Detection and typing of human papillomavirus DNA in the uterine cervix of japanese women by nonradioactive dot blot and southern blot hybridization

1993 ◽  
Vol 9 (1) ◽  
pp. 20-24 ◽  
Author(s):  
Ryo Konno ◽  
Shinji Sato ◽  
Akira Yajima
Keyword(s):  
Human Papillomavirus ◽  
Southern Blot ◽  
Uterine Cervix ◽  
Blot Hybridization ◽  
Southern Blot Hybridization ◽  
Japanese Women ◽  
Dot Blot ◽  
Papillomavirus Dna

scholarly journals Detection of human papillomavirus DNA in the various uterocervical lesions by dot blot and southern blot hybridization.

1991 ◽  
Vol 30 (1) ◽  
pp. 28-34
Author(s):  
Hiroshi TANAKA ◽  
Tamikazu TAZAKI ◽  
Kuniko TAKAMURA ◽  
Yasuyuki HASUO ◽  
Keizou FUJIYOSHI ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Southern Blot ◽  
Blot Hybridization ◽  
Southern Blot Hybridization ◽  
Dot Blot ◽  
Papillomavirus Dna

scholarly journals Human papillomavirus in laryngeal papilloma detected by dot blot hybridization (Vira Pap) and southern blot hybridization.

1990 ◽  
Vol 83 (6) ◽  
pp. 909-913
Author(s):  
Shuichi Watanabe ◽  
Yoshihiro Naito ◽  
Takashi Kawakami ◽  
Yu Masuda ◽  
Hajime Ogura ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Southern Blot ◽  
Blot Hybridization ◽  
Southern Blot Hybridization ◽  
Dot Blot ◽  
Laryngeal Papilloma ◽  
Dot Blot Hybridization

Human papillomavirus genotype as a major determinant of the course of cervical cancer.

1998 ◽  
Vol 16 (8) ◽  
pp. 2613-2619 ◽  
Author(s):  
I Lombard ◽  
A Vincent-Salomon ◽  
P Validire ◽  
B Zafrani ◽  
A de la Rochefordière ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
Uterine Cervix ◽  
Blot Hybridization ◽  
Invasive Cervical Cancer ◽  
Disease Free Survival ◽  
Southern Blot Hybridization ◽  
Tumor Stage ◽  
Hpv Typing ◽  
Associated Tumors

PURPOSE To determine whether the prognosis of invasive cancers of the uterine cervix is related to the type of human papillomavirus (HPV) associated with the tumor. PATIENTS AND METHODS Two hundred ninety-seven patients with invasive cervical cancer were prospectively registered from 1986 to 1994. HPV typing was performed on DNA extracted from frozen tumor specimens by means of Southern blot hybridization (SBH) and polymerase chain reaction (PCR) techniques. The median follow-up was 38 months. RESULTS HPV sequences were detected in 246 patients (83%): 150 patients had HPV16, 31 patients had HPV18, and 14 patients had one of the intermediate-oncogenic-risk HPV types (HPV31, 33, 35, 52, 58). In 51 patients, HPV type remained undetermined, and in 51 patients, no viral sequences were found. No significant associations were observed between virologic data and tumor stage or node status. The 5-year disease-free survival (DFS) rate was 100% for patients with intermediate-risk HPV-associated tumors, 58% for patients with HPV16-positive tumors, and 38% for patients with HPV18-positive tumors (P = .02). In multivariate analysis, patients with HPV18-associated tumors had a relative risk (RR) of death 2.4 times greater (95% confidence interval [CI], 1.29-4.59) than that for patients with HPV16, and 4.4 times greater (95% CI, 3.48-5.32) than that for patients with a tumor associated with a viral type different from HPV16/18. CONCLUSION The prognosis for invasive cancers of the uterine cervix is dependent on the oncogenic potential of the associated HPV type. HPV typing may provide a prognostic indicator for individual patients and is of potential use in defining specific therapies against HPV-harboring tumor cells.

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