scholarly journals Response of Human Skin Fibroblasts to Mechanical Stretch in Wound Healing Process Analyzed Using a Three-Dimensional Culture Model

2015 ◽  
Vol 4 (0) ◽  
pp. 170-178 ◽  
Author(s):  
Kaori Shikano ◽  
Keisuke Chiba ◽  
Shogo Miyata
Keyword(s):  
Wound Healing ◽  
Human Skin ◽  
Healing Process ◽  
Three Dimensional ◽  
Mechanical Stretch ◽  
Skin Fibroblasts ◽  
Wound Healing Process ◽  
Human Skin Fibroblasts ◽  
Culture Model ◽  
Three Dimensional Culture

scholarly journals Root extractive from Daphne genkwa benefits in wound healing of anal fistula through up-regulation of collagen genes in human skin fibroblasts

2017 ◽  
Vol 37 (2) ◽  
Author(s):  
Dong Yang ◽  
Jun-hua Xu ◽  
Ren-jie Shi
Keyword(s):  
Wound Healing ◽  
Human Skin ◽  
Anal Fistula ◽  
Healing Time ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Protein Levels ◽  
Daphne Genkwa

Wound healing is the main problem in the therapy of anal fistula (AF). Daphne genkwa root has been traditionally used as an agent to soak sutures in operation of AF patients, but its function in wound healing remains largely unclear. The aim of the present study was to illuminate mechanisms of D. genkwa root treatment on AF. In the present study, 60 AF patients after surgery were randomly divided into two groups, external applied with or without the D. genkwa extractive. Wound healing times were compared and granulation tissues were collected. In vitro, we constructed damaged human skin fibroblasts (HSFs) with the treatment of TNF-α (10 μg/ml). Cell Count Kit-8 (CCK-8) and flow cytometry analysis were used to determine the effects of D. genkwa root extractive on cell viability, cell cycle and apoptosis of damaged HSFs. Furthermore, protein levels of TGF-β, COL1A1, COL3A1, Timp-1, matrix metalloproteinase (MMP)-3 (MMP-3) and MEK/ERK signalling pathways were investigated both in vivo and in vitro. Results showed that D. genkwa root extractive greatly shortens the wound healing time in AF patients. In granulation tissues and HSFs, treatment with the extractive significantly elevated the expressions of COL1A1, COL3A1, Timp-1, c-fos and Cyclin D1, while reduced the expression of MMP-3. Further detection presented that MEK/ERK signalling was activated after the stimulation of extractive in HSFs. Our study demonstrated that extractive from D. genkwa root could effectively improve wound healing in patients with AF via the up-regulation of fibroblast proliferation and expressions of COL1A1 and COL3A1.

scholarly journals Hydrogels for management of chronic wound healing

2021 ◽  
Vol 5 (3) ◽  
pp. 095-104
Author(s):  
IM Cardoso-Daodu ◽  
CP Azubuike ◽  
MO Ilomuanya
Keyword(s):  
Wound Healing ◽  
Chronic Wounds ◽  
Healing Process ◽  
Three Dimensional ◽  
Scar Tissue ◽  
The Body ◽  
Polymer Chains ◽  
Wound Healing Process ◽  
Dimensional Network ◽  
Healing Wound

Chronic wounds occur when one wound healing process or a sequence of wound healing events are affected resulting in slow healing of the wound thereby placing the patient in deep pain. Various diseases and conditions can delay the process of wound healing. Wound healing can be classified into four main stages: hemostasis, inflammation, remodeling, and scar tissue formation with each phase overlapping one another. The skin is the largest organ in the body. It protects the entire external surface of the human body and is the primary site of interaction with the outside environment. There is therefore a need to fabricate an ideal dressing through scientific research and investigations. Hydrogels are a three-dimensional network of hydrophilic polymers that can swell in water and absorb copious amounts of water while maintaining their structure because of their chemical or physical crosslinking of individual polymer chains. A hydrogel must be composed of at least 10% water. Hydrogels possess the flexibility and water percentage which is remarkably like tissues. They are biocompatible and biodegradable which makes them ideal for dermal wound healing.

scholarly journals Spontaneous evolution of human skin fibroblasts into wound-healing keratinocyte-like cells

Theranostics ◽  
2019 ◽  
Vol 9 (18) ◽  
pp. 5200-5213 ◽  
Author(s):  
Fang Zhang ◽  
Dandan Zhang ◽  
Kai Cheng ◽  
Zaixin Zhou ◽  
Shupeng Liu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Human Skin ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Spontaneous Evolution

scholarly journals Collagenase-3 (MMP-13) Enhances Remodeling of Three-Dimensional Collagen and Promotes Survival of Human Skin Fibroblasts

2007 ◽  
Vol 127 (1) ◽  
pp. 49-59 ◽  
Author(s):  
Mervi J. Toriseva ◽  
Risto Ala-aho ◽  
Jarkko Karvinen ◽  
Andrew H. Baker ◽  
Varpu S. Marjomäki ◽  
...  
Keyword(s):  
Human Skin ◽  
Three Dimensional ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts

scholarly journals Monitoring of wound healing process of human skin after fractional laser treatments with optical coherence tomography

2013 ◽  
Vol 4 (11) ◽  
pp. 2362 ◽  
Author(s):  
Meng-Tsan Tsai ◽  
Chih-Hsun Yang ◽  
Su-Chin Shen ◽  
Ya-Ju Lee ◽  
Feng-Yu Chang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Optical Coherence Tomography ◽  
Human Skin ◽  
Healing Process ◽  
Wound Healing Process ◽  
Optical Coherence ◽  
Fractional Laser ◽  
Laser Treatments
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