealed varying abilities of Methylobacterium strains to secrete phytohormones that ranged from 5.09 to 191.47 pmol mL-1 for total CKs, and 0.46 to 82.16 pmol mL-1 for tZ. Results indicate that reduced methanol availability, the sole carbon source for bacteria in the medium, stimulates CK secretion by Methylobacterium. Additionally, select strains were able to transform L-tryptophan into IAA while no ABA production was detected.ConclusionsTo better understand features of CKs in plants, this study uncovers CK profiles of Methylobacterium that are instrumental in microbe selection for effective biofertilizer formulations.

scholarly journals Introduction pages

2021 ◽  
Vol 13 (1) ◽  
pp. 10926
Author(s):  
Radu E. SESTRAS
Keyword(s):  
Life Science ◽  
Albino Rats ◽  
Efficient Production ◽  
Pharmacological Activities ◽  
Methanol Fraction ◽  
Vegetative Organs ◽  
Pachyrhizus Erosus ◽  
Natural Pesticides ◽  
Selection For

Notulae Scientia Biologicae (http://www.notulaebiologicae.ro), Issue 1, Volume 13, 2021: The papers published in this issue represent interesting novelties in different topics of life science. Among the exciting researches or reviews, we invite readers to find news about: Phytochemistry and pharmacological activities of Saponaria officinalis L.; The Spanish chamomile (Anacyclus pyrethrum) and pyrethrum (Tanacetum cineraiifolium): organic and natural pesticides and treasure of medicinal herbs; Prophylactic effect of the aqueous extract of Pimpinella anisum on the behavior of Wistar rats exposed to mercury; Anti-ulcer property of methanol fraction of Callichilia subsessilis leaf extract in albino rats; Nesting tree selection by scattered heronry birds of drought prone Northern Bankura, West Bengal, India: preference of Phoenix sylvestris near wetland-associated habitats; Explants selection for in vitro propagation of Pachyrhizus erosus L.; Silene thymifolia Sibth. et Sm. (Caryophyllaceae) – A vulnerable species in Romania: Anatomical aspects of vegetative organs.   Announcement: From Volume 13, Issue 1, 2021, Notulae Scientia Biologicae journal will use article numbers in place of the traditional method of continuous pagination through the volume. This step helps us to maintain a rapid, efficient production process by being able to define pagination as soon as a paper is accepted. For papers that use article numbers, the page number of full-text articles will start from 1 to the last page and the citation needs only to list the article number, consequently, instead of the classic style of the journal citation.

Development of high-performance objective lens polepiece for ultrahigh resolution Analytical Electron Microscope

1993 ◽  
Vol 51 ◽  
pp. 254-255
Author(s):  
K. Fukushima ◽  
T. Kaneyama ◽  
F. Hosokawa ◽  
H. Tsuno ◽  
T. Honda ◽  
...  
Keyword(s):  
Electron Microscope ◽  
High Performance ◽  
Open Space ◽  
Materials Science ◽  
Objective Lens ◽  
Ultrahigh Resolution ◽  
Science Field ◽  
Specimen Holder ◽  
Analytical Electron ◽  
Analytical Electron Microscope

Recently, in the materials science field, the ultrahigh resolution analytical electron microscope (UHRAEM) has become a very important instrument to study extremely fine areas of the specimen. The requirements related to the performance of the UHRAEM are becoming gradually severer. Some basic characteristic features required of an objective lens are as follows, and the practical performance of the UHRAEM should be judged by totally evaluating them.1) Ultrahigh resolution to resolve ultrafine structure by atomic-level observation.2) Nanometer probe analysis to analyse the constituent elements in nm-areas of the specimen.3) Better performance of x-ray detection for EDS analysis, that is, higher take-off angle and larger detection solid angle.4) Higher specimen tilting angle to adjust the specimen orientation.To attain these requirements simultaneously, the objective lens polepiece must have smaller spherical and chromatic aberration coefficients and must keep enough open space around the specimen holder in it.

Co-purification of bone resorbing activity and adenylate cyclase stimulating activity from human tumours associated with the humoral hypercalcaemia of malignancy

1987 ◽  
Vol 114 (1) ◽  
pp. 18-26 ◽  
Author(s):  
Chohei Shigeno ◽  
Itsuo Yamamoto ◽  
Shegiharu Dokoh ◽  
Megumu Hino ◽  
Jun Aoki ◽  
...  
Keyword(s):  
Bone Resorption ◽  
High Performance ◽  
Basic Protein ◽  
Gel Filtration ◽  
Exchange Chromatography ◽  
Protein Factor ◽  
Human Tumours ◽  
Acid Stable ◽  
Bone Resorbing Activity

Abstract. We have partially purified a tumour factor capable of stimulating both bone resorption in vitro and cAMP accumulation in osteoblastic ROS 17/2 cells from three human tumours associated with humoral hypercalcaemia of malignancy. Purification of tumour factor by sequential acid urea extraction, gel filtration and cation-exchange chromatography, reverse-phase high performance liquid chromatography followed by analytical isoelectric focussing provided a basic protein (pI > 9.3) with a molecular weight of approximately 13 000 as a major component of the final preparation which retained both the two bioactivities. Bone resorbing activity and cAMP-increasing activity in purified factor correlated with each other. cAMP-increasing activity of the factor was heat- and acid-stable, but sensitive to alkaline ambient pH. Treatment with trypsin destroyed cAMP-increasing activity of the factor. Synthetic parathyroid hormone (PTH) antagonist, human PTH-(3– 34) completely inhibited the cAMP-increasing activity of the factor. The results suggest that this protein factor, having its effects on both osteoclastic and osteoblastic functions, may be involved in development of enhanced bone resorption in some patients with humoral hypercalcaemia of malignancy.

Improvement of effectiveness in maize breeding

2006 ◽  
Vol 54 (3) ◽  
pp. 351-358 ◽  
Author(s):  
P. Pepó
Keyword(s):  
Recurrent Selection ◽  
Genetic Manipulation ◽  
Field Testing ◽  
Tissue Cultures ◽  
Maize Breeding ◽  
Breeding Programmes ◽  
Speed Up ◽  
Selection For

Plant regeneration via tissue culture is becoming increasingly more common in monocots such as maize (Zea mays L.). Pollen (gametophytic) selection for resistance to aflatoxin in maize can greatly facilitate recurrent selection and the screening of germplasm for resistance at much less cost and in a shorter time than field testing. In vivo and in vitro techniques have been integrated in maize breeding programmes to obtain desirable agronomic attributes, enhance the genes responsible for them and speed up the breeding process. The efficiency of anther and tissue cultures in maize and wheat has reached the stage where they can be used in breeding programmes to some extent and many new cultivars produced by genetic manipulation have now reached the market.

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