scholarly journals Characterization of actinobacteria from the semiarid region, and their antagonistic effect on strains of rhizobia

2017 ◽  
Vol 16 (11) ◽  
pp. 499-507 ◽  
Author(s):  
Vinicius Leite Lima Jose ◽  
Claudia Silveira Martins Suzana ◽  
Aparecida de Siqueira Katia ◽  
Antonio Soares Marcos ◽  
Miranda Martins Claudia
Keyword(s):  
Antagonistic Effect ◽  
Semiarid Region

scholarly journals Characterization of Direct Perturbations on Voltage-Gated Sodium Current by Esaxerenone, a Nonsteroidal Mineralocorticoid Receptor Blocker

Biomedicines ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 549
Author(s):  
Wei-Ting Chang ◽  
Sheng-Nan Wu
Keyword(s):  
Mineralocorticoid Receptor ◽  
Sodium Current ◽  
Ionic Currents ◽  
Antagonistic Effect ◽  
Gh3 Cells ◽  
Excitable Cells ◽  
Voltage Gated ◽  
Ic50 Value ◽  
Low Threshold

Esaxerenone (ESAX; CS-3150, Minnebro®) is known to be a newly non-steroidal mineralocorticoid receptor (MR) antagonist. However, its modulatory actions on different types of ionic currents in electrically excitable cells remain largely unanswered. The present investigations were undertaken to explore the possible perturbations of ESAX on the transient, late and persistent components of voltage-gated Na+ current (INa) identified from pituitary GH3 or MMQ cells. GH3-cell exposure to ESAX depressed the transient and late components of INa with varying potencies. The IC50 value of ESAX required for its differential reduction in peak or late INa in GH3 cells was estimated to be 13.2 or 3.2 μM, respectively. The steady-state activation curve of peak INa remained unchanged during exposure to ESAX; however, recovery of peak INa block was prolonged in the presence 3 μM ESAX. In continued presence of aldosterone (10 μM), further addition of 3 μM ESAX remained effective at inhibiting INa. ESAX (3 μM) potently reversed Tef-induced augmentation of INa. By using isosceles-triangular ramp pulse with varying durations, the amplitude of persistent INa measured at high or low threshold was enhanced by the presence of tefluthrin (Tef), in combination with the appearance of the figure-of-eight hysteretic loop; moreover, hysteretic strength of the current was attenuated by subsequent addition of ESAX. Likewise, in MMQ lactotrophs, the addition of ESAX also effectively decreased the peak amplitude of INa along with the increased current inactivation rate. Taken together, the present results provide a noticeable yet unidentified finding disclosing that, apart from its antagonistic effect on MR receptor, ESAX may directly and concertedly modify the amplitude, gating properties and hysteresis of INa in electrically excitable cells.

Characterization of reactive blue 2 antagonistic effect on vascular ATP receptors

1992 ◽  
Vol 25 ◽  
pp. 164-165
Author(s):  
E. Ragazzi ◽  
A. Chinellato ◽  
L. Pandolfo ◽  
G. Froldi ◽  
L. Caparrotta ◽  
...  
Keyword(s):  
Antagonistic Effect ◽  
Reactive Blue 2 ◽  
Atp Receptors

scholarly journals Characterization of carcasses of Zebu cattle raised in a semiarid region according to sex

2020 ◽  
Vol 49 ◽  
Author(s):  
Filipe Martins Ferraz ◽  
Fredson Vieira e Silva ◽  
Laura Lúcia dos Santos Oliveira ◽  
Iran Borges ◽  
Dorismar David Alves ◽  
...  
Keyword(s):  
Semiarid Region ◽  
Zebu Cattle

scholarly journals Characterization of the insulin-antagonistic effect of growth hormone in man

