scholarly journals Floral biology of Senecio macrophyllus M. BIEB. (Asteraceae), a rare Central European steppe plant

2014 ◽  
Vol 83 (1) ◽  
pp. 17-27 ◽  
Author(s):  
Bożenna Czarnecka ◽  
Bożena Denisow
Keyword(s):  
Rare Species ◽  
Pollen Viability ◽  
In Situ Conservation ◽  
Pollen Grains ◽  
Main Function ◽  
Long Distance ◽  
Early Afternoon ◽  
Pollen Presentation ◽  
Insect Visitors

Knowledge of the flowering phase and plant pollination ecology is very important for understanding the life history of long-lived perennials. In the case of rare species, the information may have implications for conservation practices. Our studies on flower morphology and blooming biology of the vulnerable plant <em>Senecio macrophyllus</em> M. BIEB. were conducted in situ (flowering, activity of insect visitors) in the largest population in SE Poland and in laboratory (light and scanning electron microscopy). The disc florets open diurnally with most intensive anthesis in the early afternoon and attract insect visitors with nectar and pollen. In highly protandrous disc florets, pollen viability decreases in time, whereas stigma receptivity increases. The upper part of the pistil forms a brush-like pollen presenter, covered with unicellular trichomes with chromoplasts. Pollen presentation lasts 4–11 hours per floret, and 8 days in a single head, the main function of which is to extend the availability of male gamets for reproduction. The number of florets per head, the head size, and the number of pollen grains produced per anther, and the pollen grain viability differed significantly between microsites. The finding indicates that, apart from the biotic factors, abiotic conditions may considerably influence generative reproduction of the species. In the study area, the principal visitors of <em>S. macrophyllus</em> flowers were Hymenoptera, with predominance of <em>Apis mellifera</em> (53.4% of visits). The remarkable share of butterflies (13.9%) recognized as the long-distance moving insects may improve the genetic variability of individuals within a fragmented population. A more detailed study is necessary to explain the role of insect visitors in effective pollination and in gene transfer between patches. The assessment of optimal conditions for the generative reproduction is fundamental for the in situ conservation of this rare species.

Reproductive biology of Ophiopogon xylorrhizus (Liliaceae s.l.): an endangered endemic of Yunnan, Southwest China

2000 ◽  
Vol 48 (1) ◽  
pp. 101 ◽  
Author(s):  
Tian-hua He ◽  
Guang-yuan Rao ◽  
Rui-lin You
Keyword(s):  
Reproductive Biology ◽  
Southwest China ◽  
Airborne Pollen ◽  
In Situ Conservation ◽  
Pollen Grains ◽  
Single Species ◽  
Developmental Phase ◽  
Germinated Pollen ◽  
Mature Seeds

Reproductive biology of Ophiopogon xylorrhizus Wang et Tai was studied from 1995 to 1997 in Mengla County of Yunnan Province, Southwest China. The small, white or pale pink flowers with stigmas above anthers appeared from late March to early April in the study years, and produced abundant pollen, but no nectar. Pollen : ovule ratios were 17 185 ± 2175. Every flower opens only once and remains functional for 10–12 h. Pollen remains viable for the same period. During anthesis, flowers were visited only by single species of thrips (Taeniothrips sp., Thripidae). No airborne pollen grains were collected throughout the blooming season, indicating that flowers are not wind-pollinated. About 28% of stigmas of unopened flowers were found with germinated pollen grains and seed set took place in single flowers that were bagged at the bud stage, which indicated autogamy and autonomous self-pollination. Ovary walls ruptured 4–5 days after fertilisation, allowing seed production to occur. About 75% of ovules were fertilised and developed into young seeds, but only 12.5% developed into mature seeds in open pollination treatments. The remainder of ovules were aborted or destroyed by insects or animals during the long developmental phase from April to December. Individuals of O. xylorrhizus commonly produced a single inflorescence with 2–24 flowers (15.3 ± 6.5, n = 33), and 1–43 seeds (12.7 ± 6.7, n = 33) during the study period. Conservation management for this endangered species is urgently required and in situ conservation is probably the best method to conserve this species.

scholarly journals Secondary pollen presentation and foral traits of Heliconia psittacorum

