Characteristics of avocado (Persea americana) and kluwih (Artocarpus camansi) seeds starch with different extraction methods

Seed from avocado (Persea Americana) and kluwih (Artocarpus camansi) fruit are waste and by product from processing fruit. Due to the high starch content from both seeds, there are many extractions process have been applied. The immersed raw material in sodium metabisulfite solution affected to starch properties. The purpose of this study is to characterize of starch extracted with and without immersed in sodium metabisulfite solution. The results showed that the starch yield by immersed in alkaline solution was 21.6% for avocado seed starch and 24.4% for kluwih seed starch, while yield without immersed in sodium metabisulfite solution was 17.5% and 18,2% for avocado seed starch and kluwih seed starch, respectively. The starch content with sodium metabisulfite immersion process of avocado seed and kuwih seed were 79.26% and 72.62%, respectively. Meanwhile without the immersion process resulted starch of 75.13 and 67.30%, in avocado seed starch and kluwih seed starch, respectively. Furthermore, XRD and FTIR analysis were also carried out on starch to see the crystallinity character and functional groups contained in the extracted starch.

Identification of starch candidate genes using SLAF-seq and BSA strategies and development of related SNP-CAPS markers in tetraploid potato

Potato starch is an essential nutrient for humans and is widely used worldwide. Locating relevant genomic regions, mining stable genes and developing candidate gene markers can promote the breeding of new high-starch potato varieties. A total of 106 F1 individuals and their parents (YSP-4 × MIN-021) were used as test materials, from which 20 plants with high starch content and 20 with low starch content were selected to construct DNA pools for site-specific amplified fragment sequencing (SLAF-seq) and bulked segregation analysis (BSA). A genomic region related to the starch traits was first identified in the 0–5.62 Mb of chromosome 2 in tetraploid potato. In this section, a total of 41 non-synonymous genes, which were considered as candidate genes related to the starch trait, were annotated through a basic local alignment search tool (BLAST) search of multiple databases. Six candidate genes for starch (PGSC0003DMG400017793, PGSC0003DMG400035245, PGSC0003DMG400036713, PGSC0003DMG400040452, PGSC0003DMG400006636 and PGSC0003DMG400044547) were further explored. In addition, cleaved amplified polymorphic sequence (CAPS) markers were developed based on single nucleotide polymorphism (SNP) sites associated with the starch candidate genes. SNP-CAPS markers chr2-CAPS6 and chr2-CAPS21 were successfully developed and validated with the F2 population and 24 tetraploid potato varieties (lines). Functional analysis and cloning of the candidate genes associated with potato starch will be performed in further research, and the SNP-CAPS markers chr2-CAPS6 and chr2-CAPS21 can be further used in marker-assisted selection breeding of tetraploid potato varieties with high starch content.

Uji Kinerja Mesin Pengiris dan Pemarut Ganyong (MPPG) Tep-02

Canna (Canna edulis Kerr.) is a type of tuber plant with a fairly high starch content to be used as food. The use of canna is generally processed first into flour. The initial process of flouring canna is done in two ways; the canna plants are sliced and grated. The Department of Agricultural and Biosystem Engineering, Faculty of Agricultural Industrial Technology, University of Padjadjaran, designed the TEP-1902 Canna Slicing and Shredding Machine (MPPG). However, the performance of this machine was not yet known, so testing was needed to determine its performance. The research method used was an experimental method that was analyzed descriptively by measuring, observing, calculating, analyzing, testing, and evaluating the TEP-1902 Canna Slicing and Shredding Machine (MPPG). The results of the performance test of this machine showed that the machine's performance was good, with the following parameters: the actual capacity of the slicing unit and grating unit was 40.95 kg/hour and 182.98 kg/hour, respectively, efficiency was 92.61% slicing and 97.60% grater, the yields were 98.75% slicing and 96.50% grating, respectively, the average thickness of the slices was 2.13 mm, and the uniformity of slice thickness was 65.51%.

Continuous Blown Film Preparation of High Starch Content Composite Films with High Ultraviolet Aging Resistance and Excellent Mechanical Properties

Starch/PBAT blown films with high ultraviolet aging resistance and excellent mechanical properties were prepared by introducing lignin with polyurethane prepolymer (PUP) as a starch modifier and physical compatibilizer and 4,4′–methylene diphenyl diisocyanate (MDI) as a crosslinker. Starch was modified by reacting the NCO groups of the PUP with the OH groups of the starch to form a carbamate bond. The mechanical properties, hydrophobic properties, ultraviolet barrier, ultraviolet aging properties and microscopic morphology of starch/PBAT films with different contents of lignin were investigated. The results showed that the starch/PBAT films were blown continuously. The addition of lignin did not decrease the mechanical properties. On the contrary, the film with 1% lignin possessed the excellent mechanical properties with longitudinal tensile strength of 15.87 MPa and the elongation at a break of 602.21%. In addition, the higher the lignin content, the better the UV blocking effect. The introduction of lignin did not affect the crystalline properties but improved the hydrophilic properties and sealing strength of the high starch content composite films.

