Reproductive biology of Ophiopogon xylorrhizus (Liliaceae s.l.): an endangered endemic of Yunnan, Southwest China

2000 ◽  
Vol 48 (1) ◽  
pp. 101 ◽  
Author(s):  
Tian-hua He ◽  
Guang-yuan Rao ◽  
Rui-lin You

Reproductive biology of Ophiopogon xylorrhizus Wang et Tai was studied from 1995 to 1997 in Mengla County of Yunnan Province, Southwest China. The small, white or pale pink flowers with stigmas above anthers appeared from late March to early April in the study years, and produced abundant pollen, but no nectar. Pollen : ovule ratios were 17 185 ± 2175. Every flower opens only once and remains functional for 10–12 h. Pollen remains viable for the same period. During anthesis, flowers were visited only by single species of thrips (Taeniothrips sp., Thripidae). No airborne pollen grains were collected throughout the blooming season, indicating that flowers are not wind-pollinated. About 28% of stigmas of unopened flowers were found with germinated pollen grains and seed set took place in single flowers that were bagged at the bud stage, which indicated autogamy and autonomous self-pollination. Ovary walls ruptured 4–5 days after fertilisation, allowing seed production to occur. About 75% of ovules were fertilised and developed into young seeds, but only 12.5% developed into mature seeds in open pollination treatments. The remainder of ovules were aborted or destroyed by insects or animals during the long developmental phase from April to December. Individuals of O. xylorrhizus commonly produced a single inflorescence with 2–24 flowers (15.3 ± 6.5, n = 33), and 1–43 seeds (12.7 ± 6.7, n = 33) during the study period. Conservation management for this endangered species is urgently required and in situ conservation is probably the best method to conserve this species.

2014 ◽  
Vol 83 (1) ◽  
pp. 17-27 ◽  
Author(s):  
Bożenna Czarnecka ◽  
Bożena Denisow

Knowledge of the flowering phase and plant pollination ecology is very important for understanding the life history of long-lived perennials. In the case of rare species, the information may have implications for conservation practices. Our studies on flower morphology and blooming biology of the vulnerable plant <em>Senecio macrophyllus</em> M. BIEB. were conducted in situ (flowering, activity of insect visitors) in the largest population in SE Poland and in laboratory (light and scanning electron microscopy). The disc florets open diurnally with most intensive anthesis in the early afternoon and attract insect visitors with nectar and pollen. In highly protandrous disc florets, pollen viability decreases in time, whereas stigma receptivity increases. The upper part of the pistil forms a brush-like pollen presenter, covered with unicellular trichomes with chromoplasts. Pollen presentation lasts 4–11 hours per floret, and 8 days in a single head, the main function of which is to extend the availability of male gamets for reproduction. The number of florets per head, the head size, and the number of pollen grains produced per anther, and the pollen grain viability differed significantly between microsites. The finding indicates that, apart from the biotic factors, abiotic conditions may considerably influence generative reproduction of the species. In the study area, the principal visitors of <em>S. macrophyllus</em> flowers were Hymenoptera, with predominance of <em>Apis mellifera</em> (53.4% of visits). The remarkable share of butterflies (13.9%) recognized as the long-distance moving insects may improve the genetic variability of individuals within a fragmented population. A more detailed study is necessary to explain the role of insect visitors in effective pollination and in gene transfer between patches. The assessment of optimal conditions for the generative reproduction is fundamental for the in situ conservation of this rare species.


2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Elly Lestari Rustiati ◽  
Priyambodo Priyambodo ◽  
Yanti Yulianti ◽  
Eko Agus Srihanto ◽  
Dian Neli Pratiwi ◽  
...  

Way Kambas National Park (WKNP) is home of five protected big mammals including sumatran elephants.  It shares its border with 22 of 37 villages surrounding the national park.  Understanding their existence in the wild is a priority, and  wildlife genetics is a crucially needed. Besides poaching and habitat fragmentation, wildlife-human conflict is one big issue.  Elephant Training Center (ETC) in WKNP is built for semi in-situ conservation effort on captive sumatran elephants that mainly have conflict histories with local people.  Participative observation and bio-molecular analysis were conducted to learn the importance of captive Sumatran elephant for conservation effort.  Through captive sumatran elephants, database and applicable methods are expected to be developed supporting the conservation of their population in the wild.  Participative observation and molecular identification was carried on captive sumatran elephants in ETC, WKNP under multiple year Terapan grant of Ministry of Research and Technology Higher Education, Indonesia. Gene sequence and cytological analyses showed that the captive sumatran elephants are closely related and tend to be domesticated.  Translocation among ETC to avoid inbreeding, and maintaining the captive sumatran elephant as natural as possible are highly recommended. Developing genetic database can be a reference for both captive and wild sumatran elephants.


2013 ◽  
Vol 357-360 ◽  
pp. 2118-2121
Author(s):  
Ling Li Jia ◽  
Heng Cui

In the process of land consolidation in Chengdu Plain, Linpan protection is an important content. At present, some protection types of Linpan have been formed in Chengdu area, such as agriculture, rural tourism, special industry and settlement type and so on. Many protected modes were explored, such as the natural subsidies, in situ conservation, comprehensive development, off-site reconstruction, etc. But there are still some questions, do not pay attention to protect Linpan ecological pattern plate function transformation, the architectural style of hybrid, protection methods are not flexible, evaluation standard is not perfect, the public participation is not enough and other issues, these problems need to be continuously optimized and improved in the future.