2018 ◽  
Vol 24 (4) ◽  
pp. 451-458
Author(s):  
Talita Oliveira Nascimento ◽  
Patricia Campos Silva ◽  
Vivian Loges ◽  
Sandra Mariotto ◽  
Willian Krause ◽  
...  
Keyword(s):  
Pollen Tube ◽  
Temporal Dynamics ◽  
Pollen Viability ◽  
Pollen Grains ◽  
Pollen Deposition ◽  
Pollen Transfer ◽  
Stigma Receptivity ◽  
Secondary Pollen Presentation ◽  
Self Pollination ◽  
Pollen Presentation

The secondary presentation of pollen consists of a foral mechanism where the presentation of pollen occurs in other foral structures in addition to the anther, in order to increase the precision of the dispersion of the pollen by the vectors. This study aims to describe the temporal dynamics of secondary pollen presentation, and morphological and morphometric characteristics in stages of pre- anthesis and anthesis in genotypes of fve natural Heliconia psittacorum populations. For the study of foral morphometry traits of length of the fower, stamen, stigma and height of flament insertion in the petal in bud and fower were measured. The foral morphology of pre-anthesis buds and fowers in anthesis, the presence or absence of characteristics such as herkogamy, region of flament insertion in the petal, region of stylar hairs, and of secondary pollen deposition were evaluated. Treatments of controlled pollinations, self-pollination, geitonogamy, cross-pollination, natural pollination and growth to pollen tube were sampled. Floral herkogamy and pollen transfer to the adhered hairs in the stylar region were clearly observed during anthesis, constituting the frst record of occurrence of secondary pollen presentation in Heliconiaceae. Pollen tube growth was inhibited in the stigmatic, style and basal regions of the pistil. Natural fruiting produced little or no fruit. The positioning of the stamens above the stigma, pollen viability and stigma receptivity during anthesis of H. psittacorum fowers may favor self-pollination. The stylar hairs observed in all H. psittacorum populations’ help in the retention of pollen grains. The low fruiting rate in controlled and natural pollinations suggest that the main propagation form of H. psittacorum in the study areas is based on asexual reproduction.

THE ESSENTIAL CONTRIBUTION OF CAPTIVE SUMATRAN ELEPHANT IN ELEPHANT TRAINING CENTER, WAY KAMBAS NATIONAL PARK FOR WILDLIFE GENETICS CONSERVATION

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Elly Lestari Rustiati ◽  
Priyambodo Priyambodo ◽  
Yanti Yulianti ◽  
Eko Agus Srihanto ◽  
Dian Neli Pratiwi ◽  
...  
Keyword(s):  
National Park ◽  
In Situ Conservation ◽  
Training Center ◽  
Conservation Effort ◽  
In The Wild ◽  
Wildlife Genetics ◽  
Way Kambas National Park ◽  
Human Conflict ◽  
Essential Contribution

Way Kambas National Park (WKNP) is home of five protected big mammals including sumatran elephants.  It shares its border with 22 of 37 villages surrounding the national park.  Understanding their existence in the wild is a priority, and  wildlife genetics is a crucially needed. Besides poaching and habitat fragmentation, wildlife-human conflict is one big issue.  Elephant Training Center (ETC) in WKNP is built for semi in-situ conservation effort on captive sumatran elephants that mainly have conflict histories with local people.  Participative observation and bio-molecular analysis were conducted to learn the importance of captive Sumatran elephant for conservation effort.  Through captive sumatran elephants, database and applicable methods are expected to be developed supporting the conservation of their population in the wild.  Participative observation and molecular identification was carried on captive sumatran elephants in ETC, WKNP under multiple year Terapan grant of Ministry of Research and Technology Higher Education, Indonesia. Gene sequence and cytological analyses showed that the captive sumatran elephants are closely related and tend to be domesticated.  Translocation among ETC to avoid inbreeding, and maintaining the captive sumatran elephant as natural as possible are highly recommended. Developing genetic database can be a reference for both captive and wild sumatran elephants.

