Determination of Virulence Factors in Clinical Multidrug Resistance Enterococci Isolates at Southeast of Iran

2017 ◽  
Vol 10 (5) ◽  
Author(s):  
Shahram Shahraki ◽  
Morteza Rabi Nezhad Mousavi
Keyword(s):  
Multidrug Resistance ◽  
Virulence Factors

The Determination of Virulence Factors among Fish Originated Enterococci

2016 ◽  
Vol 27 (2) ◽  
pp. 98-103 ◽  
Author(s):  
Serap SAVAŞAN ◽  
Şükrü KIRKAN ◽  
Göksel ERBAŞ ◽  
Uğur PARIN ◽  
Alper ÇİFTCİ
Keyword(s):  
Virulence Factors

scholarly journals Phosphorylation of MgrA and Its Effect on Expression of the NorA and NorB Efflux Pumps of Staphylococcus aureus

2010 ◽  
Vol 192 (10) ◽  
pp. 2525-2534 ◽  
Author(s):  
Que Chi Truong-Bolduc ◽  
David C. Hooper
Keyword(s):  
Staphylococcus Aureus ◽  
Multidrug Resistance ◽  
Virulence Factors ◽  
Double Mutant ◽  
Efflux Pumps ◽  
Efflux Transporters ◽  
Global Regulator ◽  
Binding Assays ◽  
Genes Encoding ◽  
Gel Shift

ABSTRACT MgrA is a global regulator in Staphylococcus aureus that controls the expression of diverse genes encoding virulence factors and multidrug resistance (MDR) efflux transporters. We identified pknB, which encodes the (Ser/Thr) kinase PknB, in the S. aureus genome. PknB was able to autophosphorylate as well as phosphorylate purified MgrA. We demonstrated that rsbU, which encodes a Ser/Thr phosphatase and is involved in the activation of the SigB regulon, was able to dephosphorylate MgrA-P but not PknB-P. Serines 110 and 113 of MgrA were found to be phosphorylated, and Ala substitutions at these positions resulted in reductions in the level of phosphorylation of MgrA. DNA gel shift binding assays using norA and norB promoters showed that MgrA-P was able to bind the norB promoter but not the norA promoter, a pattern which was the reverse of that for unphosphorylated MgrA. The double mutant MgrAS110A-S113A bound to the norA promoter but not the norB promoter. The double mutant led to a 2-fold decrease in norA transcripts and a 2-fold decrease in the MICs of norfloxacin and ciprofloxacin in strain RN6390. Thus, phosphorylation of MgrA results in loss of binding to the norA promoter, but with a gain of the ability to bind the norB promoter. Loss of the ability to phosphorylate MgrA by Ala substitution resulted in increased repression of norA expression and in reductions in susceptibilities to NorA substrates.

scholarly journals Escherichia coli with virulence factors and multidrug resistance in the Plankenburg River

2014 ◽  
Vol 110 (9/10) ◽  
pp. 1-6 ◽  
Author(s):  
Corne Lamprecht ◽  
Marco Romanis ◽  
Nicola Huisamen ◽  
Anneri Carinus ◽  
Nika Schoeman ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistance ◽  
Virulence Factors

scholarly journals Acquisition of multidrug resistance transposon Tn6061 and IS6100-mediated large chromosomal inversions in Pseudomonas aeruginosa clinical isolates

Microbiology ◽  
2010 ◽  
Vol 156 (5) ◽  
pp. 1448-1458 ◽  
Author(s):  
Sébastien Coyne ◽  
Patrice Courvalin ◽  
Marc Galimand
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Multidrug Resistance ◽  
Opportunistic Pathogen ◽  
Clinical Isolates ◽  
Chromosomal Inversions ◽  
Gene Acquisition ◽  
Drug Classes ◽  
Horizontal Acquisition