A Life Cycle Assessment (LCA) Approach on the Production of Sago Sucker for Cultivation

Sago palm is one of the commodities being cultivated and traded in Malaysia for its high starch content. Sago seedling, or commonly referred to as sucker, is the planting material for sago cultivation. This paper discusses the life cycle assessment approach for the calculation of life cycle inventory for the production of one sago sucker. In this study, the functional unit is defined as one sago sucker. The process starts from the reception of suckers from suppliers, raft preparation, planting sago suckers in rafts, fertilization, and ends with transportation of the suckers to the plantation. Interviews and data verification were done on-site at the Crop Research and Application Unit (CRAUN) Sungai Talau Research Station. LCI data showed that water was the main input for the production of sago sucker, followed by diesel for transportation, pesticides, and fertilisers. The outcome of this study provides a basis or guideline to planters in implementing sago best agricultural practices for the production of sustainable sago.

STUDI OPTIMALISASI KOMPOSISI GLISEROL DAN KITOSAN TERHADAP KARAKTERISTIK SIFAT FISIK PLASTIK BIODEGRADABLE DARI LIMBAH NASI AKING DAN TEPUNG TAPIOKA

Aking rice is rice waste that is no longer suitable for public consumption, however, the high starch content of aking rice can be used as a raw material for making biodegradable plastics. Good biodegradable plastic made from cassava starch and rice flour, to increase its elasticity and extensibility it is necessary to add chitosan additives and glycerol plasticizers. This study aims to determine the optimum conditions for the composition of glycerol and chitosan for the physical properties of biodegradable plastics made from aking rice and tapioca starch. Biodegradable plastics in this study were made with 3 grams of aking rice flour and 7 grams of tapioka flour with the addition of variations in the composition of chitosan (3 grams, 5 grams, and 7 grams) and the addition of variations in the composition of glycerol (2 ml, 4 ml, 6 ml, and 8 ml). As a result, the highest tensile strength value at 7: 2 composition variation is 41.29 MPa, the highest elongation percentage at 3: 8 composition variation is 135.8%, the highest water resistance at 7: 8 composition variation is -206%, the highest percent water absorption and the fastest biodegradation process at a variation of the composition of 3: 2, namely 1603% and completely degraded on the 4th day.Keywords: aking rice, biodegradable plastic, chitosan, glycerol and tapioca starch.

The estimation of the new self-pollinated waxy maize lines and hybrids (Zea mays l. Ceratina)

Though waxy maize is a valuable raw material for the starch industry, its subspecies are still poorly studied. The purpose of the current work was to study the new self-pollinated hybrids and lines of waxy maize according to a complex of economically valuable traits and to identify promising ones for further breeding. The study was carried out at the FSBSI “Agricultural Research Center “Donskoy” in 2019–2020. The objects of research were 40 self-pollinated waxy maize lines and 30 hybrids developed in the Center. They were studied according to a set of economically valuable traits. There have been identified the self-pollinated lines (24/82, 26/18, 26/51, 24/15) with a high kernel productivity of 0.91–2.23 t/ha, low and medium harvest moisture content of kernel (14.0–14.7%), high resistance to lodging (0–4.6% of lodged plants), weak infestation with smut on a natural background (0–5.0% of damaged plants). There have been identified the lines (24/15, 26/18), characterized by a high starch content in matured kernel (69.2% and 69.8%). There have been developed the new waxy maize hybrids (91×93, 103×105), which significantly exceeded the standard hybrid ‘Zernogradsky 282 MB’ both in kernel productivity (on 0.41–0.80 t/ha) and in the yield of amylopectin starch (on 0, 20–0.54 t/ha). The new hybrids were characterized with low harvest moisture content of kernels (13.3–13.9%), resistance to lodging and smut on a natural background. There have been identified the new maize hybrids (91 × 94, 91×93, 91×95) with a high starch content (68.5–68.9%), an average oil content (4.4–4.6%) and protein percentage (12.1–12.2%) in matured kernel.