CONSERVATION OF RARE SPECIES PLANTS AND LICHENS IN SITU WITH PLANNED ANTHROPOGENIC ACTIVITIES: BASIC APPROACHES AND IMPLEMENTATION PRINCIPLES

2018 ◽  
Vol 12 (7-8) ◽  
pp. 38-45
Author(s):  
A. N. EFREMOV ◽  
N. V. PLIKINA ◽  
T. ABELI
Keyword(s):  
Rare Species ◽  
Technology Implementation ◽  
Technology Development ◽  
Anthropogenic Activities ◽  
Natural Habitat ◽  
Local Population ◽  
Main Stage ◽  
Social Risks

Rare species are most vulnerable to man-made impacts, due to their biological characteristics or natural resource management. As a rule, the economic impact is associated with the destruction and damage of individual organisms, the destruction or alienation of habitats. Unfortunately, the conservation of habitat integrity is an important protection strategy, which is not always achievable in the implementation of industrial and infrastructural projects. The aim of the publication is to summarize the experience in the field of protection of rare species in the natural habitat (in situ), to evaluate and analyze the possibility of using existing methods in design and survey activities. In this regard, the main methodological approaches to the protection of rare species in the natural habitat (in situ) during the proposed economic activity were reflected. The algorithm suggested by the authors for implementing the in situ project should include a preparatory stage (initial data collection, preliminary risk assessments, technology development, obtaining permitting documentation), the main stage, the content of which is determined by the selected technology and a long monitoring stage, which makes it possible to assess the effectiveness of the taken measures. Among the main risks of in situ technology implementation, the following can be noted: the limited resources of the population that do not allow for the implementation of the procedure without prior reproduction of individuals in situ (in vitro); limited knowledge of the biology of the species; the possibility of invasion; the possibility of crossing for closely related species that сo-exist in the same habitat; social risks and consequences, target species or population may be important for the local population; financial risks during the recovery of the population. The available experience makes it possible to consider the approach to the conservation of rare species in situ as the best available technology that contributes to reducing negative environmental risks.

Status Analysis of Linpan Protection in Land Consolidation of Chengdu Plain

2013 ◽  
Vol 357-360 ◽  
pp. 2118-2121
Author(s):  
Ling Li Jia ◽  
Heng Cui
Keyword(s):  
In Situ Conservation ◽  
Land Consolidation ◽  
Architectural Style ◽  
Evaluation Standard ◽  
The Public ◽  
Chengdu Plain ◽  
Settlement Type ◽  
Comprehensive Development ◽  
Status Analysis

In the process of land consolidation in Chengdu Plain, Linpan protection is an important content. At present, some protection types of Linpan have been formed in Chengdu area, such as agriculture, rural tourism, special industry and settlement type and so on. Many protected modes were explored, such as the natural subsidies, in situ conservation, comprehensive development, off-site reconstruction, etc. But there are still some questions, do not pay attention to protect Linpan ecological pattern plate function transformation, the architectural style of hybrid, protection methods are not flexible, evaluation standard is not perfect, the public participation is not enough and other issues, these problems need to be continuously optimized and improved in the future.

scholarly journals A Duplicated Copy of the Meiotic Gene ZIP4 Preserves up to 50% Pollen Viability and Grain Number in Polyploid Wheat

Biology ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 290
Author(s):  
Abdul Kader Alabdullah ◽  
Graham Moore ◽  
Azahara C. Martín
Keyword(s):  
Pollen Viability ◽  
Pollen Grains ◽  
Wheat Chromosome ◽  
Grain Number ◽  
Meiotic Gene ◽  
Chromosome 5B ◽  
Mutant Pollen ◽  
Female Gametogenesis ◽  
Chromosome 3B

Although most flowering plants are polyploid, little is known of how the meiotic process evolves after polyploidisation to stabilise and preserve fertility. On wheat polyploidisation, the major meiotic gene ZIP4 on chromosome 3B duplicated onto 5B and diverged (TaZIP4-B2). TaZIP4-B2 was recently shown to promote homologous pairing, synapsis and crossover, and suppress homoeologous crossover. We therefore suspected that these meiotic stabilising effects could be important for preserving wheat fertility. A CRISPR Tazip4-B2 mutant was exploited to assess the contribution of the 5B duplicated ZIP4 copy in maintaining pollen viability and grain setting. Analysis demonstrated abnormalities in 56% of meiocytes in the Tazip4-B2 mutant, with micronuclei in 50% of tetrads, reduced size in 48% of pollen grains and a near 50% reduction in grain number. Further studies showed that most of the reduced grain number occurred when Tazip4-B2 mutant plants were pollinated with the less viable Tazip4-B2 mutant pollen rather than with wild type pollen, suggesting that the stabilising effect of TaZIP4-B2 on meiosis has a greater consequence in subsequent male, rather than female gametogenesis. These studies reveal the extraordinary value of the wheat chromosome 5B TaZIP4-B2 duplication to agriculture and human nutrition. Future studies should further investigate the role of TaZIP4-B2 on female fertility and assess whether different TaZIP4-B2 alleles exhibit variable effects on meiotic stabilisation and/or resistance to temperature change.

