scholarly journals Adaptive Evolution and Addressing the Relevance for Genetic Improvement of Sago Palm Commodity

2020 ◽  
Author(s):  
Barahima Abbas
Keyword(s):  
Molecular Marker ◽  
Genetic Resources ◽  
Adaptive Evolution ◽  
Genetic Improvement ◽  
Marker Assisted Breeding ◽  
Sago Palm ◽  
Vernacular Names ◽  
Cpdna Markers ◽  
Metroxylon Sagu ◽  
Starch Production

Adaptive evolution implies evolutionary shifts within an organism which make it suitable and adaptable for its environment. Genetic resources of sago palm (Metroxylon sagu Rottb.) populations in Indonesia were explicated as follows: (1) Characters of sago palm in Indonesia were shown varied based on cpDNA markers and large variation based on RAPD markers. (2) Variation of starch production of sago palm correlated with Wx genes variation, (3) Distances barrier and geographies isolation in line of sago palm dispersions in Indonesia (4) Characteristics of genetic were observed does not related with vernacular names those were given by local people (5) Papua islands, Indonesia territorial is proposed the center of sago palm diversities, (6) Papua islands, Sulawesi islands and Kalimantan islands will be the provenance of the diversities (7) Genetic improvement of sago palm might enhanced using molecular marker that link to interesting genes by developing marker-assisted breeding.

scholarly journals Short Communication: A comparative study of phenotypes and starch production in sago palm (Metroxylon sagu) growing naturally in temporarily inundated and non inundated areas of South Sorong, Indonesia

2019 ◽  
Vol 20 (4) ◽  
pp. 1121-1126
Author(s):  
TOMAS YATER ◽  
HERMAN WAFOM TUBUR ◽  
CIPTA MELIALA ◽  
BARAHIMA ABBAS
Keyword(s):  
Comparative Study ◽  
Short Communication ◽  
Morphological Characters ◽  
Middle Part ◽  
Starch Accumulation ◽  
Sago Palm ◽  
Vernacular Names ◽  
West Papua ◽  
Metroxylon Sagu ◽  
Starch Production

Abstract. Yater T, Tubur HW, Meliala C, Abbas B. 2019. Short Communication:  A comparative study of phenotypes and starch production in sago palm (Metroxylon sagu) growing naturally in temporarily inundated and non-inundated areas of South Sorong, Indonesia. Biodiversitas 20:  1121-1126. Sago palm forests and sago palm semi cultivation are generally spread in swampy areas, seasonally inundated areas and non-inundated areas. The objectives of this study are to determine and compare the phenotypes of and starch production by sago palms growing naturally in the temporarily inundated areas (TIA type) and non-inundated areas (WIA type) in South Sorong District, West Papua Province, Indonesia. Sago palms of both habitats were found to be the same variety based on analysis of vernacular names and general characteristics. Comparison of morphological characters related to starch production of TIA and WIA types showed that there were no significant differences between the two studied types. The distribution of starch along the sago trunk was observed to be uneven, higher starch accumulation was found in the middle part of the trunk which was significantly different from the lower and upper parts of the trunk.

KONDISI LAHAN TUMBUHAN SAGU DI DESA RUMAHKAY KECAMATAN AMALATU KABUPATEN SERAM BAGIAN BARAT PROVINSI MALUKU

2017 ◽  
Vol 13 (2) ◽  
pp. 84-93
Author(s):  
Meilisa Nusawakan ◽  
Pieter Kunu ◽  
Marcus Luhukay
Keyword(s):  
Consumption Patterns ◽  
Survey Method ◽  
Sago Starch ◽  
Main Food ◽  
Sago Palm ◽  
Average Production ◽  
Metroxylon Sagu ◽  
Starch Production