Agronomy ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 638
Author(s):  
Marcelo B. Medeiros ◽  
José F. M. Valls ◽  
Aluana G. Abreu ◽  
Gustavo Heiden ◽  
Suelma Ribeiro-Silva ◽  
...  

This study presents the status of ex situ and in situ conservation for the crop wild relatives of rice, potato, sweet potato, and finger millet in Brazil, and the subsequent germplasm collection expeditions. This research is part of a global initiative entitled “Adapting Agriculture to Climate Change: Collecting, Protecting, and Preparing Crop Wild Relatives” supported by the Global Crop Diversity Trust. Species of the primary, secondary, and tertiary gene pools with occurrences reported in Brazil were included: Oryza alta Swallen, O. grandiglumis (Döll) Prod., O. latifolia Desv., O. glumaepatula Steud., Eleusine tristachya (Lam.) Lam., E. indica (L.) Gaertn., Solanum commersonii Dunal, S. chacoense Bitter, Ipomoea grandifolia (Dammer) O’Donell, I. ramosissima (Poir.) Choisy, I. tiliacea (Willd.) Choisy, I. triloba L., and I. cynanchifolia Meisn. The status of the ex situ and in situ conservation of each taxon was assessed using the gap analysis methodology, and the results were used to plan 16 germplasm collection expeditions. Seeds of the collected material were evaluated for viability, and the protocols for seed germination and cryopreservation were tested. The final conservation score, resulting from the gap analysis and including the average of the ex situ and in situ scores, resulted in a classification of medium priority of conservation for all the species, with the exception of I. grandifolia (high priority). The total accessions collected (174) almost doubled the total accessions of these crop wild relatives incorporated in Embrapa’s ex situ conservation system prior to 2015. In addition, accessions for practically absent species were collected for the ex situ conservation system, such as Ipomoea species, Eleusine indica, and Solanum chacoense. The methods used for dormancy breaking and low temperature conservation for the Oryza, Eleusine, and Ipomoea species were promising for the incorporation of accessions in the respective gene banks. The results show the importance of efforts to collect and conserve ex situ crop wild relatives in Brazil based on previous gap analysis. The complementarity with the in situ strategy also appears to be very promising in the country.


Author(s):  
Wiguna Rahman ◽  
Joana Magos Brehm ◽  
Nigel Maxted ◽  
Jade Phillips ◽  
Aremi R. Contreras-Toledo ◽  
...  

AbstractConservation programmes are always limited by available resources. Careful planning is therefore required to increase the efficiency of conservation and gap analysis can be used for this purpose. This method was used to assess the representativeness of current ex situ and in situ conservation actions of 234 priority crop wild relatives (CWR) in Indonesia. This analysis also included species distribution modelling, the creation of an ecogeographical land characterization map, and a complementarity analysis to identify priorities area for in situ conservation and for further collecting of ex situ conservation programmes. The results show that both current ex situ and in situ conservation actions are insufficient. Sixty-six percent of priority CWRs have no recorded ex situ collections. Eighty CWRs with ex situ collections are still under-represented in the national genebanks and 65 CWRs have no presence records within the existing protected area network although 60 are predicted to exist in several protected areas according to their potential distribution models. The complementarity analysis shows that a minimum of 61 complementary grid areas (complementary based on grid cells) are required to conserve all priority taxa and 40 complementary protected areas (complementary based on existing protected areas) are required to conserve those with known populations within the existing in situ protected area network. The top ten of complementary protected areas are proposed as the initial areas for the development of CWR genetic reserves network in Indonesia. It is recommended to enhanced coordination between ex situ and in situ conservation stakeholders for sustaining the long term conservation of CWR in Indonesia. Implementation of the research recommendations will provide for the first time an effective conservation planning of Indonesia’s CWR diversity and will significantly enhance the country’s food and nutritional security.


Toxins ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 438
Author(s):  
Mary E. Ridout ◽  
Bruce Godfrey ◽  
George Newcombe

Fusarium species coexist as toxigenic, systemic pathogens in sweet corn seed production in southwestern Idaho, USA. We hypothesized that fungal antagonists of seedborne Fusarium would differentially alter production of Fusarium mycotoxins directly and/or systemically. We challenged the Fusarium complex by in vitro antagonism trials and in situ silk and seed inoculations with fungal antagonists. Fungal antagonists reduced growth and sporulation of Fusarium species in vitro from 40.5% to as much as 100%. Pichia membranifaciens and Penicillium griseolum reduced fumonisin production by F. verticillioides by 73% and 49%, respectively, while P. membranifaciens and a novel Penicillium sp. (WPT) reduced fumonisins by F. proliferatum 56% and 78%, respectively. In situ, pre-planting inoculation of seeds with Penicillium WPT systemically increased fumonisins in the resulting crop. Morchella snyderi applied to silks of an F1 cross systemically reduced deoxynivalenol by 47% in mature seeds of the F2. Antagonists failed to suppress Fusarium in mature kernels following silk inoculations, although the ratio of F. verticillioides to total Fusarium double with some inoculants. Fusarium mycotoxin concentrations in sweet corn seed change systemically, as well as locally, in response to the presence of fungal antagonists, although in Fusarium presence in situ was not changed.


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