Reduction of deformability of erythrocytes of ischemic rats under the action of semax: examination by the method of laser diffractometry

2006 ◽  
Author(s):  
A. E. Lugovtsov ◽  
A. V. Priezzhev ◽  
O. E. Fadukova ◽  
V. B. Koshelev
Keyword(s):  
Laser Diffractometry ◽  
Deformability Of Erythrocytes

Deformability of Erythrocytes is maintained after Autologous Cell Salvage in Orthopedic Surgery

2020 ◽  
Author(s):  
Sibylle Kietaibl
Keyword(s):  
Shear Stress ◽  
Cell Analysis ◽  
Cell Salvage ◽  
Hematological Disorders ◽  
Autologous Cell ◽  
Deformability Of Erythrocytes ◽  
Perioperative Bleeding ◽  
Elongation Index ◽  
Decrease Blood Loss ◽  
Human Red Cells

Background and Objective: Prerequisite for oxygen transport to the tissue is the ability of erythrocytes to change from a discoid into an elongated form in order to pass microvessels. Cell salvage is highly recommended to decrease blood loss and to avoid allogeneic transfusions in perioperative bleeding. The purpose of our study was to assess deformability of erythrocytes at re-transfusion. Materials and Methods: After ethics committee approval and informed consent, blood was withdrawn from the autotransfusion system (Xtra, Sorin, Germany) of 24 patients undergoing joint arthroplasty 6 hours after cell salvage initiation. Deformability curves, elongation indices (EI) obtained at increasing shear stress (SS) were assessed by using Laser Optical Rotational Red Cell Analysis (LORRCA). Results: Erythrocytes showed the typical sigmoid EI/SS curve with a mean maximum elongation index (EImax) of 0.593 ± 0.034 and mean half-maximal deformation (SS ½) of 1.186 ± 0.387 Pa. Irregular curve shapes and high variability in EI occurred at shear stress < 3 Pa. Recalculation of outcome parameters resulted in higher values (EImax 0.610 ± 0.035; SS ½ 1.562 ± 0.346 Pa). Conclusion: Erythrocytes after autologous cell salvage are not stiff but elongate in response to shear stress. Erythrocytes showed the typical EI/SS-curve of human red cells, which indicates that the cells are able to uptake the hydrodynamic forces that act on them during the measurement. Further research is needed to investigate the effect of cell salvage processing on erythrocytes from patients with pre-existing hematological disorders.

Impaired deformability of erythrocytes in diabetic rat and human: investigation by the nickel-mesh-filtration technique

2011 ◽  
Vol 25 (1-2) ◽  
pp. 18-26 ◽  
Author(s):  
K. Saito ◽  
Y. Kogawa ◽  
M. Fukata ◽  
K. Odashiro ◽  
Toru Maruyama ◽  
...  
Keyword(s):  
Diabetic Rat ◽  
Deformability Of Erythrocytes ◽  
Filtration Technique

Role of Organic Matter and Carbonates in Soil Aggregation Estimated Using Laser Diffractometry

Pedosphere ◽  
2011 ◽  
Vol 21 (5) ◽  
pp. 566-572 ◽  
Author(s):  
I. VIRTO ◽  
N. GARTZIA-BENGOETXEA ◽  
O. FERNÁNDEZ-UGALDE
Keyword(s):  
Organic Matter ◽  
Soil Aggregation ◽  
Laser Diffractometry

Laser diffractometry of erythrocyte deformation under hypo-osmotic hemolysis

2000 ◽  
Author(s):  
Stanislav S. Bessmeltsev ◽  
Alexander V. Lendiaev ◽  
Yulia A. Skvortsova ◽  
Vladimir A. Tarlykov
Keyword(s):  
Osmotic Hemolysis ◽  
Laser Diffractometry ◽  
Erythrocyte Deformation

Osmotic deformability of erythrocytes at various shear stresses

2015 ◽  
Vol 59 (3) ◽  
pp. 211-218 ◽  
Author(s):  
Yujin Heo ◽  
Hyunwoo Jung ◽  
Sehyun Shin
Keyword(s):  
Shear Stresses ◽  
Deformability Of Erythrocytes

scholarly journals Reduced deformability of thalassemic erythrocytes and erythrocytes with abnormal hemoglobins and relation with susceptibility to Plasmodium falciparum invasion

Blood ◽  
1992 ◽  
Vol 79 (9) ◽  
pp. 2460-2463 ◽  
Author(s):  
A Bunyaratvej ◽  
P Butthep ◽  
N Sae-Ung ◽  
S Fucharoen ◽  
Y Yuthavong
Keyword(s):  
Plasmodium Falciparum ◽  
Clinical Symptoms ◽  
Malarial Parasite ◽  
Beta Thalassemia ◽  
Cell Deformability ◽  
Intact Cells ◽  
Laser Diffractometry ◽  
Parasite Plasmodium ◽  
Hb E ◽  
Parasite Plasmodium Falciparum

Abstract A number of genetically variant erythrocytes showed decreased deformability of both intact cells and membranes prepared therefrom as measured by laser diffractometry. Erythrocytes associated with minor or no clinical symptoms (eg, alpha-thalassemia traits, hemoglobin [Hb] E trait, Hb Constant Spring trait), which showed only a minimal decrease in deformability, were, in general, invaded efficiently by the malarial parasite Plasmodium falciparum. Other variant erythrocytes (beta- thalassemia/Hb E, homozygous Hb E, homozygous Hb Constant Spring, Hb H, Hb H/Hb Constant Spring) with low deformability showed different degrees of reduction in invasion susceptibility, most of which were less than proportional with deformability decrease. It is concluded that parasite invasion is only weakly related to gross cell deformability, which in turn depends on various factors other than membrane deformability.

Membrane Deformability of Erythrocytes with Glucose-6-Phosphate Dehydrogenase Hamburg

1977 ◽  
Vol 57 (3) ◽  
pp. 162-167 ◽  
Author(s):  
W. Tillmann ◽  
M. Gahr ◽  
N. Labitzke ◽  
W. Schröter
Keyword(s):  
Deformability Of Erythrocytes ◽  
Glucose 6 Phosphate Dehydrogenase

Laser diffractometry of the surface of fatigue fractures under applied cyclic loads

1988 ◽  
Vol 20 (7) ◽  
pp. 972-976
Author(s):  
A. V. Matokhin ◽  
V. V. Yudin ◽  
G. V. Matokhin ◽  
T. Yu. Yakovleva ◽  
A. V. Khlamenok
Keyword(s):  
Cyclic Loads ◽  
Laser Diffractometry ◽  
Fatigue Fractures

scholarly journals PMCA activity and membrane tubulin affect deformability of erythrocytes from normal and hypertensive human subjects

2015 ◽  
Vol 1848 (11) ◽  
pp. 2813-2820 ◽  
Author(s):  
Noelia E. Monesterolo ◽  
Ayelen D. Nigra ◽  
Alexis N. Campetelli ◽  
Verónica S. Santander ◽  
Juan F. Rivelli ◽  
...  
Keyword(s):  
Human Subjects ◽  
Deformability Of Erythrocytes ◽  
Membrane Tubulin
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