scholarly journals Prospective controlled trial of an electrophoretic method of sperm preparation for assisted reproduction: comparison with density gradient centrifugation

2008 ◽  
Vol 23 (12) ◽  
pp. 2646-2651 ◽  
Author(s):  
S.D. Fleming ◽  
R.S. Ilad ◽  
A-M.G. Griffin ◽  
Y. Wu ◽  
K.J. Ong ◽  
...  
Keyword(s):  
Density Gradient ◽  
Assisted Reproduction ◽  
Density Gradient Centrifugation ◽  
Controlled Trial ◽  
Gradient Centrifugation ◽  
Electrophoretic Method ◽  
Sperm Preparation

scholarly journals Sperm quality after density gradient centrifugation with three commercially available media: a controlled trial

2014 ◽  
Vol 12 (1) ◽  
pp. 121 ◽  
Author(s):  
Helena Malvezzi ◽  
Rakesh Sharma ◽  
Ashok Agarwal ◽  
Adel M Abuzenadah ◽  
Muhammad Abu-Elmagd
Keyword(s):  
Density Gradient ◽  
Density Gradient Centrifugation ◽  
Sperm Quality ◽  
Controlled Trial ◽  
Gradient Centrifugation

scholarly journals Sperm Selection and Embryo Development: A Comparison of the Density Gradient Centrifugation and Microfluidic Chip Sperm Preparation Methods in Patients with Astheno-Teratozoospermia

Life ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 933
Author(s):  
Cagla Guler ◽  
Sureyya Melil ◽  
Umit Ozekici ◽  
Yaprak Donmez Cakil ◽  
Belgin Selam ◽  
...  
Keyword(s):  
Density Gradient ◽  
Embryo Development ◽  
Microfluidic Chip ◽  
Density Gradient Centrifugation ◽  
Gradient Centrifugation ◽  
Sperm Concentration ◽  
Gradient Methods ◽  
Semen Parameters ◽  
Preparation Methods ◽  
Sperm Preparation

In recent years, microfluidic chip-based sperm sorting has emerged as an alternative tool to centrifugation-based conventional techniques for in vitro fertilization. This prospective study aims to compare the effects of density gradient centrifugation and microfluidic chip sperm preparation methods on embryo development in patient populations with astheno-teratozoospermia. In the study, the semen samples of the patients were divided into two groups for preparation with either the microfluidic or density gradient methods. Selected spermatozoa were then used to fertilize mature sibling oocytes and the semen parameters and embryo development on days 3 and 5 were assessed. While the density gradient group was associated with a higher sperm concentration, motility (progressive and total) was significantly higher in the microfluidic chip group. No significant differences were observed in the fertilization rates or grade 1 (G1) and grade 2 (G2) proportions of the third-day embryos. Furthermore, while the proportions of the poor, fair and good blastocysts on day 5 did not differ significantly, excellent blastocysts (indicating high-quality embryos) were observed in a significantly higher proportion of the microfluidic chip group. When compared to the classical density gradient method, the microfluidic chip sperm preparation yielded sperm with higher motility and higher quality blastocysts at day 5; in patients with astheno-teratozoospermia.

scholarly journals Effect of Osmolarity and Density of Colloid Formulations on the Outcome of SLC-Selection of Stallion Spermatozoa

2011 ◽  
Vol 2011 ◽  
pp. 1-5 ◽  
Author(s):  
J. M. Morrell ◽  
A. Johannisson ◽  
H. Rodriguez-Martinez
Keyword(s):  
Density Gradient ◽  
Assisted Reproduction ◽  
Density Gradient Centrifugation ◽  
Sperm Quality ◽  
Gradient Centrifugation ◽  
Single Layer ◽  
Normal Density ◽  
High Osmolarity ◽  
Sperm Survival ◽  
Selection Of

The osmolarity and density of colloids used to prepare spermatozoa for assisted reproduction may affect sperm quality in the resultant preparation. In this study, two osmolarities of Androcoll-E for single-layer or density gradient centrifugation of stallion spermatozoa were compared: “normal” (320 mOsm) or “high” (345 mOsm). Mean yields for the two centrifugation techniques did not differ between treatments or osmolarities (single layer centrifugation: 30.19±16.9×106 and 25.8±18.5×106 spermatozoa; density gradient centrifugation: 31.84±19.7×106 and 26.46±20.0×106 spermatozoa respectively for the two osmolarities). However, use of the high osmolarity colloid for single layer centrifugation increased the proportion of morphologically normal spermatozoa (P<.05). Therefore, increasing the osmolarity of the colloid formulation may be beneficial for processing ejaculates containing a high proportion of abnormal spermatozoa by SLC. Reducing the density of the colloid used for the SLC substantially increased the yield of motile spermatozoa compared to the normal density colloid (mean ± SD: 72.6±28.9×106 versus 28.9±24.7×106), while also prolonging sperm survival by 24 hours compared to the uncentrifuged ejaculate. This increased yield may render Single Layer Centrifugation practical for use in the field.

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