The results and prospects of maize breeding in the Agricultural Research Center “Donskoy”

The current paper has presented the results of breeding work on maize in the Agricultural Research Center “Donskoy”. The three-line and double interlinear middle early and middle ripening maize hybrids of various economic use ‘Zernogradsky 242 MV’, ‘Zernogradsky 282 MV’, ‘Zernogradsky 288 MV’, ‘Zernogradsky 299 MV’, ‘Zernogradsky 354 MV’, ‘Zernogradsky MV’, ‘Stepnyak MV’ were developed and included into the State List of Breeding Achievements from 2006 to 2020. The Competitive Variety Testing has identified a new high-heterosis simple hybrid ‘Zernogradsky 352 MB’ and the new early ripening hybrids ‘Krucha M × KB 215’, ‘Krucha M × SP 56/57’ with low harvesting grain moisture. The main method used to develop maize hybrids was an interline hybridization using heterosis in the first generation (F1 ). There have been determined the priority directions of further breeding, namely early ripeness, grain humidity-transfer intensity during ripening, drought resistance, high starch content in grain. There has been developed new initial material for each direction of breeding. There have been selected the introduced testers (‘Alpha M’, ‘Almaz M’, ‘Aurora S’, ‘Milena M’, ‘Istok S’) to develop early-ripening hybrids. There have been identified the early and middle-early ripening self-pollinated lines ‘C 207’, ‘RD 12’, ‘TVA 308’, ‘PLS 61’, ‘KB 215’, etc., with a high grain humidity-transfer intensity during ripening (0.95–1.30% per day), low harvest moisture content of grain (10.8–13.8%). There have been developed the new self-pollinated waxy maize lines (24/29/5, 25/64/10, 26/8, 26/4, 26/80, 24/28) with 68.1–69.8% of amylopectin starch in grain. There have been identified the new self-pollinated maize lines resistant to water stress (DS 498/217-3, DS 257/85-5, DS 498/203, KB 262, etc.) promising for breeding drought-resistant maize hybrids.

The study of the source material of potatoes under conditions of Kirov region

The article presents the results of study of potato varieties and hybrids collection in the conditions of Kirov region, including varieties of modern Russian and foreign collections, interspecific hybrids of VIR, Falenskaya selection station and other scientific institutions for 300-350 samples annually. The study was conducted in contrasting weather conditions in 2017-2019. Sources of high productivity (705…887 g/bush): 129-10, Effekt (Russia), Lukawa (Czechoslovakia), Zorachka, Uladar (Belarus), Colomba (Netherlands) and others, and large potato tuber (100…140 g): Bellarosa (Germany), Lilea (Belarus), Irbitsky, Krepysh (Russia) were revealed. Due to big epiphytotic of late blight in 2017 varieties with high field resistance of foliage to late blight were revealed (7-9 points): Kufri Anara (India), Suzorye, Ragneda, Alpinist (Belarus), Gubernator, Skazka, Effekt, Vdohnovenie, Fritella, Legenda, 90-5-30, 55-03 (Russia); 387110.4, 678019 (Peru) and others. In 2019 samples with high late blight resistance of tubers were selected (9 points): Lilea (Belarus), Udacha, Ognivo, Lubava Legenda, 122-29, 90-6-2, 194-00, 591-97, 55-03, 56-09, 172-11, 6-1, 39-10, 132-07 (Russia), 389746.2, 678019 (BL-22), 678009 (BL-1.10) (Peru). Varieties of field resistance to viral diseases were selected: Lukawa (Czechoslovakia), Yanka, Ragneda, Zorachka, Charaunik (Belarus), Franzi, Albina, Kolomba, Sofia (Germany), Lazar, Skazka, Mayak, Udacha, Krasavchik, Charodey), Zolsky, Matushka, Nayada, Sudarynya, Kolobok, 42-7-40, 112-04, 184-05, 28-06, 48-224-10, 455-08, 431-08, 142-09, 5-7К, 1-13К, 218-12, 286-08, 40-14К, 16-25-1К, 16-29-33К, 56-09, 289-13. Assessment of starch content allowed to select varieties and hybrids with starch content above 20%: Bashkirsky, Zolsky, Lazar (Russia), Uladar, Vektar Belorussky, Manifest, Zhuravinka, Zdabytok (Belarus) and others. Hybrids 268-09 and 289-13 combined high starch content and high late blight resistance. The most valuable source material for potato breeding in the Kirov region are varieties and hybrids with a complex manifestation of warnings: Bellarosa, Albina, Sofia (Germany), 129-09, 40-14, 7-2В, 5-7 К, 268-08, 141-14, 289-13, 455-08, 56-09, 149-14, Kamensky, Irbitsky, Debryansk, Effekt (Russia), Kolamba (Netherlands), Zorachka, Сharaunik, Uladar, Altair, Lilea (Belarus), Lukawa (Czechoslovakia).