scholarly journals Development of HRPzyme-Integrated PCR Platform for Colorimetric Detection of Foodborne Pathogens

2018 ◽  
Author(s):  
Bhagwan S. Batule ◽  
Seong U. Kim ◽  
Hyoyoung Mun ◽  
Won-Bo Shim ◽  
Min-Gon Kim
Keyword(s):  
Foodborne Pathogens ◽  
Colorimetric Detection

A microdevice for rapid, monoplex and colorimetric detection of foodborne pathogens using a centrifugal microfluidic platform

2018 ◽  
Vol 100 ◽  
pp. 96-104 ◽  
Author(s):  
Abkar Sayad ◽  
Fatimah Ibrahim ◽  
Shah Mukim Uddin ◽  
Jongman Cho ◽  
Marc Madou ◽  
...  
Keyword(s):  
Foodborne Pathogens ◽  
Colorimetric Detection ◽  
Microfluidic Platform

A finger-actuated microfluidic biosensor for colorimetric detection of foodborne pathogens

2021 ◽  
pp. 131801
Author(s):  
Wuzhen Qi ◽  
Lingyan Zheng ◽  
Yu Hou ◽  
Hong Duan ◽  
Lei Wang ◽  
...  
Keyword(s):  
Foodborne Pathogens ◽  
Colorimetric Detection ◽  
Microfluidic Biosensor

A foldable isothermal amplification microdevice for fuchsin-based colorimetric detection of multiple foodborne pathogens

Lab on a Chip ◽  
2019 ◽  
Vol 19 (8) ◽  
pp. 1397-1405 ◽  
Author(s):  
Thi Ngoc Diep Trinh ◽  
Nae Yoon Lee
Keyword(s):  
Foodborne Pathogens ◽  
Colorimetric Detection ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification

Loop-mediated isothermal amplification combined with fuchsin-based colorimetric detection in a foldable microdevice for rapid and multiplex identification of foodborne pathogens.

Development of a Paper-Based Analytical Device for Colorimetric Detection of Select Foodborne Pathogens

2012 ◽  
Vol 84 (6) ◽  
pp. 2900-2907 ◽  
Author(s):  
Jana C. Jokerst ◽  
Jaclyn A. Adkins ◽  
Bledar Bisha ◽  
Mallory M. Mentele ◽  
Lawrence D. Goodridge ◽  
...  
Keyword(s):  
Foodborne Pathogens ◽  
Colorimetric Detection ◽  
Analytical Device

Emerging foodborne pathogens

2006 ◽  
Author(s):  
Yasmine Motarjemi ◽  
Martin Adams
Keyword(s):  
Foodborne Pathogens

Directed Evolution of P450 Fatty Acid Decarboxylases via High-Throughput Screening Towards Improved Catalytic Activity

2019 ◽  
Author(s):  
Huifang Xu ◽  
Weinan Liang ◽  
Linlin Ning ◽  
Yuanyuan Jiang ◽  
Wenxia Yang ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Fatty Acid ◽  
Directed Evolution ◽  
High Throughput ◽  
High Throughput Screening ◽  
Colorimetric Detection ◽  
Screening Method ◽  
Random Mutagenesis ◽  
Direct Production ◽  
Consumption Activity

P450 fatty acid decarboxylases (FADCs) have recently been attracting considerable attention owing to their one-step direct production of industrially important 1-alkenes from biologically abundant feedstock free fatty acids under mild conditions. However, attempts to improve the catalytic activity of FADCs have met with little success. Protein engineering has been limited to selected residues and small mutant libraries due to lack of an effective high-throughput screening (HTS) method. Here, we devise a catalase-deficient <i>Escherichia coli</i> host strain and report an HTS approach based on colorimetric detection of H<sub>2</sub>O<sub>2</sub>-consumption activity of FADCs. Directed evolution enabled by this method has led to effective identification for the first time of improved FADC variants for medium-chain 1-alkene production from both DNA shuffling and random mutagenesis libraries. Advantageously, this screening method can be extended to other enzymes that stoichiometrically utilize H<sub>2</sub>O<sub>2</sub> as co-substrate.

Antioxidant and Antibacterial Activities of Artemisia herba-alba Asso Essential Oil from Middle Atlas, Morocco

2018 ◽  
Vol 16 (S1) ◽  
pp. S48-S54
Author(s):  
Y. Ez zoubi ◽  
S. Lairini ◽  
A. Farah ◽  
K. Taghzouti ◽  
A. El Ouali Lalami
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Foodborne Pathogens ◽  
Food Systems ◽  
Antibacterial Activities ◽  
Culture Collection ◽  
Bornyl Acetate ◽  
Bacterial Strains ◽  
Disk Diffusion Assay ◽  
Different Strains

The purpose of this study was to determine the chemical composition and to evaluate the antioxidant and antibacterial effects of the Moroccan Artemisia herba-alba Asso essential oil against foodborne pathogens. The essential oil of Artemisia herba-alba was analyzed by gas chromatography coupled with mass spectroscopy. The antibacterial activity was assessed against three bacterial strains isolated from foodstuff and three bacterial strains referenced by the ATCC (American Type Culture Collection) using the disk diffusion assay and the macrodilution method. The antioxidant activity was evaluated using the DPPH (2, 2-diphenyl-1- picrylhydrazyl) method. The fourteen compounds of the Artemisia herba-alba essential oil were identified; the main components were identified as β-thujone, chrysanthenone, α-terpineol, α-thujone, α-pinene, and bornyl acetate. The results of the antibacterial activity obtained showed a sensitivity of the different strains to Artemisia herba-alba essential oil with an inhibition diameter of 8.50 to 17.00 mm. Concerning the MICs (minimum inhibitory concentrations), the essential oil exhibited much higher antibacterial activity with MIC values of 2.5 μl/ml against Bacillus subtilis ATCC and Lactobacillus sp. The essential oil was found to be active by inhibiting free radicals with an IC50 (concentration of an inhibitor where the response is reduced by half) value of 2.9 μg/ml. These results indicate the possible use of the essential oil on food systems as an effective inhibitor of foodborne pathogens, as a natural antioxidant, and for potential pharmaceutical applications. However, further research is needed in order to determine the toxicity, antibacterial, and antioxidant effects in edible products.

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