This study aims to map the condition of land where sago plant grows, to determine the suitability level of sago plant land and to describe the potential of sago and sago consumption patterns by the community. The method used in this research is survey method with distance observation free survey and pit profile observation type. The condition of the land where sago plants grow is quite good. The types of sago found are sagu tuni (Metroxylon rumphii Mart.), Sagu Ihur (Metroxylon sylvestre Mart.) And sagu molat (Metroxylon sagu Rottb.). The size of sago palm in Rumahkay Village is 55.5 Ha, the average number of cutting trees (MT) 24 trees / Ha / yr with average production of wet starch per tree sebesr 700 kg. Total dry starch production at the study site was 449.55 tons. The pattern of community consumption of sago 10 percent, the combination of sago, tuber and banana by 20 percent, the combination of sago, tubers, bananas and rice by 55 percent and rice 10 percent. Types of confectionery and food based sago starch consumed is papeda, sago plate, sinoli and karu-karu. Frequency and time to eat sago as main food and food complement of 65% is as much as 2 times in a day that is time of morning and afternoon. Then 3 times as much as 25% and once as much as 10%. In general, people who consume sago once a day is at breakfast or afternoon in the form of snacks (sago plate, sinoli, karu-karu). Keywords: condition, land, sago   ABSTRAK Penelitian ini bertujuan untuk memetakan kondisi lahan tempat tumbuh tumbuhan sagu, menetapkan tingkat kesesuaian lahan tumbuhan sagu serta mendeskripsikan potensi sagu dan pola konsumsi sagu oleh masyarakat. Metode yang digunakan dalam penelitian ini adalah metode survei dengan jarak observasi survei bebas dan tipe observasi profil pit. Kondisi lahan tempat tumbuh tumbuhan sagu tergolong baik. Jenis sagu yang ditemukan adalah sagu Tuni (Metroxylon rumphii Mart.), sagu Ihur (Metroxylon sylvestre Mart.) dan sagu Molat (Metroxylon sagu Rottb.). Luas lahan sagu di Desa Rumahkay adalah 55,5 Ha, rata-rata jumlah pohon masak tebang (MT) 24 pohon/Ha/thn dengan rata-rata produksi pati basah per pohon sebesr 700 kg. Total produksi pati kering pada lokasi penelitian adalah 449,55 ton. Pola konsumsi masyarakat terhadap sagu 10 persen, kombinasi sagu, umbian dan pisang sebesar 20 persen, kombinasi sagu, umbian, pisang dan beras sebesar 55 persen serta beras 10 persen. Jenis penganan dan pangan berbahan dasar pati sagu yang dikonsumsi adalah papeda, sagu lempeng, sinoli dan karu-karu. Frekwensi dan waktu makan sagu sebagai pangan utama maupun pangan pelengkap sebesar 65% adalah sebanyak 2 kali dalam sehari yaitu waktu pagi dan siang. Kemudian 3 kali sebanyak 25% dan satu kali sebanyak 10%. Pada umumnya mereka yang mengkonsumsi sagu satu kali dalam sehari adalah pada saat sarapan pagi atau sore hari dalam bentuk penganan (sagu lempeng, sinoli, karu-karu). Kata kunci: kondisi, lahan, sagu

scholarly journals Ekspresi dan kloning gen penyandi ADP-Glucose Phyrophosphorylase dari tanaman sagu (Metroxylon sagu Rottb.) Expression and cloning of gene encoding ADP-Glucose Phyrophosphorylase from sago palm (Metroxylon sagu Rottb.)

2016 ◽  
Vol 83 (2) ◽  
Author(s):  
Asmini BUDIANI1 ◽  
Riza Arief PUTRANTO ◽  
Hayati MINARSIH ◽  
Imron RIYADI ◽  
. SUMARYONO ◽  
...  
Keyword(s):  
Molecular Marker ◽  
Dna Sequence ◽  
Starch Content ◽  
Gene Encoding ◽  
Sago Palm ◽  
High Starch Content ◽  
Long Time ◽  
Time Availability ◽  
High Starch ◽  
Metroxylon Sagu

