The Determination of Virulence Factors among Fish Originated Enterococci

2016 ◽  
Vol 27 (2) ◽  
pp. 98-103 ◽  
Author(s):  
Serap SAVAŞAN ◽  
Şükrü KIRKAN ◽  
Göksel ERBAŞ ◽  
Uğur PARIN ◽  
Alper ÇİFTCİ
Keyword(s):  
2021 ◽  
Vol 26 (4) ◽  
Author(s):  
Nabeel Al-Sharrad ◽  
Muhammad A. Al-Kataan ◽  
Maha A. Al-Rejaboo

Otomycosis is a fungal infection that frequently involves the external auditory canal. In this study, we aimed to isolation and identification the fungal isolates as etiological agents of otomycosis from some hospitals and clinics in Mosul with determination of their virulence factors of fungal etiological agents. Positive fungal infection was found in (43) samples (71.6%). The most common fungal pathogens were Candida and Aspergillus species, with Candida parapsilosis being the predominant isolates in (11) samples (16.6%). Otomycosis was more common in Female in (26) samples (43.3%).Otomycosis was the highest prevalence aged group 15-40 years (19) samples (31.3%). The present study of virulence factors revealed that the highest biofilm formation isolates were C. parapsilosis is (10) isolates which were distributed between (2) strong and (8) weak biofilm formation.Where C.trpicales, was recorded as least isolates for biofilm production.


2011 ◽  
Vol 56 (No. 1) ◽  
pp. 23-28 ◽  
Author(s):  
B. Habrun ◽  
I. Racic ◽  
G. Kompes ◽  
S. Spicic ◽  
M. Benic ◽  
...  

Bacillus anthracis can infect both livestock and humans. The importance of the treatment of the disease in humans has been underscored by the bioterrorism events of 2001 in the United States. The presence of PA and B/C genes (pX01 and pX02 plasmids) as well as susceptibility to several antimicrobial substances was determined in 11 strains of Bacillus anthracis isolated during two recent epizooties of anthrax which occurred in Croatia in 2002 among sheep and in 2006/2007 in cattle. The pX01 plasmid was observed in all of the examined strains, including vaccinal Sterne strains. However, the pX02 plasmid was detected in only eight out of eleven examined field strains of Bacillus anthracis while in vaccinal strains it was not detected at. Determination of MIC's revealed susceptibility to amoxicillin, amoxicillin with clavulanic acid, ciprofloxacin, gentamicin and tetracycline. All strains were resistant to sulfamethoxazole with trimethoprim and cefotaxime.


Author(s):  
Maryam Shafigh ◽  
Fariba Akrami ◽  
Amirmorteza Ebrahimzadeh Namvar

Background and Aims: Uropathogenic E.coli is one of the most known causes of urinary tract infections, which may lead to a high rate of morbidity and mortality in high risk patients. In this regard, the virulence factors such as bacterial adhesion molecules have a critical role. The current study was intended to determine the molecular properties of adhesion genes in Uropathogenic E.coli strains isolated from hospitalized patients in Babol, north of Iran. Materials and Methods: During a nine-months of study, 90 Uropathogenic E.coli strains were confirmed by differential biochemical and microbiological standard tests, antimicrobial susceptibility test, and molecular PCR assay were obtained for evaluating the frequency of adhesion genes. Results: According to the results, the highest rate of resistance and susceptibility were belonged to penicillin and imipenem respectively. On the other hand, the percentage of fimH gene among other virulence genes was reported to be 66%. Conclusions: The present study showed that a high level of Uropathogenic E.coli isolates which harbored the adhesion factors may lead to distribution of multiple antimicrobial resistance strains.


2007 ◽  
Vol 53 (3) ◽  
pp. 372-379 ◽  
Author(s):  
N. Klibi ◽  
K. Ben Slama ◽  
Y. Sáenz ◽  
A. Masmoudi ◽  
S. Zanetti ◽  
...  

Phenotypic and genotypic determination of virulence factors were carried out in 46 high-level gentamicin-resistant (HLGR) clinical Enterococcus faecalis (n = 34) and Enterococcus faecium (n = 12) isolates recovered from different patients in La Rabta Hospital in Tunis, Tunisia, between 2000 and 2003 (all these isolates harboured the aac(6′)–aph(2″) gene). The genes encoding virulence factors (agg, gelE, ace, cylLLS, esp, cpd, and fsrB) were analysed by PCR and sequencing. The production of gelatinase and hemolysin, the adherence to caco-2 and hep-2 cells, and the capacity for biofilm formation were investigated in all 46 HLGR enterococci. The percentages of E. faecalis isolates harbouring virulence genes were as follows: gelE, cpd, and ace (100%); fsrB (62%); agg (56%); cylLLS (41.2%); and esp (26.5%). The only virulence gene detected among the 12 HLGR E. faecium isolates was esp (58%). Gelatinase activity was detected in 22 of the 34 E. faecalis isolates (65%, most of them with the gelE+–fsrB+ genotype); the remaining 12 isolates were gelatinase-negative (with the gelE+–fsrB– genotype and the deletion of a 23.9 kb fragment of the fsr locus). Overall, 64% of the cylLLS-containing E. faecalis isolates showed β-hemolysis. A high proportion of our HLGR E. faecalis isolates, in contrast to E. faecium, showed moderate or strong biofilm formation or adherence to caco-2 and hep-2 cells.


2001 ◽  
Vol 18 (5) ◽  
pp. 479-488 ◽  
Author(s):  
W. Baffone ◽  
B. Citterio ◽  
E. Vittoria ◽  
A. Casaroli ◽  
A. Pianetti ◽  
...  

2016 ◽  
Vol 62 (2) ◽  
pp. 139-144 ◽  
Author(s):  
Fernanda Morcatti Coura ◽  
Soraia de Araújo Diniz ◽  
Jamili Maria Suhet Mussi ◽  
Marcos Xavier Silva ◽  
Andrey Pereira Lage ◽  
...  

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