scholarly journals Assessing the Vascular Deformability of Erythrocytes and Leukocytes: From Micropipettes to Microfluidics

2020 ◽  
Author(s):  
Mark D. Scott ◽  
Kerryn Matthews ◽  
Hongshen Ma
Keyword(s):  
Deformability Of Erythrocytes

Deformability of Erythrocytes is maintained after Autologous Cell Salvage in Orthopedic Surgery

2020 ◽  
Author(s):  
Sibylle Kietaibl
Keyword(s):  
Shear Stress ◽  
Cell Analysis ◽  
Cell Salvage ◽  
Hematological Disorders ◽  
Autologous Cell ◽  
Deformability Of Erythrocytes ◽  
Perioperative Bleeding ◽  
Elongation Index ◽  
Decrease Blood Loss ◽  
Human Red Cells

Background and Objective: Prerequisite for oxygen transport to the tissue is the ability of erythrocytes to change from a discoid into an elongated form in order to pass microvessels. Cell salvage is highly recommended to decrease blood loss and to avoid allogeneic transfusions in perioperative bleeding. The purpose of our study was to assess deformability of erythrocytes at re-transfusion. Materials and Methods: After ethics committee approval and informed consent, blood was withdrawn from the autotransfusion system (Xtra, Sorin, Germany) of 24 patients undergoing joint arthroplasty 6 hours after cell salvage initiation. Deformability curves, elongation indices (EI) obtained at increasing shear stress (SS) were assessed by using Laser Optical Rotational Red Cell Analysis (LORRCA). Results: Erythrocytes showed the typical sigmoid EI/SS curve with a mean maximum elongation index (EImax) of 0.593 ± 0.034 and mean half-maximal deformation (SS ½) of 1.186 ± 0.387 Pa. Irregular curve shapes and high variability in EI occurred at shear stress < 3 Pa. Recalculation of outcome parameters resulted in higher values (EImax 0.610 ± 0.035; SS ½ 1.562 ± 0.346 Pa). Conclusion: Erythrocytes after autologous cell salvage are not stiff but elongate in response to shear stress. Erythrocytes showed the typical EI/SS-curve of human red cells, which indicates that the cells are able to uptake the hydrodynamic forces that act on them during the measurement. Further research is needed to investigate the effect of cell salvage processing on erythrocytes from patients with pre-existing hematological disorders.

Impaired deformability of erythrocytes in diabetic rat and human: investigation by the nickel-mesh-filtration technique

2011 ◽  
Vol 25 (1-2) ◽  
pp. 18-26 ◽  
Author(s):  
K. Saito ◽  
Y. Kogawa ◽  
M. Fukata ◽  
K. Odashiro ◽  
Toru Maruyama ◽  
...  
Keyword(s):  
Diabetic Rat ◽  
Deformability Of Erythrocytes ◽  
Filtration Technique

Osmotic deformability of erythrocytes at various shear stresses

2015 ◽  
Vol 59 (3) ◽  
pp. 211-218 ◽  
Author(s):  
Yujin Heo ◽  
Hyunwoo Jung ◽  
Sehyun Shin
Keyword(s):  
Shear Stresses ◽  
Deformability Of Erythrocytes

Membrane Deformability of Erythrocytes with Glucose-6-Phosphate Dehydrogenase Hamburg

1977 ◽  
Vol 57 (3) ◽  
pp. 162-167 ◽  
Author(s):  
W. Tillmann ◽  
M. Gahr ◽  
N. Labitzke ◽  
W. Schröter
Keyword(s):  
Deformability Of Erythrocytes ◽  
Glucose 6 Phosphate Dehydrogenase

scholarly journals PMCA activity and membrane tubulin affect deformability of erythrocytes from normal and hypertensive human subjects

2015 ◽  
Vol 1848 (11) ◽  
pp. 2813-2820 ◽  
Author(s):  
Noelia E. Monesterolo ◽  
Ayelen D. Nigra ◽  
Alexis N. Campetelli ◽  
Verónica S. Santander ◽  
Juan F. Rivelli ◽  
...  
Keyword(s):  
Human Subjects ◽  
Deformability Of Erythrocytes ◽  
Membrane Tubulin

Altered Behaviour of Erythrocytes in Scleroderma

1983 ◽  
Vol 65 (5) ◽  
pp. 515-519 ◽  
Author(s):  
I. B. Kovacs ◽  
S. O. Sowemimo-Coker ◽  
J. D. T. Kirby ◽  
P. Turner
Keyword(s):  
Endothelial Cells ◽  
Systemic Sclerosis ◽  
Sialic Acid ◽  
Raynaud’S Phenomenon ◽  
Progressive Systemic Sclerosis ◽  
Normal Subjects ◽  
Vascular Abnormalities ◽  
Cultured Endothelial Cells ◽  
Deformability Of Erythrocytes ◽  
Electrophoretic Velocity

1. Filterability (deformability) of erythrocytes of patients with Raynaud's phenomenon together with progressive systemic sclerosis (PSS) was decreased compared with the filtration of erythrocytes from normal subjects. 2. PSS erythrocytes showed lower electrophoretic velocity and about 23% less neuraminidase-removable sialic acid density on their surface than the normal erythrocytes. 3. PSS erythrocytes showed more adherence to cultured endothelial cells than the control normal erythrocytes. 4. It is concluded that the increased rigidity and adherence of PSS erythrocytes-5-have pathological significance in the mechanism of vascular abnormalities in PSS.

A possible correlation of electro-optic changes with the deformability of erythrocytes

1990 ◽  
Vol 16 (3) ◽  
pp. 161-167 ◽  
Author(s):  
I. Dzhene ◽  
R. Petrova ◽  
S. Stoylov
Keyword(s):  
Deformability Of Erythrocytes ◽  
Electro Optic
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