AbstractSago palm (Metroxylon sagu Rottb.) is a potential food and energy resources becouse it is the highest starch producing plant.  Breeding of sago palm should be directed to produce elite genotype with superior characters such as high starch content, wider pith diameter, without spine and high starch quality. However, research on sago palm in Indonesia so far is limited espescially in the field of cultivation and breeding, and attempt to produce such elite would take long time. Availability of molecular marker for starch content would be beneficial to shorten the length period of breeding. ADP-Glucose Phyrophosphorylase is one of the important enzymes in starch biosynthesis. Therefore its gene is an interesting subject in order to develope molecular marker of high starch content.  This research was aimed to study the expression of gene encoding AGP in the sago palm with high starch content versus low starch content, and to clone the full cds of the gene. RNA was isolated from leaf and pith of both palms. Exspression analysis and amplify-cation of full cds were conducted by Reverse Transcryptase-Polymerase Chain Reaction (RT-PCR) using specific primers. The results showed that sago palm with higher starch content expressed AGP higher than that of sago palm with lower  starch content. Expression of AGP in the full developing leaf was higher than in the young leaf, and there was no expression detected in the pith. The full cds of AGP was successfully amplified and cloned. Even though the DNA sequence showed high homology with DNA sequence of the same gene that has been deposited in GenBank, there were differences in severall nucleotide including that in the active domain of the enzyme.AbstrakTanaman sagu merupakan sumber pangan dan energi yang sangat potensial untuk dikembangkan karena merupakan tanaman penghasil karbihidrat tertinggi. Pemuliaan tanaman sagu mestinya diarah-kan untuk menghasilkan bibit sagu yang selain memiliki rendemen pati tinggi, juga memiliki diameter empulur besar, tidak berduri dan memiliki cita rasa pati yang enak. Namun, sampai saat ini riset mengenai sagu di Indonesia masih sangat terbatas, sehingga pemuliaan sagu untuk menghasilkan bibit unggul demikian akan memerlukan waktu lama. Ketersediaan penanda rendemen pati akan sangat membantu mempercepat pemuliaan tanaman tersebut. ADP-Glucose Pyrophosphorylase adalah salah satu enzim yang berperan penting dalam biosintesis pati, sehingga gene penyandinya merupakan subjek yang menarik dalam pengembangan marka kandungan pati tinggi.  Sebagai bagian dari upaya untuk mendapat-kan penanda rendemen pati tinggi pada tanaman sagu, penelitian ini bertujuan untuk mempelajari ekspresi gen penyandi AGP. RNA diisolasi dari daun tanaman sagu rendemen pati rendah dan tanaman sagu rendemen pati tinggi. Perbedaan tingkat ekspresi gen penyandi AGP dari tanaman sagu rendemen pati tinggi vs rendemen pati rendah, dianalisis dengan teknik Reverse-Transcryptase PCR menggunakan primer spesifik. Hasil penelitian menunjukkan bahwa tanaman sagu rendemen pati tinggi mengekspresikan AGP lebih tinggi dibandingkan dengan tanaman sagu rendemen pati rendah. Ekspresi gen tersebut pada daun tua (full developing leaf) lebih tinggi di-bandingkan dengan pada daun muda, dan pada empulur tidak dideteksi ekspresi gen tersebut. Daerah penyandi lengkap AGP subunit kecil telah diklon. Meskipun memiliki homologi yang tinggi dengan sekuen DNA gen yang sama yang telah dideposit pada  GenBank,  namun terdapat perbedaan beberapa nukleotida termasuk pada daerah domain aktif dari enzim tersebut. 

scholarly journals Ekspresi dan kloning gen penyandi ADP-Glucose Phyrophosphorylase dari tanaman sagu (Metroxylon sagu Rottb.) Expression and cloning of gene encoding ADP-Glucose Phyrophosphorylase from sago palm (Metroxylon sagu Rottb.)

2016 ◽  
Vol 83 (2) ◽  
Author(s):  
Asmini BUDIANI1 ◽  
Riza Arief PUTRANTO ◽  
Hayati MINARSIH ◽  
Imron RIYADI ◽  
. SUMARYONO ◽  
...  
Keyword(s):  
Molecular Marker ◽  
Dna Sequence ◽  
Starch Content ◽  
Gene Encoding ◽  
Sago Palm ◽  
High Starch Content ◽  
Long Time ◽  
Time Availability ◽  
High Starch ◽  
Metroxylon Sagu