Diabetologia ◽  
1991 ◽  
Vol 34 (7) ◽  
pp. 500-506 ◽  
Author(s):  
J. Fowelin ◽  
S. Attvall ◽  
H. von Schenck ◽  
U. Smith ◽  
I. Lager
Keyword(s):  
Growth Hormone ◽  
Antagonistic Effect

scholarly journals Characterization of strawberry associated fungi and in vitro antagonistic effect of Trichoderma harzianum

2020 ◽  
Vol 38 (3) ◽  
Author(s):  
Luis Angel Morales-Mora ◽  
Petra Andrade-Hoyos ◽  
M. Angeles Valencia-de Ita ◽  
Omar Romero-Arenas ◽  
Hilda Victoria Silva-Rojas ◽  
...  
Keyword(s):  
Trichoderma Harzianum ◽  
Antagonistic Effect

México es el tercer productor de fresa a nivel mundial, donde la producción de este cultivo es de importancia económica y generación de divisas en el pais. El objetivo de la presente investigación fue identificar y caracterizar morfológicamente los hongos asociados a enfermedades en un cultivo de la fresa, así como determinar la capacidad antagónica <em>in vitro</em> de la cepa T-H4 de <em>Trichoderma harzianum</em> con los hongos identificados. Se colectaron muestras de plantas del cultivo de fresa con síntomas de enfermedades fúngicas, se sembraron en medio PDA y se generaron cultivos monospóricos para su caracterización morfológica. Los hongos identificados y la cepa T-H4 se confrontaron mediante cultivos duales. Se identificaron tres hongos asociados al fruto (<em>A. niger</em>, <em>Colletotrichum</em> sp. y <em>R. stolonifer</em>), tres en hojas y tallo (<em>Pestalotiopsis</em> sp., <em>Curvularia</em> sp. y <em>Alternaria</em> sp.) y dos hongos asociados a la raíz (<em>Rhizoctonia</em> sp. y <em>Fusarium</em> sp.). La cepa T-H4 presentó un nivel antagónico adecuado para <em>Colletotrichum</em> sp., <em>Pestalotiopsis</em> sp., <em>Alternaria</em> sp., <em>Rhizoctonia</em> sp. y <em>Curvularia</em> sp.,<em> in vitro.</em> Se sugiere realizar evaluaciones de control biológico con estos aislamientos en invernadero y a campo abierto, así como determinar su patogenicidad.

scholarly journals MORPHOPHYSIOLOGY OF RHIZOBIA ISOLATED FROM NATIVE FORAGE OF FABACEAE OF THE CAATINGA

2019 ◽  
Vol 32 (4) ◽  
pp. 904-914
Author(s):  
SUELANE DE MELO DIAS ◽  
ALBERÍCIO PEREIRA DE ANDRADE ◽  
ADAILSON PEREIRA DE SOUZA ◽  
ANDRÉ LUIZ RODRIGUES MAGALHÃES ◽  
RISELANE DE LUCENA ALCÂNTARA BRUNO ◽  
...  
Keyword(s):  
High Temperatures ◽  
Native Species ◽  
Growth Period ◽  
Semiarid Region ◽  
Ph Tolerance ◽  
Resistance To Stress ◽  
Macroptilium Atropurpureum ◽  
Physiological Tests ◽  
Exopolysaccharides Production

ABSTRACT The rhizobia found in Fabaceae plants of the Caatinga may have potential as fodder in the semiarid region, thus increasing the availability of feed for animal production in this region. This study aimed to characterize the morphophysiology of rhizobia strains of the forage species Mimosa tenuiflora (Jurema preta), Macroptilium atropurpureum (Siratro), and Desmanthus pernambucanus (Jureminha). The soil used as the substrate was collected at specific locations in the municipalities of Sertânia, Arcoverde, and São Bento do Una, state of Pernambuco. The characterization of rhizobial colonies included growth period, diameter, elevation, transparency, exopolysaccharides production, and color. In addition, salinity, temperature, and pH tolerance tests of the native rhizobia were conducted. Evaluation of cultures showed that the isolates formed colonies with flat elevation, moderate production of exopolysaccharides, coloration varying from cream to white, and a smooth surface. Physiological tests of abiotic stress resistance showed that the native species isolated from M. tenuiflora were more resistant when grown in soils from the municipality of Sertânia and isolates from M. atropurpureum and D. pernambucanus had high resistance to high temperatures, regardless of place of farming. It can be concluded that native rhizobia from the Semiarid region of Pernambuco show resistance to stress caused by high temperatures, salinity variation, and pH. These characteristics are influenced by soil and the botanical species in symbiosis with the rhizobia.

