Establishment of an efficient callus induction and plant regeneration system in some wheat (Triticum aestivum L.) cultivars grown in Sudan

2012 ◽  
Vol 11 (16) ◽  
Author(s):  
Hala AL. Abdallah
Keyword(s):  
Triticum Aestivum ◽  
Plant Regeneration ◽  
Callus Induction ◽  
Triticum Aestivum L ◽  
Regeneration System

scholarly journals Enhancement of Somatic Embryogenesis by Mature and Immature Seeds in Wheat (Triticum aestivum L.)

2017 ◽  
Vol 8 (2) ◽  
pp. 20 ◽  
Author(s):  
Supria Saha ◽  
Zohorul Islam ◽  
Sadequl Islam ◽  
Mirza Fida Hassan ◽  
Md. Shahadat Hossain ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Triticum Aestivum L ◽  
Ms Medium ◽  
Regeneration System ◽  
Wheat Varieties ◽  
Immature Seeds ◽  
Significant Difference ◽  
Mature Seeds

A suitable plant regeneration system has been established using 3-4 weeks old calli derived from immature and mature seeds of four wheat varieties viz. Pavon 76, Akbar, Barkat, and Kanchan. As plant growth regulators, various auxins (2,4-D, BAP and IAA) either single or in combination were used in MS medium. The variety Pavon 76 showed maximum (72.25%) callus induction and Akbar exhibited the lowest (37.78%) from calli derived from immature seeds. Hormonal effects on callus induction were evaluated and significant results were found in case of genotypes at P <0.01. Out of four genotypes, the highest frequency of plant regeneration was recorded in Pavon 76 (67.00%) and lowest in Kanchan (43.10%) when 1.5 mg/l BAP and 0.5 mg/l IAA was added in the medium. It was observed that Pavon 76 produced highest number of green plants than others. For mature seeds all of the mentioned genotypes showed significant difference with maximum frequency of callusing in Pavon 76 (69.57%) in MS + 2.5 mg/l 2,4-D followed by Kanchan (60.84%), Barkat (52.73%), and Akbar (47.19%). For plant regeneration, Pavon 76 also showed best performance (64.36%) in MS + 2.0 BAP + 1.0 mg/l IAA, using calli derived from mature seeds. The other genotypes Barkat, Kanchan and Akbar exhibited 59.44, 52.71 and 52.32% regeneration in the same medium respectively. Here, the lowest regeneration (40.63%) was found in Akbar. In this case, it was aimed to establish a suitable protocol for in vitro callus induction and regeneration for advance biotechnological research on wheat in Bangladesh.

Substitution analysis of callus induction and plant regeneration from anther culture in wheat (Triticum aestivum L.)

1988 ◽  
Vol 7 (2) ◽  
pp. 127-129 ◽  
Author(s):  
�va Szak�cs ◽  
G�za Kov�cs ◽  
J�nos Pauk ◽  
Be�ta Barnab�s
Keyword(s):  
Triticum Aestivum ◽  
Plant Regeneration ◽  
Anther Culture ◽  
Callus Induction ◽  
Triticum Aestivum L

scholarly journals Induksi Kalus dan Regenerasi Beberapa Genotipe Gandum (Triticum aestivum L.) secara In Vitro

2016 ◽  
Vol 6 (2) ◽  
pp. 57
Author(s):  
Atmitri Sisharmini ◽  
Aniversari Apriana ◽  
Sustiprijatno Sustiprijatno
Keyword(s):  
Triticum Aestivum ◽  
Plant Regeneration ◽  
Callus Induction ◽  
Basal Medium ◽  
Triticum Aestivum L ◽  
Induction Medium ◽  
In Vitro Plant Regeneration ◽  
Regeneration Medium ◽  
Wheat Genotypes

<p>Callus Induction and In Vitro Plant Regeneration of<br />Wheat Genotypes (Triticum aestivum L.). Atmitri<br />Sisharmini, Aniversari Apriana, and Sustiprijatno. Development<br />of a reliable in vitro plant regeneration procedure for<br />wheat is a prerequisite for its improvement by genetic transformation.<br />The purpose of this study was to obtain methods<br />of callus induction and regeneration of wheat genotypes.<br />This experiment was conducted at ICABIOGRAD. Immature<br />embryos from four wheat genotypes, ie Perdix, Naxos Wew,<br />Combi and Fasan were used to induce callus formation and<br />regeneration rate of callus. For the preparation of callus<br />induction medium, MS-L7 basal medium was supplemented<br />with combination of growth regulators 2,4 dichlorophenoxy<br />acetic acid (2,4-D) and 4-amino-3,5,6-trichloropicolinic acid<br />(picloram). While, plant regeneration medium was prepared<br />using MS basal medium supplemented with combination of<br />three growth regulators i.e. IAA, BAP and kinetin. The results<br />showed that genotype, in vitro culture medium and growth<br />regulators played a dominant role in callus induction and<br />plantlet regeneration. All the 4 genotypes responded positively<br />to callus induction, however, variability was observed<br />not only among the genotypes but also within callus<br />induction medium used. The best induction medium was<br />the MS-L7 basal medium supplemented with combination of<br />phytohormon 4 mg/l 2,4-D + 2 mg/l picloram (GIK-3) which<br />showed 100% callus induction frequency. Whereas, the best<br />regeneration medium was shown by MS basal medium with<br />combination of phytohormon 1.5 mg/l BAP dan 0.5 mg/l<br />kinetin (RG3). Regarding plant regeneration, Perdix was the<br />most responsive genotype to be regenerated with regeneration<br />frequency of 57.33%. The successfully acclimatized<br />planlets in greenhouse were obtained from Perdix and<br />Naxos Wew genotypes. These results will potentially facilitate<br />genetic transformation research of wheat in Indonesia.</p>

scholarly journals Effect of Gamma Radiation on Callus Induction and Plant Regeneration of four Wheats Triticum aestivum L. Genotypes

2007 ◽  
Vol 4 (3) ◽  
pp. 363-368
Author(s):  
Baghdad Science Journal
Keyword(s):  
Triticum Aestivum ◽  
Plant Regeneration ◽  
Gamma Radiation ◽  
Callus Induction ◽  
Dry Weight ◽  
Triticum Aestivum L ◽  
Shoot Tips ◽  
Immature Embryos ◽  
Control Treatment ◽  
Fresh Weight

The effect of gamma radiation at the doses (0 , 5 , 10 or 15 ) Gray on the callus of four Triticum aestivum immature embryos genotypes (AL-Hashmiya , AL-Noor AL-Zahraa and AL-Mellad ) were studied . The fresh and dry weight for callus and shoot tips beside numbers and lengths of the shoots were used as indicators after 8 weeks . Results revealed that (AL-Noor and AL-Zahraa ) was superior by giving highest fresh and dry weight reached 274.2 and 269.2 mg and 26 and 24.3 mg respectively as compared with AL-Hashmiya and AL-Mellad. Moreover, the control treatment and the dose 10 Gray gave highest fresh weight reached 277.4 and 259.1 mg while the dry weight was highest in the control treatment and the dose 5 Gray. addition 10 Gray dose was superior to give highest rate for the number , length , fresh and dry weight of shoots reached 9.7 shoots , 3.6 cm length , 410.2 and 47.9 mg on respectively.

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