scholarly journals Further improvement of N6 medium for callus induction and plant regeneration from maize immature embryos

2011 ◽  
Vol 10 (14) ◽  
pp. 2618-2624 ◽  
Author(s):  
Fu Feng Ling ◽  
He Jing ◽  
Zhang Zhi Yong ◽  
Zhou Shu Feng ◽  
Zhang Su Zhi ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Immature Embryos ◽  
N6 Medium

scholarly journals Regenerasi dan Aklimatisasi Kultur Antera Enam Persilangan F1 Padi Sawah

2016 ◽  
Vol 44 (2) ◽  
pp. 133
Author(s):  
Cucu Gunarsih ◽  
Bambang Sapta Purwoko ◽  
Iswari Saraswati Dewi ◽  
Dan Muhamad Syukur
Keyword(s):  
Plant Regeneration ◽  
Anther Culture ◽  
Doubled Haploid ◽  
Callus Induction ◽  
Rainfed Rice ◽  
Randomized Design ◽  
Doubled Haploid Plants ◽  
Completely Randomized Design ◽  
N6 Medium ◽  
Haploid Plants

ABSTRACT<br /><br />The breeding of rainfed rice tolerant to drought can be accomplished using anther culture. The objectives of this research were to determine regeneration abilities of six F1 anther culture and its acclimatization ability. The experiment was arranged in completely randomized design with 14 replications. The treatments consisted of six F1 derived from crossing:  INPARI 18 x IR83140-B-11-B (G1), INPARI 18 x B12825E-TB-1-25 (G2), INPARI 18 x IR87705-14-11-B-SKI-12 (G3), INPARI 22 x IR83140-B-11-B (G4), Bio-R81 x O18b-1 (G5), Bio-R82-2 x O18b-1 (G6). Media for callus induction was based on N6 medium + 2.0 mg L-1 NAA + 0.5 mg L-1 kinetin + 1.0 mM putresin + 60 g L-1 sucrosa, media for regeneration was based on MS + 0.5 mg L-1 NAA + 2.0 mg L-1 kinetin + 1.0 mM  putresin, and media for rooting was based on  MS + 0.5 mg L-1 IBA + 30 g L-1 sucrosa. The result indicated that all six F1 had different ability in anther culture. Bio-R82-2 x O18-b1 (G6) and  Bio-R81 x O18-b1 (G5) F1 genotype had good response both of callus induction and plant regeneration. These two F1 genotypes also gave the highest ratio of green planlet production to number of anther inoculated (GP:AI) were 5.50% and 4.65%,  respectively. In this research, there were identified doubled haploid plants were developed from 4 F1 derived cross namely G2 (2 plants), G3 (4 plants),  G5 (21 plants), and G6 (26 plants).<br /><br />Keywords: Callus induction, doubled haploid, rice<br /><br />

Plant regeneration through callus initiation from mature and immature embryos of maize (Zea mays L.)

2016 ◽  
Author(s):  
M. Guruprasad ◽  
V. Sridevi ◽  
G. Vijayakumar ◽  
M. Satish Kumar
Keyword(s):  
Zea Mays ◽  
Plant Regeneration ◽  
Zea Mays L ◽  
Immature Embryos ◽  
Embryogenic Calli ◽  
Regeneration Time ◽  
Mature Embryos ◽  
Induction Frequency ◽  
Efficient Regeneration ◽  
N6 Medium

An efficient regeneration was developed using mature and immature embryos by using Maize (Zea mays L) variety MU 2092. Mature embryos are removed from surface sterilized seeds, slice them into halves and immature embryos are detached from seed endosperm. Both are used as explants to initiate callus on N6 medium supplemented with 2,4 D @ 4.0 mg.L-1. The induction frequency of primary calli i.e embryogenic callus was 90% in maize. The embryogenic calli on N6 medium supplemented with 6-benzylaminopurine (BAP) @ 0.5 mg.L-1 and kinetin @ 0.5 mg.L-1 was more effective in producing shoots. The culture expressed maximum plant regeneration potential with eight shoots per embryo on regeneration. Green shoots thus developed were successfully rooted within 20 days on MS media containing IBA (Indole-3-Butyric acid) 1mg L-1. Over 86 % of rooted plants grew well and produced seeds normally when transferred to green house. The important advantage of this improved method is shortening of regeneration time by providing an efficient and rapid regeneration tool for mature and immature embryos.

scholarly journals Optimization of Callus Induction Conditions from Immature Embryos in Maize and Plant Regeneration

Phyton ◽  
2020 ◽  
Vol 89 (1) ◽  
pp. 121-130
Author(s):  
Peng Jiao ◽  
Ruiqi Ma ◽  
Zhuo Qi ◽  
Zhenzhong Jiang ◽  
Siyan Liu ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Immature Embryos

scholarly journals Callus induction and plant regeneration by Brazilian triticale and wheat genotypes

