scholarly journals Effects of adenine sulphate, glutamine and casein hydrolysate on in vitro shoot multiplication and rooting of Kinnow mandarin (Citrus reticulata Blanco)

2012 ◽  
Vol 11 (92) ◽  
pp. 15852-15862 ◽  
Author(s):  
Siwach Priyanka ◽  
Chanana Swati ◽  
Rani Gill Anita ◽  
Dh Poonam ◽  
a ◽  
...  
2017 ◽  
Vol 16 (46) ◽  
pp. 2152-2159
Author(s):  
Silue Oumar ◽  
Modeste Kouassi Kan ◽  
Edmond Koffi Kouablan ◽  
Eugene Pacome Kouakou Konan ◽  
Ake Severin

2020 ◽  
Vol 41 (5) ◽  
pp. 1068-1074
Author(s):  
S. Kumar ◽  
◽  
R. Kumar ◽  
P.C. Sharma ◽  
◽  
...  

2015 ◽  
Vol 14 (15) ◽  
pp. 1290-1296
Author(s):  
Kamruzzaman Mohammad ◽  
Akther Arfa ◽  
Omar Faruq Md ◽  
Pervin Afroza ◽  
Myti Sanat ◽  
...  

1970 ◽  
Vol 19 (2) ◽  
pp. 237-245 ◽  
Author(s):  
Pranay Bantawa ◽  
Olivia Saha Roy ◽  
Parthadeb Ghosh ◽  
Tapan Kumar Mondal

An alternative protocol for in vitro propagation of Picrorhiza scrophulariiflora is described using bavistin and adenine sulphate. The explants differentiated into multiple shoot buds on MS supplemented with various concentrations of bavistin and adenine sulphate ranging from 0 - 400 mg/l either alone or in combination. Maximum number of multiple shoots were obtained on MS containing the combination of bavistin (100 mg/l) and adenine sulphate (100 mg/l). In this combination as high as 28 shoots per explant was achieved and also vetrification of the cultures were not recorded. This study also demonstrates that the bavistin has stronger cytokinin-like activity than adenine sulphate. For instance, it was observed that bavistin alone in the concentration of 300 mg/l produced as high as 24 shoots per explant, however, adenine sulphate (100 mg/l) could produce a maximum of 18 shoots per explant. Moreover, higher or lower concentration did not improve the shoot multiplication. The microshoots were separated from the multiple shoots and transferred to MS containing various concentrations of auxins. Among them, NAA (1 mg/l) produced as high as 6 roots per explant. The regenerated plantlets were hardened in plastic cups (6 x 8 cm) containing 9 : 1 virgin soil and soil at Kyongnosla nursery and acclimated for four weeks. A 90% survival rate of the plants was recorded after 60 days. D.O.I. 10.3329/ptcb.v19i2.5441 Plant Tissue Cult. & Biotech. 19(2): 237-245, 2009 (December)


2017 ◽  
Vol 4 (2) ◽  
pp. 17
Author(s):  
. Karyanti ◽  
Minda Kartini

Effect of Thidiazuron and Casein Hydrolysate on In Vitro Shoot Multiplication of Satoimo (Colocasia esculenta (L.) Schott var antiquorum)ABTRACTSatoimo (Colocasia esculenta (L.) Schott var antiquorum) is an alternative substitute of rice which has a big potential to be developed in Indonesia as an export commodity to Japan. Satoimo production needs to be increased to meet the demands for of the plant seeds. Plant propagation can be done using optimal media to stimulate the formation of shoots through, amongst others, the addition of thidiazuron (TDZ) and casein hydrolysate into the culture medium. This study aimed to determine the optimal concentration of TDZ and casein hydrolysate for in vitro multiplication of satoimo shoots. This research used RAL method with 2 factorials, namely the addition of TDZ at 0; 0.2; 0.6 mg/L concentrations, and of casein hydrolysate at 0; 150; 300; 450 mg/L concentrations. The results showed that the use of 0.6 mg/L TDZ and 150 mg/L casein hydrolysate resulted in the highest number of shoots, with the shoot average number of 6.9 per explant.Keywords: Casein hydrolysate, optimal medium, Satoimo, shoot multiplication, TDZ  ABTRAKSatoimo (Colocasia esculenta (L.) Schott var antiquorum) merupakan salah satu bahan alternatif pengganti beras yang memiliki peluang besar untuk dikembangkan di Indonesia, salah satunya sebagai komoditas ekspor ke negara Jepang. Produksi satoimo perlu ditingkatkan untuk memenuhi kebutuhan bibit tanaman tersebut. Perbanyakan tanaman dapat dilakukan menggunakan media yang optimal untuk merangsang pembentukan tunas, salah satunya dengan penambahan thidiazuron (TDZ) dan hidrolisat kasein pada media tanam. Penelitian ini bertujuan untuk mengetahui konsentrasi TDZ dan hidrolisat kasein yang optimal untuk perbanyakan tunas satoimo secara in vitro. Penelitian ini menggunakan metode RAL dengan 2 faktorial yaitu konsentrasi TDZ yang terdiri dari 0; 0,2; 0,6 mg/L dan konsentrasi hidrolisat kasein yang terdiri dari 0; 150; 300; 450 mg/L. Hasil penelitian menunjukkan bahwa pemberian TDZ 0,6 mg/L dan hidrolisat kasein 150 mg/L menghasilkan jumlah tunas tertinggi, dengan rata-rata tunas yang terbentuk 6,9 per eksplan.Kata kunci: Hidrolisat kasein, multiplikasi tunas, optimasi media, Satoimo, TDZ 


2015 ◽  
Vol 189 ◽  
pp. 208-213 ◽  
Author(s):  
Rakesh Sanabam ◽  
Nandeibam Samarjit Singh ◽  
Pratap Jyoti Handique ◽  
Huidrom Sunitibala Devi

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