scholarly journals The Potential of Genetically Engineered Magnetic Particles in Biomedical Applications

10.5772/22750 ◽  
2011 ◽  
Author(s):  
Tomoko Yoshino ◽  
Yuka Kanetsuki ◽  
Tadashi Matsunag
Keyword(s):  
Biomedical Applications ◽  
Magnetic Particles ◽  
Genetically Engineered

scholarly journals How to Improve Physico-Chemical Properties of Silk Fibroin Materials for Biomedical Applications?—Blending and Cross-Linking of Silk Fibroin—A Review

Materials ◽  
2021 ◽  
Vol 14 (6) ◽  
pp. 1510
Author(s):  
Sylwia Grabska-Zielińska ◽  
Alina Sionkowska
Keyword(s):  
Silk Fibroin ◽  
Biomedical Applications ◽  
Magnetic Particles ◽  
Chemical Properties ◽  
Ternary Mixtures ◽  
Cross Linking ◽  
Advantages And Disadvantages ◽  
Physico Chemical ◽  
Binary And Ternary Mixtures ◽  
To Receive

This review supplies a report on fresh advances in the field of silk fibroin (SF) biopolymer and its blends with biopolymers as new biomaterials. The review also includes a subsection about silk fibroin mixtures with synthetic polymers. Silk fibroin is commonly used to receive biomaterials. However, the materials based on pure polymer present low mechanical parameters, and high enzymatic degradation rate. These properties can be problematic for tissue engineering applications. An increased interest in two- and three-component mixtures and chemically cross-linked materials has been observed due to their improved physico-chemical properties. These materials can be attractive and desirable for both academic, and, industrial attention because they expose improvements in properties required in the biomedical field. The structure, forms, methods of preparation, and some physico-chemical properties of silk fibroin are discussed in this review. Detailed examples are also given from scientific reports and practical experiments. The most common biopolymers: collagen (Coll), chitosan (CTS), alginate (AL), and hyaluronic acid (HA) are discussed as components of silk fibroin-based mixtures. Examples of binary and ternary mixtures, composites with the addition of magnetic particles, hydroxyapatite or titanium dioxide are also included and given. Additionally, the advantages and disadvantages of chemical, physical, and enzymatic cross-linking were demonstrated.

Viscoelastic Properties of Genetically Engineered Silk-Elastin-Like Protein Polymers

2008 ◽  
Author(s):  
Weibing Teng ◽  
Joseph Cappello ◽  
Xiaoyi Wu
Keyword(s):  
Drug Delivery ◽  
Viscoelastic Properties ◽  
Biomedical Applications ◽  
Biological Properties ◽  
Genetically Engineered ◽  
Structural Proteins ◽  
Design And Synthesis ◽  
Cross Links ◽  
New Protein ◽  
Polypeptide Sequences

Genetic engineering of protein-based materials provides material scientists with high levels of control in material microstructures, properties, and functions [1]. For example, multi-block protein copolymers in which individual block may possess distinct mechanical or biological properties have been biosynthesized [2, 3]. Polypeptide sequences derived from well-studied structural proteins (e.g., collagen, silk, elastin) are often used as motifs in the design and synthesis of new protein-based material, in which new functional groups may be incorporated. In this fashion, we have produced a series of silk-elastin-like proteins (SELPs) consisting of polypeptide sequences derived from silk of superior mechanical strength and elastin that is extremely durable and resilient [2, 4]. Notably, the silk-like blocks are capable of crystallizing to form virtual cross-links between elastin-mimetic sequences, which, in turn, lower the crystallinity of the silk-like blocks and thus enhance the solubility of SELPs. Consequently, SELPs may be fabricated into useful structures for biomedical applications, including drug delivery. In this study, we will characterize viscoelastic properties of SELPs, which are particularly relevant to tissue engineering applications.

