scholarly journals Detection of human papillomavirus DNA in the various uterocervical lesions by dot blot and southern blot hybridization.

1991 ◽  
Vol 30 (1) ◽  
pp. 28-34
Author(s):  
Hiroshi TANAKA ◽  
Tamikazu TAZAKI ◽  
Kuniko TAKAMURA ◽  
Yasuyuki HASUO ◽  
Keizou FUJIYOSHI ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Southern Blot ◽  
Blot Hybridization ◽  
Southern Blot Hybridization ◽  
Dot Blot ◽  
Papillomavirus Dna

scholarly journals Human papillomavirus in laryngeal papilloma detected by dot blot hybridization (Vira Pap) and southern blot hybridization.

1990 ◽  
Vol 83 (6) ◽  
pp. 909-913
Author(s):  
Shuichi Watanabe ◽  
Yoshihiro Naito ◽  
Takashi Kawakami ◽  
Yu Masuda ◽  
Hajime Ogura ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Southern Blot ◽  
Blot Hybridization ◽  
Southern Blot Hybridization ◽  
Dot Blot ◽  
Laryngeal Papilloma ◽  
Dot Blot Hybridization

Human Papillomavirus DNA in Anal CarcinomasComparison of In Situ and Dot Blot Hybridization

1991 ◽  
Vol 96 (3) ◽  
pp. 318-325 ◽  
Author(s):  
MÁire A. Duggan ◽  
Valerie F. Boras ◽  
Masafumi Inoue ◽  
S. Elizabeth McGregor
Keyword(s):  
Human Papillomavirus ◽  
Blot Hybridization ◽  
Dot Blot ◽  
Dot Blot Hybridization ◽  
Papillomavirus Dna

scholarly journals Genetic Transformation of Dendrobium 'Sonia Earsakul' with Antisense Carica papaya ACO1 Gene

2015 ◽  
Vol 9 (12) ◽  
pp. 125 ◽  
Author(s):  
Piyanuch Sornchai ◽  
Sermsiri Chanprame
Keyword(s):  
Enzyme Activity ◽  
Southern Blot ◽  
Ethylene Production ◽  
Blot Hybridization ◽  
Acc Oxidase ◽  
Southern Blot Hybridization ◽  
Plant Genome ◽  
Vase Life ◽  
Dot Blot ◽  
Transgenic Lines

<p><em>Dendrobium</em> orchid is one of the major export cut flowers not only in Thailand but also for several tropical countries. However, the production of ethylene by their flowers causes a shorter vase life. Flowers that contained lower levels of ethylene usually exhibited delayed senescence and consequently prolonged vase life. The transfer of antisense <em>ACC oxidase (ACO)</em> gene into orchid, in theory, may leads to decreased ethylene production because this gene can down regulates the ethylene biosynthesis pathway. This study focuses on the transformation and the existence and expression of the antisense <em>ACO</em>1 gene from papaya, namely (<em>CP-ACO</em>1), which was transferred in to <em>Dendrobium</em> 'Sonia Earsakul'. The successful stable transformation event obtained and the existence of the transferred gene was determined using PCR, dot blot hybridization and Southern blot hybridization techniques. The results revealed that antisense <em>CP-ACO</em>1 and <em>hygromycin phosphotransferase (hpt)</em> gene existed in all transgenic lines confirmed by PCR technique. The genomic dot blot confirmed the incorporation of the transgene in transgenic plant genome. Southern blot hybridization revealed the existed of one to four sets of the gene in transgenic lines. The expression of antisense <em>CP-ACO</em>1 gene was analyzed through the level of ACO enzyme activity and ethylene production in transgenic orchid. All of the transgenic lines had lower ACO enzyme activity and lower ethylene production than that of the non-transgenic orchid plants.<strong> </strong></p> <p> </p>

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