scholarly journals Complementary Control of Intermittently Operating Renewable Sources with Short- and Long-Term Storage Plants

10.5772/17662 ◽  
2011 ◽  
Author(s):  
E. F. ◽  
W. L.
Keyword(s):  
Renewable Sources ◽  
Long Term Storage ◽  
Short And Long Term ◽  
Term Storage

Short- and Long-Term Storage of Gametes

2016 ◽  
pp. 53-60
Author(s):  
Daniel Żarski ◽  
Ákos Horváth ◽  
Gergely Bernáth ◽  
Sławomir Krejszeff ◽  
János Radóczi ◽  
...  
Keyword(s):  
Long Term Storage ◽  
Short And Long Term ◽  
Term Storage

Effect of Short- and Long-Term Storage on Human Serum and Recombinant Apolipoprotein E Concentration

2000 ◽  
Vol 38 (6) ◽  
Author(s):  
Françoise Schiele ◽  
Monique Vincent-Viry ◽  
Bernard Herbeth ◽  
Athanase Visvikis ◽  
Gérard Siest
Keyword(s):  
Apolipoprotein E ◽  
Human Serum ◽  
Long Term Storage ◽  
Short And Long Term ◽  
Term Storage

Study of Short- and Long-Term Storage of Teeth and Its Influence on DNA

2009 ◽  
Vol 54 (6) ◽  
pp. 1411-1413 ◽  
Author(s):  
Leticia Rubio ◽  
Luis J. Martinez ◽  
Esther Martinez ◽  
Stella Martin de las Heras
Keyword(s):  
Long Term Storage ◽  
Short And Long Term ◽  
Term Storage

scholarly journals Preservation of Avian Cells at Sub-zero Temperatures

1979 ◽  
Vol 32 (5) ◽  
pp. 475 ◽  
Author(s):  
N Ratnamohan ◽  
PB Spradbrow
Keyword(s):  
Dimethyl Sulfoxide ◽  
Cryoprotective Agent ◽  
Cryoprotective Agents ◽  
Long Term Storage ◽  
Low Concentrations ◽  
Short And Long Term ◽  
Term Storage

The cryoprotective agents dimethyl sulfoxide (DMSO), glycerol, polyvinylpyrrolidone (PVP) and dextran were evaluated for their ability to protect avian cells during storage at sub-zero temperatures. DMSO was the most effective cryoprotective agent for the short- and long-term storage of avian cells and glycerol was also effective when used at low concentrations. PVP and dextran did not protect avian cells during storage in our experiments. Primary chicken cells and avian cells at higher passage levels were successfully recovered after storage with DMSO for periods ranging from 4 to 12 months.

scholarly journals Integrating Whole Blood Transcriptomic Collection Procedures Into the Current Anti-Doping Testing System, Including Long-Term Storage and Re-Testing of Anti-Doping Samples

2021 ◽  
Vol 8 ◽  
Author(s):  
Giscard Lima ◽  
Alexander Kolliari-Turner ◽  
Fernanda Rossell Malinsky ◽  
Fergus M. Guppy ◽  
Renan Paulo Martin ◽  
...  
Keyword(s):  
Whole Blood ◽  
Differentially Expressed ◽  
Rna Seq ◽  
Short Term ◽  
Long Term Storage ◽  
Short Term Storage ◽  
Short And Long Term ◽  
Rna Preservation ◽  
Term Storage

Introduction: Recombinant human erythropoietin (rHuEPO) administration studies involving transcriptomic approaches have demonstrated a gene expression signature that could aid blood doping detection. However, current anti-doping testing does not involve collecting whole blood into tubes with RNA preservative. This study investigated if whole blood in long-term storage and whole blood left over from standard hematological testing in short-term storage could be used for transcriptomic analysis despite lacking RNA preservation.Methods: Whole blood samples were collected from twelve and fourteen healthy nonathletic males, for long-term and short-term storage experiments. Long-term storage involved whole blood collected into Tempus™ tubes and K2EDTA tubes and subjected to long-term (i.e., ‒80°C) storage and RNA extracted. Short-term storage involved whole blood collected into K2EDTA tubes and stored at 4°C for 6‒48 h and then incubated at room temperature for 1 and 2 h prior to addition of RNA preservative. RNA quantity, purity, and integrity were analyzed in addition to RNA-Seq using the MGI DNBSEQ-G400 on RNA from both the short- and long-term storage studies. Genes presenting a fold change (FC) of >1.1 or < ‒1.1 with p ≤ 0.05 for each comparison were considered differentially expressed. Microarray analysis using the Affymetrix GeneChip® Human Transcriptome 2.0 Array was additionally conducted on RNA from the short-term study with a false discovery ratio (FDR) of ≤0.05 and an FC of >1.1 or < ‒1.1 applied to identify differentially expressed genes.Results: RNA quantity, purity, and integrity from whole blood subjected to short- and long-term storage were sufficient for gene expression analysis. Long-term storage: when comparing blood tubes with and without RNA preservation 4,058 transcripts (6% of coding and non-coding transcripts) were differentially expressed using microarray and 658 genes (3.4% of mapped genes) were differentially expressed using RNA-Seq. Short-term storage: mean RNA integrity and yield were not significantly different at any of the time points. RNA-Seq analysis revealed a very small number of differentially expressed genes (70 or 1.37% of mapped genes) when comparing samples stored between 6 and 48 h without RNA preservative. None of the genes previously identified in rHuEPO administration studies were differently expressed in either long- or short-term storage experiments.Conclusion: RNA quantity, purity, and integrity were not significantly compromised from short- or long-term storage in blood storage tubes lacking RNA stabilization, indicating that transcriptomic analysis could be conducted using anti-doping samples collected or biobanked without RNA preservation.

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