scholarly journals Detection and typing of Papillomavirus DNA by in situ hybridization method in punch biopsy specimens of the human uterine cervix: Comparative with Southern blot hybridization method.

1991 ◽  
Vol 30 (6) ◽  
pp. 1055-1062
Author(s):  
Keiji KERA ◽  
Hideaki IWASAKI ◽  
Bin TAKEDA ◽  
Hiroyoshi TAKAMIZAWA ◽  
Humio HORIUCHI ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Southern Blot ◽  
Uterine Cervix ◽  
Blot Hybridization ◽  
Southern Blot Hybridization ◽  
Punch Biopsy ◽  
Hybridization Method ◽  
Biopsy Specimens ◽  
Papillomavirus Dna

scholarly journals Papillomas and Carcinomas Associated with a Papillomavirus in European Harvest Mice (Micromys minutus)

1988 ◽  
Vol 25 (5) ◽  
pp. 356-361 ◽  
Author(s):  
J. P. Sundberg ◽  
M. K. O'Banion ◽  
A. Shima ◽  
C. Knupp ◽  
M. E. Reichmann
Keyword(s):  
Blot Hybridization ◽  
Southern Blot Hybridization ◽  
Skin Lesions ◽  
Sebaceous Carcinoma ◽  
Cutaneous Lesions ◽  
Pulmonary Tumors ◽  
Papillomavirus Dna ◽  
Inverted Papillomas ◽  
Micromys Minutus

Papillomaviruses, group-specific papillomavirus antigens, or extrachromosomal papillomavirus DNA were detected in cutaneous, mucocutaneous, and pulmonary tumors affecting a colony of European harvest mice (Micromys minutus). Skin lesions were classified as acanthomatous hyperplasia, epidermal inclusion cysts. squamous papillomas, inverted papillomas, trichoepitheliomas, and sebaceous carcinomas. Cutaneous horns (hyperkeratotic papillomas) were on mucocutaneous junctions of one animal. One mouse, with a cutaneous sebaceous carcinoma, had multiple pulmonary keratinaceous cysts. Papillomavirus antigens, detected by the avidin-biotin technique, were in 20 of 31 lesions tested. In contrast, by Southern blot hybridization all 28 lesions tested contained papillomavirus DNA. Papillomavirus DNA was demonstrated in two often benign cutaneous lesions by in situ hybridization.

scholarly journals Detection of human papillomavirus DNA in the various uterocervical lesions by dot blot and southern blot hybridization.

1991 ◽  
Vol 30 (1) ◽  
pp. 28-34
Author(s):  
Hiroshi TANAKA ◽  
Tamikazu TAZAKI ◽  
Kuniko TAKAMURA ◽  
Yasuyuki HASUO ◽  
Keizou FUJIYOSHI ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Southern Blot ◽  
Blot Hybridization ◽  
Southern Blot Hybridization ◽  
Dot Blot ◽  
Papillomavirus Dna

Tandem repeat DNA localizing on the proximal DAPI bands of chromosomes in Larix, Pinaceae

Genome ◽  
2002 ◽  
Vol 45 (4) ◽  
pp. 777-783 ◽  
Author(s):  
Masahiro Hizume ◽  
Fukashi Shibata ◽  
Ayako Matsumoto ◽  
Yukie Maruyama ◽  
Eiji Hayashi ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Repetitive Dna ◽  
Genomic Dna ◽  
Repeat Unit ◽  
Blot Hybridization ◽  
Southern Blot Hybridization ◽  
Repeat Sequence ◽  
Proximal Region

Repetitive DNA was cloned from HindIII-digested genomic DNA of Larix leptolepis. The repetitive DNA was about 170 bp long, had an AT content of 67%, and was organized tandemly in the genome. Using fluorescence in situ hybridization and subsequent DAPI banding, the repetitive DNA was localized in DAPI bands at the proximal region of one arm of chromosomes in L. leptolepis and Larix chinensis. Southern blot hybridization to genomic DNA of seven species and five varieties probed with cloned repetitive DNA showed that the repetitive DNA family was present in a tandem organization in genomes of all Larix taxa examined. In addition to the 170-bp sequence, a 220-bp sequence belonging to the same DNA family was also present in 10 taxa. The 220-bp repeat unit was a partial duplication of the 170-bp repeat unit. The 220-bp repeat unit was more abundant in L. chinensis and Larix potaninii var. macrocarpa than in other taxa. The repetitive DNA composed 2.0–3.4% of the genome in most taxa and 0.3 and 0.5% of the genome in L. chinensis and L. potaninii var. macrocarpa, respectively. The unique distribution of the 220-bp repeat unit in Larix indicates the close relationship of these two species. In the family Pinaceae, the LPD (Larix proximal DAPI band specific repeat sequence family) family sequence is widely distributed, but their amount is very small except in the genus Larix. The abundant LPD family in Larix will occur after its speciation.Key words: AT-rich tandem repetitive DNA, fluorescence in situ hybridization, Larix, proximal DAPI band.

Human Papillomavirus (HPV) Cervical Lesions: Results from 300 Italian Women Studied with Dna Hybridization Techniques and Morphology

1988 ◽  
Vol 74 (6) ◽  
pp. 745-749 ◽  
Author(s):  
Fiorella Nuzzo ◽  
Vittorio Tison ◽  
Antonella Castagnoli ◽  
Maurizio Tiboni ◽  
Ethel-Michele De Villiers ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Human Papillomavirus ◽  
Southern Blot ◽  
Dna Hybridization ◽  
Blot Hybridization ◽  
Hpv Infection ◽  
Cervical Lesions ◽  
Hpv Dna ◽  
Cervical Infection

Human papillomavirus cervical infection was investigated in a series of 300 unselected women by comparing morphological diagnoses (cytology and histology) with results of DNA hybridization techniques (filter in situ hybridization of DNA from exfoliated cervical cells and Southern blot analysis of HPV-DNA in cervical biopsy specimens). The prevalence of HPV cervical infection diagnosed by PAP smears was 11.6 %. Despite disadvantages, filter in situ hybridization was confirmed to be particularly useful for screening purposes to detect HPV in cervical scrapings. In 3 cases it was the only applicable method for diagnosing « high-risk » HPV infection. Southern blot hybridization of tissue DNA with HPV 16-DNA revealed the presence of this virus in 8 cases, and HPV 31-DNA and HPV 42-DNA in 1 case each.

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