scholarly journals Extracellular matrix surface network is associated with non-morphogenic calli of Helianthus tuberosus cv. Albik produced from various explants

2014 ◽  
Vol 83 (1) ◽  
pp. 67-73 ◽  
Author(s):  
Maria Pilarska ◽  
Marzena Popielarska-Konieczna ◽  
Halina Ślesak ◽  
Małgorzata Kozieradzka-Kiszkurno ◽  
Grzegorz Góralski ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
In Vitro Regeneration ◽  
Vascular Bundles ◽  
Helianthus Tuberosus ◽  
Important Species ◽  
Electron Microscopies ◽  
Transmission Electron ◽  
Matrix Surface ◽  
Surface Network

<em>Helianthus tuberosus</em> is economically important species. To improve characters of this energetic plant via genetic modification, production of callus tissue and plant regeneration are the first steps. A new, potentially energetic cultivar Albik was used in this study to test callus induction and regeneration. Callus was produced on leaves, petioles, apical meristems and stems from field-harvested plants but was totally non-morphogenic. Its induction started in the cortex and vascular bundles as confirmed by histological analysis. The surface of heterogeneous callus was partially covered with a membranous extracellular matrix surface network visible in scanning and transmission electron microscopies. The results clearly indicate that: (<strong><em>i</em></strong>) the morphogenic capacity of callus in topinambur is genotype dependent, (<strong><em>ii</em></strong>) cv. Albik of <em>H. tuberosus</em> proved recalcitrant in in vitro regeneration, and (<strong><em>iii</em></strong>) extracellular matrix surface network is not a morphogenic marker in this cultivar.

scholarly journals Are extracellular matrix surface network components involved in signalling and protective function?

2008 ◽  
Vol 3 (9) ◽  
pp. 707-709 ◽  
Author(s):  
Marzena Popielarska-Konieczna ◽  
Małgorzata Kozieradzka-Kiszkurno ◽  
Joanna Świerczyńska ◽  
Grzegorz Góralski ◽  
Halina Ślesak ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Protective Function ◽  
Matrix Surface ◽  
Surface Network

scholarly journals Sensitivity of the Fasciae to the Endocannabinoid System: Production of Hyaluronan-Rich Vesicles and Potential Peripheral Effects of Cannabinoids in Fascial Tissue

2020 ◽  
Vol 21 (8) ◽  
pp. 2936 ◽  
Author(s):  
Caterina Fede ◽  
Carmelo Pirri ◽  
Lucia Petrelli ◽  
Diego Guidolin ◽  
Chenglei Fan ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cannabinoid Receptor ◽  
Endocannabinoid System ◽  
Small Samples ◽  
In Vitro Production ◽  
Cannabinoid Receptor 2 ◽  
Transmission Electron ◽  
Peripheral Effect ◽  
Vitro Production

The demonstrated expression of endocannabinoid receptors in myofascial tissue suggested the role of fascia as a source and modulator of pain. Fibroblasts can modulate the production of the various components of the extracellular matrix, according to type of stimuli: physical, mechanical, hormonal, and pharmacological. In this work, fascial fibroblasts were isolated from small samples of human fascia lata of the thigh, collected from three volunteer patients (two men, one woman) during orthopedic surgery. This text demonstrates for the first time that the agonist of cannabinoid receptor 2, HU-308, can lead to in vitro production of hyaluronan-rich vesicles only 3–4 h after treatment, being rapidly released into the extracellular environment. We demonstrated that these vesicles are rich in hyaluronan after Alcian blue and Toluidine blue stainings, immunocytochemistry, and transmission electron microscopy. In addition, incubation with the antagonist AM630 blocked vesicles production by cells, confirming that release of hyaluronan is a cannabinoid-mediated effect. These results may show how fascial cells respond to the endocannabinoid system by regulating and remodeling the formation of the extracellular matrix. This is a first step in our understanding of how therapeutic applications of cannabinoids to treat pain may also have a peripheral effect, altering the biosynthesis of the extracellular matrix in fasciae and, consequently, remodeling the tissue and its properties.

Ultrastructure and histochemical analysis of extracellular matrix surface network in kiwifruit endosperm-derived callus culture

2008 ◽  
Vol 27 (7) ◽  
pp. 1137-1145 ◽  
Author(s):  
M. Popielarska-Konieczna ◽  
M. Kozieradzka-Kiszkurno ◽  
J. Świerczyńska ◽  
G. Góralski ◽  
H. Ślesak ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Callus Culture ◽  
Histochemical Analysis ◽  
Matrix Surface ◽  
Surface Network

The acellular stroma of the chick cornea inhibits melanogenesis of the neural-crest-derived cells that colonize it.

