A foldable isothermal amplification microdevice for fuchsin-based colorimetric detection of multiple foodborne pathogens

Lab on a Chip ◽  
2019 ◽  
Vol 19 (8) ◽  
pp. 1397-1405 ◽  
Author(s):  
Thi Ngoc Diep Trinh ◽  
Nae Yoon Lee
Keyword(s):  
Foodborne Pathogens ◽  
Colorimetric Detection ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification

Loop-mediated isothermal amplification combined with fuchsin-based colorimetric detection in a foldable microdevice for rapid and multiplex identification of foodborne pathogens.

scholarly journals Essential properties and pitfalls of colorimetric Reverse Transcription Loop-mediated Isothermal Amplification as a point-of-care test for SARS-CoV-2 diagnosis

2021 ◽  
Vol 27 (1) ◽  
Author(s):  
Bruna de Oliveira Coelho ◽  
Heloisa Bruna Soligo Sanchuki ◽  
Dalila Luciola Zanette ◽  
Jeanine Marie Nardin ◽  
Hugo Manuel Paz Morales ◽  
...  
Keyword(s):  
Viral Load ◽  
Internal Control ◽  
Reverse Transcription ◽  
Color Change ◽  
Point Of Care ◽  
Colorimetric Detection ◽  
False Negative ◽  
Isothermal Amplification ◽  
Clinical Samples ◽  
Loop Mediated Isothermal Amplification

Abstract Background SARS-CoV-2 Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP) colorimetric detection is a sensitive and specific point-of-care molecular biology technique used to detect the virus in only 30 min. In this manuscript we have described a few nuances of the technique still not properly described in the literature: the presence of three colors clusters; the correlation of the viral load with the color change; and the importance of using an internal control to avoid false-negative results. Methods To achieve these findings, we performed colorimetric RT-LAMP assays of 466 SARS-CoV-2 RT-qPCR validated clinical samples, with color quantification measured at 434 nm and 560 nm. Results First we determinate a sensitivity of 93.8% and specificity of 90.4%. In addition to the pink (negative) and yellow (positive) produced colors, we report for the first time the presence of an orange color cluster that may lead to wrong diagnosis. We also demonstrated using RT-qPCR and RT-LAMP that low viral loads are related to Ct values > 30, resulting in orange colors. We also demonstrated that the diagnosis of COVID-19 by colorimetric RT-LAMP is efficient until the fifth symptoms day when the viral load is still relatively high. Conclusion This study reports properties and indications for colorimetric RT-LAMP as point-of-care for SARS-CoV-2 diagnostic, reducing false results, interpretations and optimizing molecular diagnostics tests application.

scholarly journals Colorimetric Detection of Dengue by Single Tube Reverse-Transcription-Loop-Mediated Isothermal Amplification

PLoS ONE ◽  
2015 ◽  
Vol 10 (9) ◽  
pp. e0138694 ◽  
Author(s):  
Yee-Ling Lau ◽  
Meng-Yee Lai ◽  
Boon-Teong Teoh ◽  
Juraina Abd-Jamil ◽  
Jefree Johari ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Colorimetric Detection ◽  
Isothermal Amplification ◽  
Single Tube ◽  
Loop Mediated Isothermal Amplification

Loop-mediated isothermal amplification technique: principle, development and wide application in food safety

2020 ◽  
Vol 12 (46) ◽  
pp. 5551-5561
Author(s):  
Tianzeng Huang ◽  
Linzhi Li ◽  
Xing Liu ◽  
Qi Chen ◽  
Xueen Fang ◽  
...  
Keyword(s):  
Food Safety ◽  
Gene Amplification ◽  
Foodborne Pathogens ◽  
High Specificity ◽  
Isothermal Amplification ◽  
Isothermal Conditions ◽  
Loop Mediated Isothermal Amplification ◽  
Novel Gene ◽  
Amplification Method ◽  
Amplification Technique

LAMP is a relatively novel gene amplification method under isothermal conditions with rapidity, and high specificity. It is widely applied in the field of food safety, such as in the detection of foodborne pathogens, GM, OP pesticides and so on

On-chip parallel detection of foodborne pathogens using loop-mediated isothermal amplification

2013 ◽  
Vol 15 (5) ◽  
pp. 821-830 ◽  
Author(s):  
Carlos Duarte ◽  
Eric Salm ◽  
Brian Dorvel ◽  
Bobby Reddy ◽  
Rashid Bashir
Keyword(s):  
Foodborne Pathogens ◽  
Isothermal Amplification ◽  
Parallel Detection ◽  
Loop Mediated Isothermal Amplification ◽  
On Chip

scholarly journals Specific and sensitive, ready-to-use universal fungi detection by visual color using ITS1 loop-mediated isothermal amplification combined hydroxynaphthol blue

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11082
Author(s):  
Ilada Choopara ◽  
Yothin Teethaisong ◽  
Narong Arunrut ◽  
Sudaluck Thunyaharn ◽  
Wansika Kiatpathomchai ◽  
...  
Keyword(s):  
Point Of Care ◽  
Colorimetric Detection ◽  
High Sensitivity ◽  
Fungal Species ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
Fungal Isolates ◽  
Simple Detection ◽  
Hydroxynaphthol Blue ◽  
Life Threatening

Being ubiquitous, fungi are common opportunistic pathogens to humans that can lead to invasive and life-threatening infections in immunocompromised individuals. Eukaryote-resembling cell membrane and filamentous branches make the fungal diagnosis difficult. This study therefore developed a ready-to-use ITS1 loop-mediated isothermal amplification combined with hydroxynaphthol blue (LAMP-HNB) for rapid, sensitive and specific colorimetric detection of universal fungi in all phyla. The ITS1 LAMP-HNB could identify every evolutionary phylum of fungi according to sequence analyses. We tested a total of 30 clinically relevant fungal isolates (representing three major human pathogenic phyla of fungi, namely Zygomycota, Ascomycota and Basidiomycota) and 21 non-fungal isolates, and the ITS1 LAMP-HNB properly identified all isolates, with a detection limit of as low as 4.6 ag (9.6 copies), which was identical to ITS1 and 18S rDNA PCR. The assays were also validated on the feasibility of point-of-care diagnostic with real food (dry peanuts, chili and garlics) and blood samples. Furthermore, the shelf life of our ready-to-use ITS1 LAMP activity (≥50%) was more than 40 days at 30 °C with 3–5% polyvinyl alcohol or glycerol additive. The results supported the ready-to-use ITS1 LAMP-HNB for simple detection of fungi contamination with high sensitivity in local and resource-constrained areas to prevent opportunistic fungal species infections.

Sign in / Sign up

Export Citation Format

Share Document