scholarly journals Spontaneous evolution of human skin fibroblasts into wound-healing keratinocyte-like cells

Theranostics ◽  
2019 ◽  
Vol 9 (18) ◽  
pp. 5200-5213 ◽  
Author(s):  
Fang Zhang ◽  
Dandan Zhang ◽  
Kai Cheng ◽  
Zaixin Zhou ◽  
Shupeng Liu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Human Skin ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Spontaneous Evolution

scholarly journals Root extractive from Daphne genkwa benefits in wound healing of anal fistula through up-regulation of collagen genes in human skin fibroblasts

2017 ◽  
Vol 37 (2) ◽  
Author(s):  
Dong Yang ◽  
Jun-hua Xu ◽  
Ren-jie Shi
Keyword(s):  
Wound Healing ◽  
Human Skin ◽  
Anal Fistula ◽  
Healing Time ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Protein Levels ◽  
Daphne Genkwa

Wound healing is the main problem in the therapy of anal fistula (AF). Daphne genkwa root has been traditionally used as an agent to soak sutures in operation of AF patients, but its function in wound healing remains largely unclear. The aim of the present study was to illuminate mechanisms of D. genkwa root treatment on AF. In the present study, 60 AF patients after surgery were randomly divided into two groups, external applied with or without the D. genkwa extractive. Wound healing times were compared and granulation tissues were collected. In vitro, we constructed damaged human skin fibroblasts (HSFs) with the treatment of TNF-α (10 μg/ml). Cell Count Kit-8 (CCK-8) and flow cytometry analysis were used to determine the effects of D. genkwa root extractive on cell viability, cell cycle and apoptosis of damaged HSFs. Furthermore, protein levels of TGF-β, COL1A1, COL3A1, Timp-1, matrix metalloproteinase (MMP)-3 (MMP-3) and MEK/ERK signalling pathways were investigated both in vivo and in vitro. Results showed that D. genkwa root extractive greatly shortens the wound healing time in AF patients. In granulation tissues and HSFs, treatment with the extractive significantly elevated the expressions of COL1A1, COL3A1, Timp-1, c-fos and Cyclin D1, while reduced the expression of MMP-3. Further detection presented that MEK/ERK signalling was activated after the stimulation of extractive in HSFs. Our study demonstrated that extractive from D. genkwa root could effectively improve wound healing in patients with AF via the up-regulation of fibroblast proliferation and expressions of COL1A1 and COL3A1.

scholarly journals WOUND HEALING PROPERTIES OF SWIETENIA MAHAGONI SEED EXTRACTED USING SCCO2: AN IN VITRO STUDY

2018 ◽  
Vol 81 (1) ◽  
Author(s):  
Hartati Hartati ◽  
Liza Md Salleh ◽  
Irma Suryani Idris ◽  
Andi Asmawati Azis
Keyword(s):  
Wound Healing ◽  
Human Skin ◽  
In Vitro Study ◽  
Seed Extract ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Scratch Assay ◽  
Healing Agent ◽  
Swietenia Mahagoni

Swietenia mahagoni seed has been traditionally used as a wound healing agent. The aim of this study was to investigate the in vitro healing properties of Swietenia mahagoni seed extract. A supercritical carbon dioxide (SCCO2) extract of Swietenia mahagoni seeds was tested regarding its ability to stimulate the growth of human skin fibroblasts (HSF 1184) in vitro using the colorimetric methylthiazol tetrazolium (MTT) assay and scratch assay in a selected range of extract concentrations. The results show that the Swietenia mahagoni seed extract at a concentration of 0.01 mg/mL was able to stimulate the growth of human skin fibroblasts. The scratch assay showed that all of the extracts led to a significantly higher percentage of wound closure compared to the control. The wound healing capabilities of Swietenia mahagoni seed extract may be due to its fibroblast stimulation activity, which may promote repair of the wounded dermis. Thus, it may serve as a good herbal component to incorporate into products designed to promote wound healing.

Sign in / Sign up

Export Citation Format

Share Document