scholarly journals Status of the Ex Situ and In Situ Conservation of Brazilian Crop Wild Relatives of Rice, Potato, Sweet Potato, and Finger Millet: Filling the Gaps of Germplasm Collections

Agronomy ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 638
Author(s):  
Marcelo B. Medeiros ◽  
José F. M. Valls ◽  
Aluana G. Abreu ◽  
Gustavo Heiden ◽  
Suelma Ribeiro-Silva ◽  
...  
Keyword(s):  
Finger Millet ◽  
Ex Situ Conservation ◽  
Gap Analysis ◽  
In Situ Conservation ◽  
Germplasm Collection ◽  
Crop Wild Relatives ◽  
Wild Relatives ◽  
Ex Situ ◽  
The Status

This study presents the status of ex situ and in situ conservation for the crop wild relatives of rice, potato, sweet potato, and finger millet in Brazil, and the subsequent germplasm collection expeditions. This research is part of a global initiative entitled “Adapting Agriculture to Climate Change: Collecting, Protecting, and Preparing Crop Wild Relatives” supported by the Global Crop Diversity Trust. Species of the primary, secondary, and tertiary gene pools with occurrences reported in Brazil were included: Oryza alta Swallen, O. grandiglumis (Döll) Prod., O. latifolia Desv., O. glumaepatula Steud., Eleusine tristachya (Lam.) Lam., E. indica (L.) Gaertn., Solanum commersonii Dunal, S. chacoense Bitter, Ipomoea grandifolia (Dammer) O’Donell, I. ramosissima (Poir.) Choisy, I. tiliacea (Willd.) Choisy, I. triloba L., and I. cynanchifolia Meisn. The status of the ex situ and in situ conservation of each taxon was assessed using the gap analysis methodology, and the results were used to plan 16 germplasm collection expeditions. Seeds of the collected material were evaluated for viability, and the protocols for seed germination and cryopreservation were tested. The final conservation score, resulting from the gap analysis and including the average of the ex situ and in situ scores, resulted in a classification of medium priority of conservation for all the species, with the exception of I. grandifolia (high priority). The total accessions collected (174) almost doubled the total accessions of these crop wild relatives incorporated in Embrapa’s ex situ conservation system prior to 2015. In addition, accessions for practically absent species were collected for the ex situ conservation system, such as Ipomoea species, Eleusine indica, and Solanum chacoense. The methods used for dormancy breaking and low temperature conservation for the Oryza, Eleusine, and Ipomoea species were promising for the incorporation of accessions in the respective gene banks. The results show the importance of efforts to collect and conserve ex situ crop wild relatives in Brazil based on previous gap analysis. The complementarity with the in situ strategy also appears to be very promising in the country.

scholarly journals Chromosomal painting of the sandpiper (Actitis macularius) detects several fissions for the Scolopacidae family (Charadriiformes)

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Melquizedec Luiz Silva Pinheiro ◽  
Cleusa Yoshiko Nagamachi ◽  
Talita Fernanda Augusto Ribas ◽  
Cristovam Guerreiro Diniz ◽  
Patricia Caroline Mary O´Brien ◽  
...  
Keyword(s):  
Chromosome Painting ◽  
Diploid Number ◽  
Chromosomal Evolution ◽  
Long Distance ◽  
Chromosomal Painting ◽  
Cytogenetic Studies ◽  
Burhinus Oedicnemus ◽  
Clear Idea ◽  
First Time

Abstract Background The Scolopacidae family (Suborder Scolopaci, Charadriiformes) is composed of sandpipers and snipes; these birds are long-distance migrants that show great diversity in their behavior and habitat use. Cytogenetic studies in the Scolopacidae family show the highest diploid numbers for order Charadriiformes. This work analyzes for the first time the karyotype of Actitis macularius by classic cytogenetics and chromosome painting. Results The species has a diploid number of 92, composed mostly of telocentric pairs. This high 2n is greater than the proposed 80 for the avian ancestral putative karyotype (a common feature among Scolopaci), suggesting that fission rearrangements have formed smaller macrochromosomes and microchromosomes. Fluorescence in situ hybridization using Burhinus oedicnemus whole chromosome probes confirmed the fissions in pairs 1, 2, 3, 4 and 6 of macrochromosomes. Conclusion Comparative analysis with other species of Charadriiformes studied by chromosome painting together with the molecular phylogenies for the order allowed us to raise hypotheses about the chromosomal evolution in suborder Scolopaci. From this, we can establish a clear idea of how chromosomal evolution occurred in this suborder.

