Development of a Paper-Based Analytical Device for Colorimetric Detection of Select Foodborne Pathogens

2012 ◽  
Vol 84 (6) ◽  
pp. 2900-2907 ◽  
Author(s):  
Jana C. Jokerst ◽  
Jaclyn A. Adkins ◽  
Bledar Bisha ◽  
Mallory M. Mentele ◽  
Lawrence D. Goodridge ◽  
...  
Keyword(s):  
Foodborne Pathogens ◽  
Colorimetric Detection ◽  
Analytical Device

scholarly journals Molecular Diagnostic of Ochratoxin A with Specific Aptamers in Corn and Groundnut via Fabrication of A Microfluidic Device

2021 ◽  
Author(s):  
Deepshikha Shahdeo ◽  
Azmat Ali Khan ◽  
Amer M Alanazi ◽  
Yun Suk Huh ◽  
Shruti Shukla ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Limit Of Detection ◽  
Colorimetric Assay ◽  
Animal Health ◽  
Colorimetric Detection ◽  
Molecular Diagnostic ◽  
Detection Range ◽  
Vis Spectroscopy ◽  
Wide Range ◽  
Analytical Device

Abstract Ochratoxin A (OTA) is one of the predominant mycotoxins that contaminate a wide range of food commodities. In the present study, a 36-mer aptamer was used as a molecular recognition element coupled with gold nanoparticles (AuNPs) for colorimetric detection of OTA in a microfluidic paper-based analytical device (µPADs). The µPADs consisted of three zones: control, detection, and sample, interconnected by channels. The biophysical characterizations of aptamer conjugated AuNPs were done by UV-vis spectroscopy (UV-vis), dynamic Light Scattering (DLS), and transmission electron microscopy (TEM). The developed colorimetric assay for OTA showed a limit of detection of 242, 545, and 95.69 ng/mL in water, corn, and groundnut, respectively. The HPLC detection method achieved acceptable coefficient in standard curves (r2 = 0.9995), better detection range, and recovery rates in spiked corn and groundnut samples as 43.61 ± 2.18% to 87.10 ± 1.82% and 42.01 ± 1.31% to 86.03 ± 2.64% after multiple sample extractions and cleanup steps. However, the developed µPADs analytical device had the potent ability to rapidly detect OTA without any extraction pre-requirement, derivatization, and cleanup steps, thus illustrating its feasibility in the animal health sector, agricultural, and food industries.

scholarly journals A Microfluidic Paper-Based Analytical Device for Type-II Pyrethroid Targets in an Environmental Water Sample

Sensors ◽  
2020 ◽  
Vol 20 (15) ◽  
pp. 4107 ◽  
Author(s):  
Sumate Pengpumkiat ◽  
Jintana Nammoonnoy ◽  
Watcharaporn Wongsakoonkan ◽  
Pajaree Konthonbut ◽  
Pornpimol Kongtip
Keyword(s):  
Water Sample ◽  
Detection System ◽  
Low Cost ◽  
Colorimetric Detection ◽  
Environmental Water ◽  
Type Ii ◽  
Environmental Water Sample ◽  
Detection Approach ◽  
Hydrophobic Barrier ◽  
Analytical Device

A detection method for type-II pyrethroids in an environmental water sample using a microfluidic paper-based analytical device (µPAD) is reported here. The detection approach is based on the formation of cyanide from the hydrolysis of type-II pyrethroids and the colorimetric detection of cyanide on a layer-based µPAD. Parafilm and inexpensive laminating pouches were used to create a hydrophobic barrier for the assay on the µPAD. This detection approach was selective to type-II pyrethroids in water for which an environmental water sample was tested. The calibration curves for cypermethrin, deltamethrin, cyhalothrin, and fenvalerate ranged from 2 to 40 µg/mL without sample preconcentration. The lower concentrations of type-II pyrethroids can be assessed by including a preconcentration step prior to the detection on a µPAD. This detection system provides an alternative platform for fast, semiquantitative testing for pesticide contamination in environmental surface water by allowing for portability, low reagent/sample consumption, and low-cost testing.

scholarly journals Use of plastic-based analytical device, smartphone and chemometric tools to discriminate amines

RSC Advances ◽  
2015 ◽  
Vol 5 (26) ◽  
pp. 20148-20154 ◽  
Author(s):  
Lígia Bueno ◽  
Gabriel N. Meloni ◽  
Subrayal M. Reddy ◽  
Thiago R. L. C. Paixão
Keyword(s):  
Volatile Compounds ◽  
Foodborne Pathogens ◽  
Diagnostic Approach ◽  
Chemometric Tools ◽  
Analytical Device

Amine-based volatile compounds released by microorganisms offer an alternative diagnostic approach for the identification of foodborne pathogens.

A novel, simple and low-cost paper-based analytical device for colorimetric detection of Cronobacter spp.

2018 ◽  
Vol 1036 ◽  
pp. 80-88 ◽  
Author(s):  
Luhong Sun ◽  
Yujun Jiang ◽  
Ruili Pan ◽  
Mingyu Li ◽  
Rui Wang ◽  
...  
Keyword(s):  
Low Cost ◽  
Colorimetric Detection ◽  
Analytical Device

A microdevice for rapid, monoplex and colorimetric detection of foodborne pathogens using a centrifugal microfluidic platform

2018 ◽  
Vol 100 ◽  
pp. 96-104 ◽  
Author(s):  
Abkar Sayad ◽  
Fatimah Ibrahim ◽  
Shah Mukim Uddin ◽  
Jongman Cho ◽  
Marc Madou ◽  
...  
Keyword(s):  
Foodborne Pathogens ◽  
Colorimetric Detection ◽  
Microfluidic Platform

scholarly journals Development of a paper-based analytical device for colorimetric detection of uric acid using gold nanoparticles–graphene oxide (AuNPs–GO) conjugates

2016 ◽  
Vol 8 (38) ◽  
pp. 6965-6973 ◽  
Author(s):  
Sanjay Kumar ◽  
Pulak Bhushan ◽  
Shantanu Bhattacharya
Keyword(s):  
Gold Nanoparticles ◽  
Uric Acid ◽  
Graphene Oxide ◽  
Colorimetric Detection ◽  
Analytical Device

A novel paper-based device for colorimetric detection of uric acid was developed based on the peroxidase-like activity of gold nanoparticles–graphene conjugates (AuNPs–GO).

Paper-based analytical device for colorimetric detection of Cu2+ in Brazilian sugarcane spirits by digital image treatment

2020 ◽  
Vol 159 ◽  
pp. 105463
Author(s):  
Gabriel M. Fernandes ◽  
Júlia de Sousa ◽  
João Flávio da Silveira Petruci ◽  
Alex D. Batista
Keyword(s):  
Digital Image ◽  
Colorimetric Detection ◽  
Analytical Device
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