Development ◽  
1985 ◽  
Vol 86 (1) ◽  
pp. 143-154
Author(s):  
Steven Campbell ◽  
Jonathan B. L. Bard
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Extracellular Matrix ◽  
Neural Crest ◽  
Corneal Stroma ◽  
Cell Types ◽  
Brown Pigment ◽  
Transmission Electron ◽  
Wide Range

Neural crest (NC) cells from the periorbital mesenchyme (POM) invade the acellular stroma of the chick cornea at stage 27 of development (∼6 days). The invading cells become collagenproducing fibroblasts while the NC cells remaining in the POM differentiate into a wide range of cell types, the most easily recognizable of which is the pigment-producing melanocyte. In this paper, we report observations on the differentiation in vitro of cells within and migrating from explants of corneal stroma and compare their behaviour with that of cells within and migrating from explants of the POM. In ∼70% of cases, POM explants produced black, eumelanin pigmentation within 2–3 days in culture and gave rise to a mixed outgrowth of fibroblasts and melanoblasts that produced brown pigment. In no case, however, did a corneal explant produce black pigment (so demonstrating that any POM contamination was negligible). However, in 28% of cultures from stage-27 and -28 corneas, some of the cells in the outgrowth contained brown pigment indistinguishable from that produced by the POM control, although the majority of the cells in each case were fibroblasts. Two lines of investigation demonstrated that this pigment was melanin: first, transmission electron microscopy showed that the pigment organelles were incompletely melanized, granular melanosomes; second, tests designed to demonstrate the presence of lipofuscin, an alternative pigment, proved negative. Migrating cells from older corneas, in contrast, showed no evidence of even the first stages of melanogenesis. These results show, first, that some of the NC cells that invade the cornea are at least bipotent and hence representative of the POM population rather than being a unique subgroup and, second, that the acellular stroma of the cornea determines the state of differentiation of the NC cells that colonize it. The results thus provide an unequivocal demonstration that extracellular matrix can induce postmigratory NC cells to differentiate into fibroblasts.

Extracellular Matrix Surface Network During Plant Regeneration in Wheat Anther Culture

2005 ◽  
Vol 83 (2) ◽  
pp. 201-208 ◽  
Author(s):  
R. Konieczny ◽  
J. Bohdanowicz ◽  
A.Z. Czaplicki ◽  
L. Przywara
Keyword(s):  
Extracellular Matrix ◽  
Plant Regeneration ◽  
Anther Culture ◽  
Matrix Surface ◽  
Surface Network

scholarly journals Influencing factors and structural characterization of hyperhydricity of in vitro regeneration in Brassica oleracea var. italica

2011 ◽  
Vol 91 (1) ◽  
pp. 159-165 ◽  
Author(s):  
Ya Yu ◽  
Yong-Qin Zhao ◽  
Bing Zhao ◽  
Shuxin Ren ◽  
Yang-Dong Guo
Keyword(s):  
Electron Microscopy ◽  
Brassica Oleracea ◽  
Influencing Factors ◽  
Structural Characterization ◽  
Indoleacetic Acid ◽  
Sucrose Concentration ◽  
In Vitro Regeneration ◽  
Transmission Electron

Yu, U., Zhao, Y.-Q., Zhao, B., Ren, S. and Guo, Y.-D. 2011. Influencing factors and structural characterization of hyperhydricity of in vitro regeneration in Brassica oleracea var. italica. Can. J. Plant Sci. 91: 159–165. This study examines factors that affect the occurrence of hyperhydric tissue in in vitro cultures of Brassica oleracea variety italica. The anatomy of normal and hyperhydric leaves of plantlets regenerated from the hypocotyls was compared using scanning electron microscopy and transmission electron microscopy. In hyperhydric leaves palisade tissue was absent and the spongy mesophyll displayed large, unorganized intercellular spaces. Hyperhydric leaves had abnormal stomata with deformed guard cells. Significant ultrastructural differences were observed between chloroplasts in normal and hyperhydric leaves. The effects of zeatin, indoleacetic acid, silver nitrate and sucrose on the formation of hyperhydric shoots were studied. Zeatin was the most important factor, followed by sucrose concentration, AgNO3 and indoleacetic acid. The process of hyperhydricity was found to be reversed by increasing the agar concentration and eliminating NH4NO3 from the macro-elements in the MS medium. This is the first report of hyperhydricity in Brassica oleracea, and our study gives a better understanding of the factors that influence hyperhydricity during in vitro regeneration in Brassica crops.

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