AbstractSago palm (Metroxylon sagu Rottb.) is a potential food and energy resources becouse it is the highest starch producing plant.  Breeding of sago palm should be directed to produce elite genotype with superior characters such as high starch content, wider pith diameter, without spine and high starch quality. However, research on sago palm in Indonesia so far is limited espescially in the field of cultivation and breeding, and attempt to produce such elite would take long time. Availability of molecular marker for starch content would be beneficial to shorten the length period of breeding. ADP-Glucose Phyrophosphorylase is one of the important enzymes in starch biosynthesis. Therefore its gene is an interesting subject in order to develope molecular marker of high starch content.  This research was aimed to study the expression of gene encoding AGP in the sago palm with high starch content versus low starch content, and to clone the full cds of the gene. RNA was isolated from leaf and pith of both palms. Exspression analysis and amplify-cation of full cds were conducted by Reverse Transcryptase-Polymerase Chain Reaction (RT-PCR) using specific primers. The results showed that sago palm with higher starch content expressed AGP higher than that of sago palm with lower  starch content. Expression of AGP in the full developing leaf was higher than in the young leaf, and there was no expression detected in the pith. The full cds of AGP was successfully amplified and cloned. Even though the DNA sequence showed high homology with DNA sequence of the same gene that has been deposited in GenBank, there were differences in severall nucleotide including that in the active domain of the enzyme.AbstrakTanaman sagu merupakan sumber pangan dan energi yang sangat potensial untuk dikembangkan karena merupakan tanaman penghasil karbihidrat tertinggi. Pemuliaan tanaman sagu mestinya diarah-kan untuk menghasilkan bibit sagu yang selain memiliki rendemen pati tinggi, juga memiliki diameter empulur besar, tidak berduri dan memiliki cita rasa pati yang enak. Namun, sampai saat ini riset mengenai sagu di Indonesia masih sangat terbatas, sehingga pemuliaan sagu untuk menghasilkan bibit unggul demikian akan memerlukan waktu lama. Ketersediaan penanda rendemen pati akan sangat membantu mempercepat pemuliaan tanaman tersebut. ADP-Glucose Pyrophosphorylase adalah salah satu enzim yang berperan penting dalam biosintesis pati, sehingga gene penyandinya merupakan subjek yang menarik dalam pengembangan marka kandungan pati tinggi.  Sebagai bagian dari upaya untuk mendapat-kan penanda rendemen pati tinggi pada tanaman sagu, penelitian ini bertujuan untuk mempelajari ekspresi gen penyandi AGP. RNA diisolasi dari daun tanaman sagu rendemen pati rendah dan tanaman sagu rendemen pati tinggi. Perbedaan tingkat ekspresi gen penyandi AGP dari tanaman sagu rendemen pati tinggi vs rendemen pati rendah, dianalisis dengan teknik Reverse-Transcryptase PCR menggunakan primer spesifik. Hasil penelitian menunjukkan bahwa tanaman sagu rendemen pati tinggi mengekspresikan AGP lebih tinggi dibandingkan dengan tanaman sagu rendemen pati rendah. Ekspresi gen tersebut pada daun tua (full developing leaf) lebih tinggi di-bandingkan dengan pada daun muda, dan pada empulur tidak dideteksi ekspresi gen tersebut. Daerah penyandi lengkap AGP subunit kecil telah diklon. Meskipun memiliki homologi yang tinggi dengan sekuen DNA gen yang sama yang telah dideposit pada  GenBank,  namun terdapat perbedaan beberapa nukleotida termasuk pada daerah domain aktif dari enzim tersebut. 

scholarly journals Photosynthesis of Sago Palm (Metroxylon sagu Rottb.) Seedling at Different Air Temperatures

Agriculture ◽  
2018 ◽  
Vol 8 (1) ◽  
pp. 4 ◽  
Author(s):  
Aidil Azhar ◽  
Daigo Makihara ◽  
Hitoshi Naito ◽  
Hiroshi Ehara
Keyword(s):  
Sago Palm ◽  
Air Temperatures ◽  
Metroxylon Sagu

scholarly journals Differential Metabolites Markers from Trunking and Stressed NonTrunking Sago Palm (Metroxylon sagu Rottb.)

2020 ◽  
Vol 14 ◽  
Author(s):  
Hasnain Hussain ◽  
Wei-Jie Yan ◽  
Zainab Ngaini ◽  
Norzainizul Julaihi ◽  
Rina Tommy ◽  
...  
Keyword(s):  
Extraction Method ◽  
Methanol Extract ◽  
Economic Value ◽  
Principal Component ◽  
Leaf Extracts ◽  
Sulfurous Acid ◽  
Sago Palm ◽  
Hexyl Ester ◽  
Metroxylon Sagu ◽  
Metabolite Markers

Background: Sago palm is an important agricultural starch-producing crop in Malaysia. The trunk of sago palm is responsible for the production of the starch reaching maturity for harvesting after ten years. However, there are sago palms that failed to develop its trunk after 17 years being planted. This is known as a stressed “non-trunking” sago palm, which eliminates the economic value of the palms. Objective: The study was initiated to compare the differences in metabolite expression between trunking and non-trunking sago palm and secondly to determine the potential metabolite-makers that are related to differential phenotypes of sago palms. Method: Metabolites were extracted using various solvents and analysed using NMR spectroscopy and GC-MS spectrometry. Data obtained were subjected to principal component analysis. Results: The study determined that differential metabolites expression were detected in the leaf extracts of normal trunking sago palm compared to the non-trunking palms. Metabolite groups which are differently expressed between trunking and non-trunking sago palm are oils and waxes, haloalkanes, sulfite esters, phosphonates, phosphoric acid, thiophene ester, terpenes and tocopherols. GC-MS analysis of Jones & Kinghorn extraction method determined two sets of metabolite markers which explains the differences in metabolites expression of trunking and non-trunking sago palm in ethyl acetate and methanol extract of 89.55% comprising sulfurous ester compounds and 87.04% comprising sulfurous ester, sulfurous acid and cyclohexylmethyl hexyl ester respectively. Conclusion: Two sets of metabolite markers were expressed in the trunking and non-trunking sago palm. These metabolites can potentially be used as markers for identifying normal and stressed plants.

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