scholarly journals High Sensitivity Photodetector for Photon-Counting Applications

2018 ◽  
Author(s):  
Fabio Acerbi ◽  
Matteo Perenzoni
Keyword(s):  
Photon Counting ◽  
High Sensitivity

scholarly journals Enhanced chemiluminescence: a high-sensitivity detection system for in situ hybridization and immunohistochemistry.

1993 ◽  
Vol 41 (11) ◽  
pp. 1591-1597 ◽  
Author(s):  
P Lorimier ◽  
L Lamarcq ◽  
F Labat-Moleur ◽  
C Guillermet ◽  
R Bethier ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Detection System ◽  
Photon Counting ◽  
Protein Detection ◽  
High Sensitivity ◽  
Papilloma Virus ◽  
Enhanced Chemiluminescence ◽  
Discriminatory Analysis ◽  
High Sensitivity Detection

The breakthrough of chemiluminescence in the field of solution immunoassays and transfer membranes prompted us to explore whether a light-based detection system could provide a gain in sensitivity over chromogenic and FITC markers for nucleic acid and protein detection on histological preparations. A Hamamatsu device and an enhanced chemiluminescence (ECL) luminol substrate of the peroxidase were used to detect epithelial and endothelial components by immunohistochemistry (IHC) and for in situ hybridization (ISH) of papilloma virus DNA. The accuracy of the signal was compared to that obtained with DAB-peroxidase, silver-enhanced DAB-peroxidase, NBT-BCIP-alkaline phosphatase, and FITC. Our results demonstrated the feasibility and high sensitivity of luminescence detection for histological preparations. In part due to the ultrasensitive videocamera and photon-counting imaging, interpretable and reproducible results were obtained within counting times shorter than 5 min, and with dilutions of the primary antibodies 100- to 10,000-fold greater than those used for chromogenic and FITC reactions. As for ISH, with identical concentrations of the HPV 18 DNA probe on HeLa cells, labeling was apparent by luminescence but undetectable with the chromogen. The morphological resolution allowed a discriminatory analysis of the signal. Therefore, at the light microscopic level, enhanced chemiluminescence offers an appealing alternative to FITC and chromogenic markers for detection and quantification of low-concentration molecules.

High-sensitivity photon-counting imaging detector

2005 ◽  
Author(s):  
Lichen Fu ◽  
Ye Li ◽  
Qingduo Duanmu ◽  
Guozheng Wang ◽  
Kui Wu ◽  
...  
Keyword(s):  
Photon Counting ◽  
High Sensitivity ◽  
Imaging Detector

scholarly journals Characterization and performance evaluation of the XSPA-500k detector using synchrotron radiation

2021 ◽  
Vol 28 (2) ◽  
pp. 439-447
Author(s):  
Yasukazu Nakaye ◽  
Takuto Sakumura ◽  
Yasutaka Sakuma ◽  
Satoshi Mikusu ◽  
Arkadiusz Dawiec ◽  
...  
Keyword(s):  
High Performance ◽  
Performance Metrics ◽  
Dynamic Range ◽  
Photon Counting ◽  
High Sensitivity ◽  
High Dynamic Range ◽  
Frame Rate ◽  
X Rays ◽  
Response Modulation ◽  
Modulation Transfer

Hybrid photon counting (HPC) detectors are widely used at both synchrotron facilities and in-house laboratories. The features of HPC detectors, such as no readout noise, high dynamic range, high frame rate, excellent point spread function, no blurring etc. along with fast data acquisition, provide a high-performance detector with a low detection limit and high sensitivity. Several HPC detector systems have been developed around the world. A number of them are commercially available and used in academia and industry. One of the important features of an HPC detector is a fast readout speed. Most HPC detectors can easily achieve over 1000 frames s−1, one or two orders of magnitude faster than conventional CCD detectors. Nevertheless, advanced scientific challenges require ever faster detectors in order to study dynamical phenomena in matter. The XSPA-500k detector can achieve 56 kframes s−1 continuously, without dead-time between frames. Using `burst mode', a special mode of the UFXC32k ASIC, the frame rate reaches 1 000 000 frames s−1. XSPA-500k was fully evaluated at the Metrology beamline at Synchrotron SOLEIL (France) and its readout speed was confirmed by tracking the synchrotron bunch time structure. The uniformity of response, modulation transfer function, linearity, energy resolution and other performance metrics were also verified either with fluorescence X-rays illuminating the full area of the detector or with the direct beam.

scholarly journals Multi-speckle X-ray photon correlation spectroscopy in the ultra-small-angle X-ray scattering range

2016 ◽  
Vol 23 (4) ◽  
pp. 929-936 ◽  
Author(s):  
Johannes Möller ◽  
Yuriy Chushkin ◽  
Sylvain Prevost ◽  
Theyencheri Narayanan
Keyword(s):  
Small Angle ◽  
Photon Correlation Spectroscopy ◽  
Photon Counting ◽  
High Sensitivity ◽  
Correlation Spectroscopy ◽  
Colloidal Systems ◽  
Photon Correlation ◽  
Length Scales ◽  
X Ray ◽  
New Research

Multi-speckle X-ray photon correlation spectroscopy (XPCS) measurements in the ultra-small-angle range are performed using a long pinhole collimation instrument in combination with two-dimensional photon-counting and high-sensitivity imaging detectors. The feasibility of the presented setup to measure dynamics on different time and length scales pertinent to colloidal systems is shown. This setup offers new research opportunities, such as for example in the investigation of non-equilibrium dynamics in optically opaque, complex systems over length scales from tens of nanometres to several micrometres. In addition, due to the short duration of the X-ray exposure involved in the ultra-small-angle range, possible radiation-induced effects are alleviated. Furthermore, the performance of two different detectors, a photon-counting Pilatus 300K and an integrating FReLoN CCD, are compared, and their applicability for accurate XPCS measurements is demonstrated.

Study on Initial Growth of Particles in Low-Pressure and Low-Power GeH4 RF Discharges Using the High-Sensitivity Photon-Counting Laser-Light-Scattering Method

1998 ◽  
Vol 37 (Part 2, No. 10B) ◽  
pp. L1264-L1267 ◽  
Author(s):  
Hiroharu Kawasaki ◽  
Kazutaka Sakamoto ◽  
Shinichi Maeda ◽  
Tsuyoshi Fukuzawa ◽  
Masaharu Shiratani ◽  
...  
Keyword(s):  
Light Scattering ◽  
Low Power ◽  
Laser Light ◽  
Photon Counting ◽  
High Sensitivity ◽  
Low Pressure ◽  
Laser Light Scattering ◽  
Scattering Method ◽  
Initial Growth ◽  
Rf Discharges

High Sensitivity Fluorophotometer Using Photon Counting

1973 ◽  
Vol 12 (11) ◽  
pp. 2748 ◽  
Author(s):  
Reiji Aoshima ◽  
Keiji Iriyama ◽  
Hiroshi Asai
Keyword(s):  
Photon Counting ◽  
High Sensitivity
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