Synthesis of Biocompatible Magnetic Iron Oxide (γ-Fe2O3 and Fe3O4) Nanoparticles by a Modified Polyol Process for Biomedical Applications

2010 ◽  
Vol 1256 ◽  
Author(s):  
Georgia Basina ◽  
Ioannis Panagiotopoulos ◽  
Eamonn Devlin ◽  
George Hadjipanayis ◽  
Levent Colak ◽  
...  
Keyword(s):  
Biomedical Applications ◽  
Magnetic Particles ◽  
Polyol Process ◽  
X Rays ◽  
Colloidal Solutions ◽  
Heating Rates ◽  
Fast Heating ◽  
Ft Ir ◽  
Magnetite Phase ◽  
Ft Ir Spectroscopy

AbstractHighly crystalline superparamagnetic Fe3O4 nanoparticles coated by poly-vinylpyrrolidone (PVP) were prepared by simultaneous thermal decomposition of ferrous and ferric inorganic salts in polyethylene glycol (PEG) with molecular weight 200. The magnetic particles have a diameter in the range of 8-15 nm, and after exchange with citric acid diammonium salt, they transform into very stable super hydrophilic colloidal solutions. The presence of magnetite phase was confirmed using powder X-rays diffraction (XRD) and Mössbauer spectroscopy, while thermogravimetric analysis and FT-IR spectroscopy confirmed the presence of PVP or citrate anions on the nanoparticles surface. The magnetic properties revealed superparamagnetic behavior, with the composite material showing a saturation magnetization up to 57 emu/g. The Fe3O4 nanoparticles prepared by this modified polyol process are suitable for biomedical applications because of the biocompatibility of citrate anions. Magnetic hyperthermia experiments in neutral water solutions shows that the particles induce fast heating rates with specific absorption rate (SAR) values which reached 57.53 W/gFe, when the concentration of iron is 11.2 mgFe/ml.

Sinusoidal magnetic field stimulates magnetosome formation and affects mamA, mms13, mms6, and magA expression in Magnetospirillum magneticum AMB-1

2008 ◽  
Vol 54 (12) ◽  
pp. 1016-1022 ◽  
Author(s):  
Xiaoke Wang ◽  
Likun Liang ◽  
Tao Song ◽  
Longfei Wu
Keyword(s):  
Magnetic Field ◽  
Geomagnetic Field ◽  
Biomedical Applications ◽  
Magnetic Particles ◽  
Industrial Sector ◽  
Magnetic Pole ◽  
Variable Intensity ◽  
New Approach ◽  
Sinusoidal Magnetic Field ◽  
Magnetospirillum Magneticum

Magnetic particles are currently one of the most important materials in the industrial sector, where they have been widely used for biotechnological and biomedical applications. To investigate the effects of the imposed magnetic field on biomineralization in Magnetospirillum magneticum AMB-1 and to suggest a new approach that enhances formation of magnetosomes, cultures inoculated with either magnetic or nonmagnetic precultures were incubated under a sinusoidal magnetic field or geomagnetic field. The results showed that the sinusoidal magnetic field up-regulated mms6 expression in the cultures inoculated with magnetic cells, and magA, mms6, and mamA expression in the cultures inoculated with nonmagnetic cells. The applied sinusoidal magnetic field could block cell division, which could contribute to a decrease in the OD600 values and an increase in the coefficient of magnetism values of the cultures, which could mean that the percentage of mature magnetosome-containing bacteria was increased. The linearity of magnetosome chains was affected, but the number of magnetic particles in cells was increased when a sinusoidal magnetic field was applied to the cultures. The results imply that the variable intensity and orientation of the sinusoidal magnetic field resulted in magnetic pole conversion in the newly forming magnetic particles, which could affect the formation of magnetic crystals and the arrangement of the adjacent magnetosome.

