Colloids: Applications in Sperm Preparation for Assisted Reproduction

PP-4 USE OF PROINSERT™ SPERM PREPARATION FOR DISCORDANT HIV COUPLES UNDERGOING ASSISTED REPRODUCTION

Reproductive BioMedicine Online ◽

10.1016/s1472-6483(12)60136-9 ◽

2012 ◽

Vol 24 ◽

pp. S6

Author(s):

Jacquelyn Irving ◽

Barbara Hall ◽

Karen Watson ◽

Marianne Ilbery ◽

Keith Harrison

Keyword(s):

Assisted Reproduction ◽

Sperm Preparation

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Effect of sperm preparation on development of bovine blastocyst in vitro

Zygote ◽

10.1017/s0967199416000150 ◽

2016 ◽

Vol 24 (6) ◽

pp. 825-830 ◽

Cited By ~ 9

Author(s):

Maria Celina Abraham ◽

Anders Johannisson ◽

Jane M. Morrell

Keyword(s):

Assisted Reproduction ◽

Membrane Integrity ◽

Single Layer ◽

Human Sperm ◽

Sperm Chromatin ◽

Computer Assisted ◽

Cleavage Rate ◽

Preparation Methods ◽

Sperm Preparation

SummarySperm preparation is an important step in the in vitro production of embryos. Centrifugation through colloids has been used to select normal sperm for assisted reproduction in several species. Animal models can sometimes be used as a preliminary step to investigate sperm preparation methods that are potentially of use for human fertility treatments. In this study bovine semen was prepared using three variants of the single-layer centrifugation sperm selection technique (Small, Mini, Mini-EP) with Bovicoll (Androcoll-B). Computer-assisted sperm motility analysis, the hypo-osmotic swelling test, and the sperm chromatin structure assay were performed on unselected (control) and SLC-selected sperm samples. Mini and Mini-EP gave the highest yield of motile spermatozoa, progressive motility and membrane integrity. In vitro fertilization trials were performed to investigate the fertilizing ability of the frozen–thawed bovine spermatozoa selected with Bovicoll. Mini-SLC (single-layer centrifugation) and swim-up (Control) were performed and cleavage rate and blastocyst rate did not differ significantly between groups. As there was a trend to an increased number of cells in blastocysts in the SLC group, the Mini-SLC method is at least as good as swim-up for selecting frozen–thawed bull spermatozoa for in vitro fertilization (IVF). This method could potentially be used to prepare human sperm for assisted reproduction.

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