Effects of WISP-1 on Matrix Metalloproteinase Expression and Wound Healing in Human Skin Fibroblasts via Down-Regulation of miR-519d

2015 ◽  
Vol 7 (4) ◽  
pp. 202-209
Author(s):  
Kuan-Yun Chen
Keyword(s):  
Wound Healing ◽  
Matrix Metalloproteinase ◽  
Human Skin ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Down Regulation

4-Methylumbelliferone induces the expression of membrane type 1-matrix metalloproteinase in cultured human skin fibroblasts

2002 ◽  
Vol 298 (5) ◽  
pp. 646-650 ◽  
Author(s):  
Toshiya Nakamura ◽  
Takashi Ishikawa ◽  
Naoki Nanashima ◽  
Tomisato Miura ◽  
Hiroyuki Nozaka ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Human Skin ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Membrane Type

scholarly journals Root extractive from Daphne genkwa benefits in wound healing of anal fistula through up-regulation of collagen genes in human skin fibroblasts

2017 ◽  
Vol 37 (2) ◽  
Author(s):  
Dong Yang ◽  
Jun-hua Xu ◽  
Ren-jie Shi
Keyword(s):  
Wound Healing ◽  
Human Skin ◽  
Anal Fistula ◽  
Healing Time ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Protein Levels ◽  
Daphne Genkwa

Wound healing is the main problem in the therapy of anal fistula (AF). Daphne genkwa root has been traditionally used as an agent to soak sutures in operation of AF patients, but its function in wound healing remains largely unclear. The aim of the present study was to illuminate mechanisms of D. genkwa root treatment on AF. In the present study, 60 AF patients after surgery were randomly divided into two groups, external applied with or without the D. genkwa extractive. Wound healing times were compared and granulation tissues were collected. In vitro, we constructed damaged human skin fibroblasts (HSFs) with the treatment of TNF-α (10 μg/ml). Cell Count Kit-8 (CCK-8) and flow cytometry analysis were used to determine the effects of D. genkwa root extractive on cell viability, cell cycle and apoptosis of damaged HSFs. Furthermore, protein levels of TGF-β, COL1A1, COL3A1, Timp-1, matrix metalloproteinase (MMP)-3 (MMP-3) and MEK/ERK signalling pathways were investigated both in vivo and in vitro. Results showed that D. genkwa root extractive greatly shortens the wound healing time in AF patients. In granulation tissues and HSFs, treatment with the extractive significantly elevated the expressions of COL1A1, COL3A1, Timp-1, c-fos and Cyclin D1, while reduced the expression of MMP-3. Further detection presented that MEK/ERK signalling was activated after the stimulation of extractive in HSFs. Our study demonstrated that extractive from D. genkwa root could effectively improve wound healing in patients with AF via the up-regulation of fibroblast proliferation and expressions of COL1A1 and COL3A1.

Differential down-regulation of COX-2 and MMP-13 in human skin fibroblasts by glucosamine-hydrochloride

2009 ◽  
Vol 56 (1) ◽  
pp. 43-50 ◽  
Author(s):  
Hua Hong ◽  
Yu-Kyoung Park ◽  
Mi-Sun Choi ◽  
Nam-Hee Ryu ◽  
Dae-Kyu Song ◽  
...  
Keyword(s):  
Human Skin ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Down Regulation ◽  
Glucosamine Hydrochloride ◽  
Cox 2

Biflavonoids isolated from Selaginella tamariscina regulate the expression of matrix metalloproteinase in human skin fibroblasts

2008 ◽  
Vol 16 (2) ◽  
pp. 732-738 ◽  
Author(s):  
Chan-Woo Lee ◽  
Hyun-Jung Choi ◽  
Han-Sung Kim ◽  
Duck-Hee Kim ◽  
Ih-Seop Chang ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Human Skin ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Selaginella Tamariscina
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