scholarly journals A Novel Protein Corona Characterization based on the Reflectometric Interference Spectroscopy with Silica Colloidal Crystal Films

2021 ◽  
Vol 79 (3) ◽  
pp. 338
Author(s):  
Feng Wu ◽  
Qianqian Su ◽  
Lele Zhou ◽  
Pengfei Xu ◽  
Ao Dong ◽  
...  
2019 ◽  
Vol 91 (9) ◽  
pp. 6080-6087 ◽  
Author(s):  
Qianqian Su ◽  
Feng Wu ◽  
Pengfei Xu ◽  
Ao Dong ◽  
Chang Liu ◽  
...  

2017 ◽  
Vol 9 (48) ◽  
pp. 42075-42083
Author(s):  
Jennifer L. Russell ◽  
Thomas E. Mallouk

Langmuir ◽  
2008 ◽  
Vol 24 (18) ◽  
pp. 10532-10536 ◽  
Author(s):  
Jeffrey L. Moran ◽  
Philip M. Wheat ◽  
Jonathan D. Posner

NANO ◽  
2021 ◽  
pp. 2150052
Author(s):  
Feng Wu ◽  
Qianqian Su ◽  
Lele Zhou ◽  
Pengfei Xu ◽  
Ao Dong ◽  
...  

When a drug enters an organism, interactions between the drug and proteins in the organism play a vital role in the storage, transport and metabolism of the drug and also affect its nonspecific toxicity, targeting and pharmacodynamic activity. However, monitoring the interaction process is a great challenge in the research of the absorption, transport and metabolic processes of drugs. In this study, we used reflectometric interference spectroscopy (RIfS) and silica colloidal crystal (SCC) film as a sensing platform to detect the binding affinity between human serum albumin (HSA) and indomethacin. SCC films composed of three silica nanospheres with different diameters were fabricated using the vertical evaporation method. HSA was immobilized covalently on SCC film using a very simple approach, and optical thickness was used as a parameter to evaluate the process of drug absorption and desorption. Finally, the optimal SCC film was selected, and three drugs other than indomethacin (i.e., warfarin, salicylic acid and quinine) were used for the validation of this sensing platform. The results verified that SCC film using RIfS is a simple and real-time sensing platform for detecting the affinity between HSA and drugs, which may be widely used in drug development and clinical testing in the future.


2021 ◽  
Vol 21 (10) ◽  
pp. 5215-5221
Author(s):  
Ao Dong ◽  
Qianqian Su ◽  
Ning Ma ◽  
Pengfei Xu ◽  
Lele Zhou ◽  
...  

In order for the colloidal crystal films to be better applied, the influence of relative humidity on the preparation of silica colloidal crystal (SCC) films was systematically studied to solve the problem of different thicknesses of SCC films prepared by different batches under the conditions with the same temperature, concentration of suspension and diameter of the particles. SCC films with 190 nm particles were prepared by static vertical deposition method under different humidity regulated by saturated salt solutions, and the thickness of the films was obtained by an interferometric method. The results showed that the increase in humidity would reduce the thickness of the prepared films, which was believed to be caused by the decrease in evaporation rate after the wetting film absorbs water vapor. A new formula for calculating film thickness was proposed and verified from a series of experiments. With the control of humidity, high-quality SCC films with controlled thickness can be repeatedly prepared.


2018 ◽  
Vol 160 ◽  
pp. 926-935 ◽  
Author(s):  
Steffen Vowinkel ◽  
Anna Boehm ◽  
Timmy Schäfer ◽  
Torsten Gutmann ◽  
Emanuel Ionescu ◽  
...  

2020 ◽  
Vol 5 (2) ◽  
pp. 1901056 ◽  
Author(s):  
Hua Xu ◽  
Ming Kun Zhang ◽  
Yi Fei Lu ◽  
Jia Jia Li ◽  
Si Jia Ge ◽  
...  

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