Auxin and sugar effects on callus induction and plant regeneration frequencies from mature embryos of wheat (Triticum aestivum L.)

2002 ◽  
Vol 38 (1) ◽  
pp. 39-45 ◽  
Author(s):  
Maria G. Mendoza ◽  
Heidi F. Kaeppler
Keyword(s):  
Triticum Aestivum ◽  
Plant Regeneration ◽  
Callus Induction ◽  
Triticum Aestivum L ◽  
Mature Embryos

scholarly journals Callus Induction and Regeneration from Germinating Mature Embryos of Wheat (Triticum aestivum L.)

2021 ◽  
Vol 50 (4) ◽  
pp. 889-896
Author(s):  
Samih M. Tamimi ◽  
Halima Othman
Keyword(s):  
Triticum Aestivum ◽  
Callus Induction ◽  
High Performance ◽  
Triticum Aestivum L ◽  
Mature Embryo ◽  
Callus Cultures ◽  
Differentiation Potential ◽  
Mature Embryos ◽  
The Mean ◽  
Wheat Callus

A high-performance protocol for callus induction was devised using germinating mature embryos of two local wheat (Triticum aestivum L.) landraces as explant. The results showed that callus development from germinating embryos was rapid starting one day after culture with an induction rate 20 to 25% higher than those of soaked embryos. In addition, the mean rate of growth of callus developed from germinating embryos was 60 to 70% higher than those cultured from soaked embryos. This study also demonstrated a higher frequency of green spots formation(48 to 56%)on callus derived from germinating embryos compared to their soaked counterpart (24 to 28%), suggesting a better differentiation potential of callus cultures derived from germinating embryo. These findings indicate that germinating mature embryo is more suitable explant for wheat callus induction and regeneration than the soaked mature embryo commonly employed for wheat callus culture.

scholarly journals Effects of NaCl Stress on Callus Proliferation and Plant Regeneration from Mature Embryos of Bread Wheat (Triticum aestivum L.) Cultivars Mahon Demias and Hidhab

1970 ◽  
Vol 17 (1) ◽  
pp. 19-27 ◽  
Author(s):  
L Benderradji ◽  
H Bouzerzou ◽  
A Djekoun ◽  
A Benmahammed
Keyword(s):  
Triticum Aestivum ◽  
Bread Wheat ◽  
Salinity Stress ◽  
Callus Induction ◽  
Selection Process ◽  
Triticum Aestivum L ◽  
Nacl Stress ◽  
Mature Embryos ◽  
Callus Proliferation

An in vitro experiment with two cultivars, Mahon Demias and Hidhab of bread wheat (Triticum aestivum L.) exhibited appreciable callus induction but differed significantly in the capacity of calli proliferation and regeneration under salinity stress; even though Mahon Demias appeared relatively more tolerant than Hidhab. The results indicated the need to optimize a robust protocol for callus induction, maintenance and regeneration before selection process for tolerance to salinity is embarked upon.Key words: Bread wheat, Salinity stress, Callus proliferation, Mature embryoDOI = 10.3329/ptcb.v17i1.1117Plant Tissue Cult. & Biotech. 17(1): 19-27, 2007 (June)

Substitution analysis of callus induction and plant regeneration from anther culture in wheat (Triticum aestivum L.)

1988 ◽  
Vol 7 (2) ◽  
pp. 127-129 ◽  
Author(s):  
�va Szak�cs ◽  
G�za Kov�cs ◽  
J�nos Pauk ◽  
Be�ta Barnab�s
Keyword(s):  
Triticum Aestivum ◽  
Plant Regeneration ◽  
Anther Culture ◽  
Callus Induction ◽  
Triticum Aestivum L

scholarly journals Induksi Kalus dan Regenerasi Beberapa Genotipe Gandum (Triticum aestivum L.) secara In Vitro

2016 ◽  
Vol 6 (2) ◽  
pp. 57
Author(s):  
Atmitri Sisharmini ◽  
Aniversari Apriana ◽  
Sustiprijatno Sustiprijatno
Keyword(s):  
Triticum Aestivum ◽  
Plant Regeneration ◽  
Callus Induction ◽  
Basal Medium ◽  
Triticum Aestivum L ◽  
Induction Medium ◽  
In Vitro Plant Regeneration ◽  
Regeneration Medium ◽  
Wheat Genotypes

<p>Callus Induction and In Vitro Plant Regeneration of<br />Wheat Genotypes (Triticum aestivum L.). Atmitri<br />Sisharmini, Aniversari Apriana, and Sustiprijatno. Development<br />of a reliable in vitro plant regeneration procedure for<br />wheat is a prerequisite for its improvement by genetic transformation.<br />The purpose of this study was to obtain methods<br />of callus induction and regeneration of wheat genotypes.<br />This experiment was conducted at ICABIOGRAD. Immature<br />embryos from four wheat genotypes, ie Perdix, Naxos Wew,<br />Combi and Fasan were used to induce callus formation and<br />regeneration rate of callus. For the preparation of callus<br />induction medium, MS-L7 basal medium was supplemented<br />with combination of growth regulators 2,4 dichlorophenoxy<br />acetic acid (2,4-D) and 4-amino-3,5,6-trichloropicolinic acid<br />(picloram). While, plant regeneration medium was prepared<br />using MS basal medium supplemented with combination of<br />three growth regulators i.e. IAA, BAP and kinetin. The results<br />showed that genotype, in vitro culture medium and growth<br />regulators played a dominant role in callus induction and<br />plantlet regeneration. All the 4 genotypes responded positively<br />to callus induction, however, variability was observed<br />not only among the genotypes but also within callus<br />induction medium used. The best induction medium was<br />the MS-L7 basal medium supplemented with combination of<br />phytohormon 4 mg/l 2,4-D + 2 mg/l picloram (GIK-3) which<br />showed 100% callus induction frequency. Whereas, the best<br />regeneration medium was shown by MS basal medium with<br />combination of phytohormon 1.5 mg/l BAP dan 0.5 mg/l<br />kinetin (RG3). Regarding plant regeneration, Perdix was the<br />most responsive genotype to be regenerated with regeneration<br />frequency of 57.33%. The successfully acclimatized<br />planlets in greenhouse were obtained from Perdix and<br />Naxos Wew genotypes. These results will potentially facilitate<br />genetic transformation research of wheat in Indonesia.</p>

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