scholarly journals Gap analyses of priority wild relatives of food crop in current ex situ and in situ conservation in Indonesia

2021 ◽  
Author(s):  
Wiguna Rahman ◽  
Joana Magos Brehm ◽  
Nigel Maxted ◽  
Jade Phillips ◽  
Aremi R. Contreras-Toledo ◽  
...  
Keyword(s):  
Protected Areas ◽  
Protected Area ◽  
In Situ Conservation ◽  
Wild Relatives ◽  
Ex Situ ◽  
Area Network ◽  
Complementarity Analysis ◽  
Ex Situ Collections ◽  
Conservation Actions

AbstractConservation programmes are always limited by available resources. Careful planning is therefore required to increase the efficiency of conservation and gap analysis can be used for this purpose. This method was used to assess the representativeness of current ex situ and in situ conservation actions of 234 priority crop wild relatives (CWR) in Indonesia. This analysis also included species distribution modelling, the creation of an ecogeographical land characterization map, and a complementarity analysis to identify priorities area for in situ conservation and for further collecting of ex situ conservation programmes. The results show that both current ex situ and in situ conservation actions are insufficient. Sixty-six percent of priority CWRs have no recorded ex situ collections. Eighty CWRs with ex situ collections are still under-represented in the national genebanks and 65 CWRs have no presence records within the existing protected area network although 60 are predicted to exist in several protected areas according to their potential distribution models. The complementarity analysis shows that a minimum of 61 complementary grid areas (complementary based on grid cells) are required to conserve all priority taxa and 40 complementary protected areas (complementary based on existing protected areas) are required to conserve those with known populations within the existing in situ protected area network. The top ten of complementary protected areas are proposed as the initial areas for the development of CWR genetic reserves network in Indonesia. It is recommended to enhanced coordination between ex situ and in situ conservation stakeholders for sustaining the long term conservation of CWR in Indonesia. Implementation of the research recommendations will provide for the first time an effective conservation planning of Indonesia’s CWR diversity and will significantly enhance the country’s food and nutritional security.

Correlation of Grassy Lake and Cedar Lake ambers using infrared spectroscopy, stable isotopes, and palaeoentomology

2008 ◽  
Vol 45 (9) ◽  
pp. 1061-1082 ◽  
Author(s):  
Ryan C. McKellar ◽  
Alexander P. Wolfe ◽  
Ralf Tappert ◽  
Karlis Muehlenbachs
Keyword(s):  
Stable Isotopes ◽  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Late Cretaceous ◽  
Western Canada ◽  
Long Distance ◽  
Geochemical Fingerprinting ◽  
Long Distance Transport ◽  
Distance Transport

The Late Cretaceous Grassy Lake and Cedar Lake amber deposits of western Canada are among North America’s most famous amber-producing localities. Although it has been suggested for over a century that Cedar Lake amber from western Manitoba may be a secondary deposit having originated from strata in Alberta, this hypothesis has not been tested explicitly using geochemical fingerprinting coupled to comparative analyses of arthropod faunal content. Although there are many amber-containing horizons associated with Cretaceous coals throughout Alberta, most are thermally mature and brittle, thus lacking the resilience to survive long distance transport while preserving intact biotic inclusions. One of the few exceptions is the amber found in situ at Grassy Lake. We present a suite of new analyses from these and other Late Cretaceous ambers from western Canada, including stable isotopes (H and C), Fourier transform infrared (FTIR) spectra, and an updated faunal compendium for the Grassy and Cedar lakes arthropod assemblages. When combined with amber’s physical properties and stratigraphic constraints, the results of these analyses confirm that Cedar Lake amber is derived directly from the Grassy Lake amber deposit or an immediate correlative equivalent. This enables the palaeoenvironmental context of Grassy Lake amber to be extended to the Cedar Lake deposit, making possible a more inclusive survey of Cretaceous arthropod faunas.

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