Commercialising genetically engineered animal biomedical products

2008 ◽  
Vol 20 (1) ◽  
pp. 61 ◽  
Author(s):  
Eddie J. Sullivan ◽  
Jerry Pommer ◽  
James M. Robl
Keyword(s):  
Biomedical Applications ◽  
Gap Analysis ◽  
Early Stage ◽  
Genetically Engineered ◽  
The Public ◽  
Public Benefit ◽  
The Past ◽  
Cell Banking ◽  
Stage Development ◽  
Founder Animal

Research over the past two decades has increased the quality and quantity of tools available to produce genetically engineered animals. The number of potentially viable biomedical products from genetically engineered animals is increasing. However, moving from cutting-edge research to development and commercialisation of a biomedical product that is useful and wanted by the public has significant challenges. Even early stage development of genetically engineered animal applications requires consideration of many steps, including quality assurance and quality control, risk management, gap analysis, founder animal establishment, cell banking, sourcing of animals and animal-derived material, animal facilities, product collection facilities and processing facilities. These steps are complicated and expensive. Biomedical applications of genetically engineered animals have had some recent successes and many applications are well into development. As researchers consider applications for their findings, having a realistic understanding of the steps involved in the development and commercialisation of a product, produced in genetically engineered animals, is useful in determining the risk of genetic modification to the animal v. the potential public benefit of the application.

Fabrication of Genetically Engineered Silk-Elastin-Like Protein Polymer Fibers

2008 ◽  
Author(s):  
Weiguo Qiu ◽  
Joseph Cappello ◽  
Xiaoyi Wu
Keyword(s):  
Tissue Engineering ◽  
Biomedical Applications ◽  
Recombinant Dna ◽  
Building Blocks ◽  
Genetically Engineered ◽  
Polymer Fibers ◽  
Structural Support ◽  
Protein Polymer ◽  
Protein Fibers ◽  
Cellular Matrices

Micro- and submicro-diameter protein fibers are fundamental building blocks of extra- and intra-cellular matrices, providing structural support, stability and protection to cells, tissues and organism [1]. Fabricating performance fibers of both naturally derived and genetically engineered proteins has been extensively pursued for a variety of biomedical applications, including tissue engineering and drug delivery [2]. Silk-elastin-like proteins (SELPs), consisting of tandemly repeated polypeptide sequences derived from silk and elastin, have been biosynthesized using recombinant DNA technique [3]. Their potential as a biomaterials in the form of hydrogels continues to be explored [4, 5]. This study will focus on the fabrication of robust, micro-diameter SELP fibers as biomaterials for tissue engineering applications.

Biomedical applications of mesoscale magnetic particles

MRS Bulletin ◽  
2013 ◽  
Vol 38 (11) ◽  
pp. 927-932 ◽  
Author(s):  
Bettina Kozissnik ◽  
Jon Dobson
Keyword(s):  
Biomedical Applications ◽  
Magnetic Particles ◽  
Image Position

Abstract

scholarly journals Polysaccharide-Coated Magnetic Nanoparticles for Imaging and Gene Therapy

2015 ◽  
Vol 2015 ◽  
pp. 1-14 ◽  
Author(s):  
Saji Uthaman ◽  
Sang Joon Lee ◽  
Kondareddy Cherukula ◽  
Chong-Su Cho ◽  
In-Kyu Park
Keyword(s):  
Magnetic Nanoparticles ◽  
Biomedical Applications ◽  
Magnetic Particles ◽  
Metal Oxide Nanoparticles ◽  
Vital Role ◽  
Imaging Characteristics ◽  
Magnetic Metal ◽  
Therapeutic Properties ◽  
The Many ◽  
Imaging Properties

Today, nanotechnology plays a vital role in biomedical applications, especially for the diagnosis and treatment of various diseases. Among the many different types of fabricated nanoparticles, magnetic metal oxide nanoparticles stand out as unique and useful tools for biomedical applications, because of their imaging characteristics and therapeutic properties such as drug and gene carriers. Polymer-coated magnetic particles are currently of particular interest to investigators in the fields of nanobiomedicine and fundamental biomaterials. Theranostic magnetic nanoparticles that are encapsulated or coated with polymers not only exhibit imaging properties in response to stimuli, but also can efficiently deliver various drugs and therapeutic genes. Even though a large number of polymer-coated magnetic nanoparticles have been fabricated over the last decade, most of these have only been used for imaging purposes. The focus of this review is on polysaccharide-coated magnetic nanoparticles used for imaging and gene delivery.

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