scholarly journals Preservation of Avian Cells at Sub-zero Temperatures

1979 ◽  
Vol 32 (5) ◽  
pp. 475 ◽  
Author(s):  
N Ratnamohan ◽  
PB Spradbrow
Keyword(s):  
Dimethyl Sulfoxide ◽  
Cryoprotective Agent ◽  
Cryoprotective Agents ◽  
Long Term Storage ◽  
Low Concentrations ◽  
Short And Long Term ◽  
Term Storage

The cryoprotective agents dimethyl sulfoxide (DMSO), glycerol, polyvinylpyrrolidone (PVP) and dextran were evaluated for their ability to protect avian cells during storage at sub-zero temperatures. DMSO was the most effective cryoprotective agent for the short- and long-term storage of avian cells and glycerol was also effective when used at low concentrations. PVP and dextran did not protect avian cells during storage in our experiments. Primary chicken cells and avian cells at higher passage levels were successfully recovered after storage with DMSO for periods ranging from 4 to 12 months.

scholarly journals Cryopreservation of Canine Erythrocytes Using Dimethyl Sulfoxide, Polyethylene Glycol and Sucrose

2021 ◽  
Vol 31 (1) ◽  
pp. 38-50
Author(s):  
Оlga Denysova ◽  
◽  
Gennadiy Zhegunov ◽  
Keyword(s):  
Polyethylene Glycol ◽  
Dimethyl Sulfoxide ◽  
Salt Concentration ◽  
Cryoprotective Agents ◽  
Long Term Storage ◽  
Osmotic Stability ◽  
Medium Increase ◽  
Term Storage ◽  
Saline Medium

Cryoprotective properties of combined media of permeable (dimethyl sulfoxide) and impermeable (polyethylene glycol with m. w. 1500) cryoprotective agents during rapid cooling in liquid nitrogen of canine erythrocytes using saline and sucrose-saline media have been investigated. It was found that the use of combined solutions of cryoprotective agents based on polyethylene glycol with m.w. 1500 (15%) and dimethyl sulfoxide (2.5–10%) in saline was not quite effective for cryopreservation of canine erythrocytes. Reducing the salt concentration and adding cell-impermeable sucrose to the cryopreservation medium increase the preservation of erythrocytes after warming. The best cryoprotective properties for canine erythrocytes were demonstrated by 10% dimethyl sulfoxide based on sucrose-saline medium, with high preservation of cells after freeze-warming, mechanical and osmotic stability of warmed erythrocytes. This indicates the possibility of a long-term storage and use of cryopreserved canine erythrocytes for transfusions.

scholarly journals Is adipose tissue suitable for detection of (synthetic) cannabinoids? A comparative study analyzing antemortem and postmortem specimens following pulmonary administration of JWH-210, RCS-4, as well as ∆9-tetrahydrocannabinol to pigs

2020 ◽  
Vol 94 (10) ◽  
pp. 3421-3431
Author(s):  
Nadine Schaefer ◽  
Frederike Nordmeier ◽  
Ann-Katrin Kröll ◽  
Christina Körbel ◽  
Matthias W. Laschke ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Synthetic Cannabinoids ◽  
Standard Addition ◽  
Storage Conditions ◽  
Long Term Storage ◽  
Low Concentrations ◽  
Concentration Changes ◽  
Term Storage ◽  
Plateau Level