scholarly journals Polyphasic characterization of forage legumes root nodule bacteria isolated from semiarid region in Brazil

2018 ◽  
Vol 41 (3) ◽  
pp. 612-624 ◽  
Author(s):  
Gérsika Fakirra de Oliveira Nunes ◽  
Kelly Alexsandra Souza Menezes ◽  
Aline Araújo Sampaio ◽  
Jakson Leite ◽  
Paulo Ivan Fernandes-Júnior ◽  
...  
Keyword(s):  
Root Nodule ◽  
Semiarid Region ◽  
Nodule Bacteria ◽  
Forage Legumes ◽  
Root Nodule Bacteria ◽  
Polyphasic Characterization

scholarly journals AGRONOMIC AND MORPHOLOGICAL CHARACTERIZATION OF LIPPIA (VERBENACEAE) SPECIES NATIVE FROM BRAZILIAN SEMIARID REGION

2017 ◽  
Vol 92 (2) ◽  
pp. 147
Author(s):  
Ariana Reis Messias Fernandes de Oliveira ◽  
Lenaldo Muniz Oliveira ◽  
José Floriano Barea Pastore ◽  
Tânia Regina dos Santos Silva ◽  
Larissa Correa do Bonfim Costa
Keyword(s):  
Essential Oil ◽  
Agronomic Traits ◽  
Morphological Characterization ◽  
Semiarid Region ◽  
Brazilian Semiarid ◽  
Aromatic Species ◽  
Morphological Differences ◽  
Stem Leaf ◽  
Essential Oil Yield

The genus Lippia L. is represented by several aromatic species, producing essential oils with biological activity, especially antimicrobial.  The semiarid region of Bahia contains several Lippia species that could be economically exploited. This study aimed to characterize morphological and agronomic traits of four species of Lippia native of the semiarid region. The species were collected in a number of cities of the state of Bahia. The experimental design was performed in randomized blocks with four repetitions and four plants per repetition for each species. We conducted quantitative and qualitative morphological characterizations, and evaluated the characters of stem, leaf and inflorescence; in addition to the agronomic characterization, with the fresh and dry matter of inflorescences, we evaluated leaves and stem and essential oil content and yield. The results showed morphological differences between the species for all traits evaluated, except for the number of flowers per inflorescence. The agronomical characterization showed differences for all measured characters, and the species Lippia lasiocalycina was distinguished by the essential oil yield.

Preparation and characterization of natural quercetin-based Mongolia Medicine SenDeng-4 nanoemulsion (N-QUE-NE) and its antibacterial activity..

2020 ◽  
Vol 18 ◽  
Author(s):  
Yanfang Zhang ◽  
Rina Du ◽  
Pengwei Zhao ◽  
Sha Lu ◽  
Rina Wu ◽  
...  
Keyword(s):  
Particle Size ◽  
Antibacterial Activity ◽  
Zeta Potential ◽  
High Speed ◽  
Antibacterial Effect ◽  
Average Particle Size ◽  
Antagonistic Effect ◽  
Dilution Method ◽  
Average Particle