Pseudomonas aeruginosa is a major human opportunistic pathogen, especially for patients in intensive care units or with cystic fibrosis. Multidrug resistance is a common feature of this species. In a previous study we detected the ant(4′)-IIb gene in six multiresistant clinical isolates of P. aeruginosa, and determination of the environment of the gene led to characterization of Tn6061. This 26 586 bp element, a member of the Tn3 family of transposons, carried 10 genes conferring resistance to six drug classes. The ant(4′)-IIb sequence was flanked by directly repeated copies of ISCR6 in all but one of the strains studied, consistent with ISCR6-mediated gene acquisition. Tn6061 was chromosomally located in six strains and plasmid-borne in the remaining isolate, suggesting horizontal acquisition. Duplication-insertion of IS6100, that ended Tn6061, was responsible for large chromosomal inversions. Acquisition of Tn6061 and chromosomal inversions are further examples of intricate mechanisms that contribute to the genome plasticity of P. aeruginosa.

Isolation and Identification of Fungal Isolates caused Otomycosis from some Hospitals and Clinics in Mosul with Determination of Their Virulence Factors

2021 ◽  
Vol 26 (4) ◽  
Author(s):  
Nabeel Al-Sharrad ◽  
Muhammad A. Al-Kataan ◽  
Maha A. Al-Rejaboo
Keyword(s):  
Fungal Infection ◽  
Virulence Factors ◽  
Biofilm Formation ◽  
Fungal Pathogens ◽  
Candida Parapsilosis ◽  
Isolation And Identification ◽  
Fungal Isolates ◽  
Biofilm Production ◽  
Etiological Agents

Otomycosis is a fungal infection that frequently involves the external auditory canal. In this study, we aimed to isolation and identification the fungal isolates as etiological agents of otomycosis from some hospitals and clinics in Mosul with determination of their virulence factors of fungal etiological agents. Positive fungal infection was found in (43) samples (71.6%). The most common fungal pathogens were Candida and Aspergillus species, with Candida parapsilosis being the predominant isolates in (11) samples (16.6%). Otomycosis was more common in Female in (26) samples (43.3%).Otomycosis was the highest prevalence aged group 15-40 years (19) samples (31.3%). The present study of virulence factors revealed that the highest biofilm formation isolates were C. parapsilosis is (10) isolates which were distributed between (2) strong and (8) weak biofilm formation.Where C.trpicales, was recorded as least isolates for biofilm production.

scholarly journals The antimicrobial susceptibility and virulence factors of Bacillus anthracis strains isolated in Croatia

2011 ◽  
Vol 56 (No. 1) ◽  
pp. 23-28 ◽  
Author(s):  
B. Habrun ◽  
I. Racic ◽  
G. Kompes ◽  
S. Spicic ◽  
M. Benic ◽  
...  
Keyword(s):  
United States ◽  
Bacillus Anthracis ◽  
Clavulanic Acid ◽  
Virulence Factors ◽  
Antimicrobial Susceptibility ◽  
The United States ◽  
Antimicrobial Substances

Bacillus anthracis can infect both livestock and humans. The importance of the treatment of the disease in humans has been underscored by the bioterrorism events of 2001 in the United States. The presence of PA and B/C genes (pX01 and pX02 plasmids) as well as susceptibility to several antimicrobial substances was determined in 11 strains of Bacillus anthracis isolated during two recent epizooties of anthrax which occurred in Croatia in 2002 among sheep and in 2006/2007 in cattle. The pX01 plasmid was observed in all of the examined strains, including vaccinal Sterne strains. However, the pX02 plasmid was detected in only eight out of eleven examined field strains of Bacillus anthracis while in vaccinal strains it was not detected at. Determination of MIC's revealed susceptibility to amoxicillin, amoxicillin with clavulanic acid, ciprofloxacin, gentamicin and tetracycline. All strains were resistant to sulfamethoxazole with trimethoprim and cefotaxime.

Flow cytometry evaluation of the multidrug resistance phenotype with the use of C 219, JSB1 and MRK16 monoclonal anbitodies, daunorubicin uptake and determination of glutatione level

1992 ◽  
Vol 76 (2) ◽  
pp. 237-237
Author(s):  
Lizard Gérard ◽  
Roignot Patrick ◽  
Maynadie Marc ◽  
Dusserre-Guion Laurence ◽  
Morlevat Fabienne ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Multidrug Resistance ◽  
Resistance Phenotype
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