Abstract Examining fatal poisonings, chronic exposure may be reflected by the concentration in tissues known for long-term storage of drugs. Δ9-tetrahydrocannabinol (THC) persists in adipose tissue (AT), but sparse data on synthetic cannabinoids (SC) are available. Thus, a controlled pig study evaluating antemortem (AM) disposition and postmortem (PM) concentration changes of the SC 4-ethylnaphthalene-1-yl-(1-pentylindole-3-yl)methanone (JWH-210) and 2-(4-methoxyphenyl)-1-(1-pentyl-indole-3-yl)methanone (RCS-4) as well as THC in AT was performed. The drugs were administered pulmonarily (200 µg/kg body weight) to twelve pigs. Subcutaneous (s.c.) AT specimens were collected after 15 and 30 min and then hourly up to 8 h. At the end, pigs were sacrificed and s.c., perirenal, and dorsal AT specimens were collected. The carcasses were stored at room temperature (RT; n = 6) or 4 °C (n = 6) and specimens were collected after 24, 48, and 72 h. After homogenization in acetonitrile and standard addition, LC–MS/MS was performed. Maximum concentrations were reached 0.5–2 h after administration amounting to 21 ± 13 ng/g (JWH-210), 24 ± 13 ng/g (RCS-4), and 22 ± 20 ng/g (THC) and stayed at a plateau level. Regarding the metabolites, very low concentrations of N-hydroxypentyl-RCS-4 (HO-RCS-4) were detected from 0.5 to 8 h. PM concentrations of parent compounds did not change significantly (p > 0.05) over time under both storage conditions. Concentrations of HO-RCS-4 significantly (p < 0.05) increased in perirenal AT during storage at RT. These results suggest a rapid distribution and persistence in s.c. AT. Furthermore, AT might be resistant to PM redistribution of parent compounds. However, significant PM increases of metabolite concentrations might be considered in perirenal AT.

Short- and Long-Term Storage of Gametes

2016 ◽  
pp. 53-60
Author(s):  
Daniel Żarski ◽  
Ákos Horváth ◽  
Gergely Bernáth ◽  
Sławomir Krejszeff ◽  
János Radóczi ◽  
...  
Keyword(s):  
Long Term Storage ◽  
Short And Long Term ◽  
Term Storage

Comparative analysis of the influence of cryoprotective agents and a long-term storage on human Adipose tissue-Mesenchimal Stromal Cells

Cytotherapy ◽  
2020 ◽  
Vol 22 (5) ◽  
pp. S100
Author(s):  
O. Espinosa Ibáñez ◽  
A. Fernández-González ◽  
Á. Sierra-Sánchez ◽  
J. Guerrero ◽  
N. Fernández-Porcel ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Comparative Analysis ◽  
Stromal Cells ◽  
Human Adipose Tissue ◽  
Cryoprotective Agents ◽  
Long Term Storage ◽  
Term Storage

Effect of Short- and Long-Term Storage on Human Serum and Recombinant Apolipoprotein E Concentration

2000 ◽  
Vol 38 (6) ◽  
Author(s):  
Françoise Schiele ◽  
Monique Vincent-Viry ◽  
Bernard Herbeth ◽  
Athanase Visvikis ◽  
Gérard Siest
Keyword(s):  
Apolipoprotein E ◽  
Human Serum ◽  
Long Term Storage ◽  
Short And Long Term ◽  
Term Storage

Study of Short- and Long-Term Storage of Teeth and Its Influence on DNA

2009 ◽  
Vol 54 (6) ◽  
pp. 1411-1413 ◽  
Author(s):  
Leticia Rubio ◽  
Luis J. Martinez ◽  
Esther Martinez ◽  
Stella Martin de las Heras
Keyword(s):  
Long Term Storage ◽  
Short And Long Term ◽  
Term Storage

Susceptibility of Japanese pears to low concentrations of ethylene during storage

2007 ◽  
Vol 47 (12) ◽  
pp. 1480 ◽  
Author(s):  
M. J. Szczerbanik ◽  
K. J. Scott ◽  
J. E. Paton ◽  
D. J. Best
Keyword(s):  
Carbon Dioxide ◽  
Low Cost ◽  
Pyrus Pyrifolia ◽  
Green Colour ◽  
Long Term Storage ◽  
Low Concentrations ◽  
Internal Browning ◽  
Term Storage

The ‘Nijisseiki’ cultivar of Japanese pears (Pyrus pyrifolia) is also known as nashi in Australia. Nashi were exposed to levels of <0.005, 0.01, 0.1 and 1.0 µL/L of ethylene in air during 26 weeks storage at 0°C. Levels of ethylene as low as 0.01 µL/L increased chlorophyll loss and visual green colour. Increasing ethylene levels also increased softening and internal browning, although flesh spot decay was reduced in the presence of ethylene. While it would be worthwhile to remove ethylene during long-term storage of ‘Nijisseiki’ in air, another alternative, adding 2% carbon dioxide to the atmosphere, is suggested as a possible low cost means to overcome the ripening effect of ethylene.

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