Callus induction and plant regeneration from immature embryos of rye and triticale

1981 ◽  
Vol 1 (1) ◽  
pp. 221-227 ◽  
Author(s):  
Susan Eapen ◽  
P. S. Rao
2011 ◽  
Vol 10 (14) ◽  
pp. 2618-2624 ◽  
Author(s):  
Fu Feng Ling ◽  
He Jing ◽  
Zhang Zhi Yong ◽  
Zhou Shu Feng ◽  
Zhang Su Zhi ◽  
...  

Phyton ◽  
2020 ◽  
Vol 89 (1) ◽  
pp. 121-130
Author(s):  
Peng Jiao ◽  
Ruiqi Ma ◽  
Zhuo Qi ◽  
Zhenzhong Jiang ◽  
Siyan Liu ◽  
...  

1997 ◽  
Vol 20 (1) ◽  
pp. 41-44 ◽  
Author(s):  
A.L.C. Dornelles ◽  
F.I.F. Carvalho ◽  
L.C. Federizzi ◽  
C.L. Handel ◽  
F. Bered ◽  
...  

In order to determine the in vitro behavior of Brazilian triticale, 16 triticale genotypes, and three wheat genotypes used as checks, were sown in June 1994. The explants used were immature embryos. In addition to the genotype tests, two culture media for callus induction were also evaluated, i.e., MS (Murashige and Skoog, Physiol. Plant. 15: 473-497, 1962) medium containing 2.0 mg 2,4D/l, and MS medium containing 4.0 mg 2,4D/l. The plant regeneration protocol used was the one employed at the Laboratório de Cultura de Tecidos, Departamento de Plantas de Lavoura, Universidade Federal do Rio Grande do Sul, for wheat. Differences in plant regeneration were observed both among triticale and wheat genotypes, with triticale usually showing better regeneration than wheat. No differences were observed between the callus induction media.


2007 ◽  
Vol 4 (3) ◽  
pp. 363-368
Author(s):  
Baghdad Science Journal

The effect of gamma radiation at the doses (0 , 5 , 10 or 15 ) Gray on the callus of four Triticum aestivum immature embryos genotypes (AL-Hashmiya , AL-Noor AL-Zahraa and AL-Mellad ) were studied . The fresh and dry weight for callus and shoot tips beside numbers and lengths of the shoots were used as indicators after 8 weeks . Results revealed that (AL-Noor and AL-Zahraa ) was superior by giving highest fresh and dry weight reached 274.2 and 269.2 mg and 26 and 24.3 mg respectively as compared with AL-Hashmiya and AL-Mellad. Moreover, the control treatment and the dose 10 Gray gave highest fresh weight reached 277.4 and 259.1 mg while the dry weight was highest in the control treatment and the dose 5 Gray. addition 10 Gray dose was superior to give highest rate for the number , length , fresh and dry weight of shoots reached 9.7 shoots , 3.6 cm length , 410.2 and 47.9 mg on respectively.


2014 ◽  
Vol 49 (5) ◽  
pp. 595 ◽  
Author(s):  
Feng Huan ◽  
Yi Shuli ◽  
Xie Jiaheng ◽  
Lei Mengqi ◽  
Huang Xuan

2021 ◽  
Author(s):  
Chenchen Wang ◽  
Haizhen Ma ◽  
Weiwei Zhu ◽  
Jiedao Zhang ◽  
Xiangyu Zhao ◽  
...  

2015 ◽  
Vol 25 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Kee Hwa Bae ◽  
Eui Soo Yoon

Lychnis cognate Maxim and Lychnis fulgens Fish. Ex Spreng are two valued ornamental plants in Korea. Soaking of seeds in GA3 solution remarkably promoted germination up to 60%, but the control (0 mg/l) was not effective (> 5%). To select an adequate temperature for seed germination, seeds, previously soaked in a 1000 mg/l GA3 for 24 hrs, were incubated at 15, 20, 25, and 30°C. Seed germination of over 20% was obtained at 15, 20, and 25°C, but only 10% at 30°C. These results indicate that the seeds of L. cognate and L. fulgens are in a such dormant state that they hardly germinate even by dormancy breaker (GA3) and low (15 ? 25°C) temperature treatment. The highest callus induction was observed in the leaf explants of the seedlings on MS containing specific concentrations of 3.0 mg/l BA and 1.0 mg/l NAA. The adventitious shoot was formed < 90% of calli on 1/2 WPM medium. The height of in vitro propagated plantlet was no different media used for regeneration. This in vitro propagation protocol should be useful for conservation of endangered and ornamental plant.Plant Tissue Cult. & Biotech. 25(1): 1-12, 2015 (June)


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