1997 ◽  
Vol 20 (1) ◽  
pp. 41-44 ◽  
Author(s):  
A.L.C. Dornelles ◽  
F.I.F. Carvalho ◽  
L.C. Federizzi ◽  
C.L. Handel ◽  
F. Bered ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Culture Media ◽  
Rio Grande ◽  
Immature Embryos ◽  
Ms Medium ◽  
Rio Grande Do Sul ◽  
Wheat Genotypes ◽  
The One

In order to determine the in vitro behavior of Brazilian triticale, 16 triticale genotypes, and three wheat genotypes used as checks, were sown in June 1994. The explants used were immature embryos. In addition to the genotype tests, two culture media for callus induction were also evaluated, i.e., MS (Murashige and Skoog, Physiol. Plant. 15: 473-497, 1962) medium containing 2.0 mg 2,4D/l, and MS medium containing 4.0 mg 2,4D/l. The plant regeneration protocol used was the one employed at the Laboratório de Cultura de Tecidos, Departamento de Plantas de Lavoura, Universidade Federal do Rio Grande do Sul, for wheat. Differences in plant regeneration were observed both among triticale and wheat genotypes, with triticale usually showing better regeneration than wheat. No differences were observed between the callus induction media.

scholarly journals Effect of Genotype and Culture Media on Callus Induction and Plant Regeneration from Matured Rice Grain Culture

2005 ◽  
Vol 26 ◽  
pp. 21-26 ◽  
Author(s):  
RK Niroula ◽  
BP Sah ◽  
HP Bimb ◽  
S Nayak
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Culture Media ◽  
In Vitro Regeneration ◽  
Rice Grain ◽  
Mineral Salts ◽  
Organic Salts ◽  
Rice Genotypes ◽  
N6 Medium

This study was under taken to elucidate the effect of genotypes and media compositions on callus induction from mature rice seeds. Three different callus induction media, designated as A (N6 mineral salts + N6 vitamins, 2 mg/l each + myoinositol, 100 mg/l + 2,4-D, 2.5 mg/l + kinetin, 0.5mg/l + AgNO3, 10 mg/l + maltose, 50 gm/l); B (MS organic salts + N6 mineral salts + NAA, 4 mg/l + kinetin, 2 mg/l + AgNO3, 5mg/l and sucrose, 60 gm/l); and C (B media without AgNO3), and six rice genotypes viz. Jumlimarshi, Tilki, Jethobudo, Manshara, Masuli and Pahenle were evaluated. The modified N6 medium supplemented with 2, 4-D, 2.5 mg/l and AgNO3, 10 mg/l exhibited better performance in callus induction. Among genotypes, callus induction frequency was higher (100%) in Masuli, Tilki and Jumlimarshi regardless of media tested. The positive effect of AgNO3 was only observed in medium A for quality callus induction and subsequent plant regeneration. The genotype Tilki performed better regarding plant regeneration (27.77%). Therefore, it is suggested that application of medium A is advantageous to accomplish overall efficiency of callus induction and plant regeneration from seeds of various rice genotypes. Key words: 2, 4-D; AgNO3; dehulled rice; in vitro; regeneration J. Inst. Agric. Anim. Sci. 26:21-26 (2005)

scholarly journals Effect of Gamma Radiation on Callus Induction and Plant Regeneration of four Wheats Triticum aestivum L. Genotypes

2007 ◽  
Vol 4 (3) ◽  
pp. 363-368
Author(s):  
Baghdad Science Journal
Keyword(s):  
Triticum Aestivum ◽  
Plant Regeneration ◽  
Gamma Radiation ◽  
Callus Induction ◽  
Dry Weight ◽  
Triticum Aestivum L ◽  
Shoot Tips ◽  
Immature Embryos ◽  
Control Treatment ◽  
Fresh Weight

The effect of gamma radiation at the doses (0 , 5 , 10 or 15 ) Gray on the callus of four Triticum aestivum immature embryos genotypes (AL-Hashmiya , AL-Noor AL-Zahraa and AL-Mellad ) were studied . The fresh and dry weight for callus and shoot tips beside numbers and lengths of the shoots were used as indicators after 8 weeks . Results revealed that (AL-Noor and AL-Zahraa ) was superior by giving highest fresh and dry weight reached 274.2 and 269.2 mg and 26 and 24.3 mg respectively as compared with AL-Hashmiya and AL-Mellad. Moreover, the control treatment and the dose 10 Gray gave highest fresh weight reached 277.4 and 259.1 mg while the dry weight was highest in the control treatment and the dose 5 Gray. addition 10 Gray dose was superior to give highest rate for the number , length , fresh and dry weight of shoots reached 9.7 shoots , 3.6 cm length , 410.2 and 47.9 mg on respectively.

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