Background: Quercetin is the main active ingredient of Xanthoceras sorbifolia Bunge. Traditional compatibility theory of traditional Chinese medicine has typically reported a synergistic interaction among multiple components, while the synergistic effects of nanoemulsion have not been fully clarified. Objective: To study preparation and characterization of quercetin-based Mongolia Medicine Sendeng-4 nanoemulsion (NQUE-NE) and its antibacterial activity and mechanisms. Methods: The morphology of the nanoemulsion was observed by transmission electron microscopy (TEM), and the zeta potential, polydispersity index (PDI), and particle size distribution were determined by the nanometer particle size analyze. The stability of nanoemulsion was investigated by light test, high speed centrifugal test and storage experiment at different temperature. The combined bacteriostatic effect of N-QUE-NE was studied in vitro by double-dilution method and checkerboard dilution method. Results: The appearance of N-QUE-NE was pale yellow, clear and transparent. The nanoemulsion particles were spherical and uniformly distributed under TEM. The PDI was 0.052, the average particle size was 19.6nm, and the Zeta potential was -0.2mV. When quercetin nanoemulsion (QUE-NE) was used in combination with tannin nanoemulsion (TAN-NE) and toosendanin nanoemulsion (TOO-NE), it exhibited a synergistic antibacterial effect. However, the combination of QUE-NE and geniposide nanoemulsion (GEN-NE) exhibited an antagonistic effect. It was revealed that the antibacterial effect was in order of quercetin-tannin-toosendanin nanoemulsion (QUE-TAN-TOO-NE) ＞ quercetin-tannin nanoemulsion (QUE-TANNE) ＞ QUE-NE ＞ quercetin-tannin-toosendanin-geniposide nanoemulsion (QUE-TAN-TOO-GEN-NE). Conclusion: This study explored the preparation and efficacy of N-QUE-NE, and the results showed that quercetin, tannin and toosendanin had satisfactory synergistic antibacterial effects. The antagonistic effect of quercetin and geniposide in nanoemulsion indicated that it is not beneficial to the antibacterial effect of Sendeng-4, and further research needs to be conducted to clarify its antibacterial effect.

Identification and characterization of a kunzeaol synthase from Thapsia garganica: implications for the biosynthesis of the pharmaceutical thapsigargin

2012 ◽  
Vol 448 (2) ◽  
pp. 261-271 ◽  
Author(s):  
Benjamin Pickel ◽  
Damian P. Drew ◽  
Tom Manczak ◽  
Corinna Weitzel ◽  
Henrik T. Simonsen ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Antagonistic Effect ◽  
Farnesyl Diphosphate ◽  
Fragmentation Pattern ◽  
Terpene Synthases ◽  
Engineered Yeast ◽  
Thapsia Garganica ◽  
Identification And Characterization

Thapsigargin is a major terpenoid constituent of Thapsia garganica root. Owing to its potent antagonistic effect on the sarcoplasmic/endoplasmic reticulum Ca2+-ATPase, thapsigargin has been widely used to study Ca2+ signalling and is also a potential drug for prostate cancer. Despite its importance, thapsigargin biosynthesis in T. garganica remains unknown. In order to decipher thapsigargin biosynthesis, deep transcript sequencing (454 and Illumina) of the T. garganica root was performed, and two terpene synthases (TgTPS1/2) were identified. Functional characterization of their encoded enzymes in a metabolically engineered yeast revealed that TgTPS1 synthesized δ-cadinene, whereas TgTPS2 produced ten distinct terpenoids. However, cultivation of the TgTPS2-expressing yeast in pH-maintained conditions (pH 6–7) yielded one major oxygenated sesquiterpenoid, suggesting that formation of multiple terpenoids was caused by acidity. The major terpene product from TgTPS2 was identified as 6β-hydroxygermacra-1(10),4-diene (kunzeaol) by mass-fragmentation pattern, retention index, the nature of its acid-induced degradation and NMR. Also, recombinant TgTPS2 efficiently catalysed the synthesis of kunzeaol in vitro from farnesyl diphosphate with a Km of 2.6 μM and a kcat of 0.03 s−1. The present paper is the first report of a kunzeaol synthase, and a mechanism for the transformation of kunzeaol into the thapsigargin backbone is proposed.

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