scholarly journals Physiological-biochemical parameters and characteristics of seed coat structure in lupin seeds subjected to long storage at different temperatures

2011 ◽  
Vol 77 (3) ◽  
pp. 201-205 ◽  
Author(s):  
Agnieszka I. Piotrowicz-Cieślak ◽  
Dariusz J. Michalczyk ◽  
Kamila Górska ◽  
Zofia Bulińska-Radomska ◽  
Ryszard J. Górecki
Keyword(s):  
Seed Coat ◽  
Room Temperature ◽  
Seed Storage ◽  
Lupinus Luteus ◽  
Soluble Carbohydrates ◽  
Raffinose Family Oligosaccharides ◽  
Seed Vigour ◽  
Long Term Storage ◽  
Different Temperatures ◽  
Lupin Seeds

Seed vigour, viability, the contents of soluble carbohydrates, total protein, albumins, and globulins, as well as seed coat structure, were analysed in yellow lupin (<em>Lupinus luteus</em> L.) cv. Iryd seeds stored for 20 years at -14<sup>o</sup>C, 0<sup>o</sup>C or at room temperature (approx. +20<sup>o</sup>C). Seed storage at room temperature reduced viability (to 2%) and increased seed leachate electroconductivity. Determinations of total proteins showed that protein content was significantly reduced in seeds stored at +20<sup>o</sup>C compared to the other storage regimens. Raffinose family oligosaccharides were the main soluble carbohydrates in seeds stored at 0<sup>o</sup>C and -14<sup>o</sup>C, whereas sucrose dominated in seeds stored at room temperature. Scanning electron microscopy (SEM) of seed surface and seed coat sections revealed appearance of an amorphic layer on the surface of seeds stored at room temperature (not observed in other seeds) and distinct shrinking of macrosclereid layer in seeds stored at -14<sup>o</sup>C. Macrosclereids layer in all seeds was 100 um thick and accounted for 60% of seed coat thickness. The obtained results suggest that for long term storage of lupin seeds at 0<sup>o</sup>C is the most advisable temperature if both costs of storage and seed storability are considered.

scholarly journals Studies on soluble carbohydrates in yellow lupin (Lupinus luteus L.) seeds of different age

2011 ◽  
Vol 76 (4) ◽  
pp. 309-312 ◽  
Author(s):  
Kazimierz Zalewski ◽  
Lesław B. Lahuta
Keyword(s):  
Seed Storage ◽  
Dry Mass ◽  
Lupinus Luteus ◽  
Soluble Carbohydrates ◽  
Mass Ratio ◽  
Yellow Lupin ◽  
Embryonic Axes ◽  
Laboratory Conditions ◽  
Lupin Seeds

Yellow lupin seeds cv. Juno were stored under laboratory conditions for 2 month, 4, 6 and 8 years. Eighteen soluble carbohydrates were identified in embryonic axes and cotyledons of different age seeds. The concentration of soluble carbohydrates in analysed seeds ranged from 25 to 34% of dry mass. Axes contained more carbohydrates than cotyledons. Stachyose dominated in axes, and verbascose - in cotyledons. Other detected galactosides were: galactinol, galactosyl pinitols and galactosyl chiro-inositols (fagopyritols), but their content was several-fold lower than that of RFOs (in both axes and cotyledons tissues). The concentration of soluble carbohydrates indicated, that sucrose to RFOs mass ratio, or other changes in sugars composition are not indicators of seed storage.

Soluble carbohydrates in desiccation tolerance of yellow lupin seeds during maturation and germination

1997 ◽  
Vol 7 (2) ◽  
pp. 107-116 ◽  
Author(s):  
R. J. Górecki ◽  
A. I. Piotrowicz-Cieślak ◽  
L. B. Lahuta ◽  
R. L. Obendorf
Keyword(s):  
Desiccation Tolerance ◽  
Tolerance Test ◽  
Soluble Carbohydrates ◽  
Raffinose Family Oligosaccharides ◽  
Yellow Lupin ◽  
Seed Growth ◽  
Before And After ◽  
Lupin Seeds ◽  
Root Tissues ◽  
Galactosyl Cyclitols

AbstractMaturing yellow lupin seeds were desiccation tolerant. Glucose, sucrose and cyclitols (mainly D-pinitol, D-chiro-inositol and myo-inositol) were predominant at the early stages of seed growth. Accumulation of the raffinose family oligosaccharides (RFOs) and the galactosyl cyclitols including galactinol, digalactosyl myo-inositol, galactopinitol A, galactopinitol B, trigalactopinitol A, ciceritol, fagopyritol B1 and fagopyritol B2 appeared during seed maturation; their increase correlated with seed germinability after desiccation. The loss of desiccation tolerance after seed germination was also studied. For the desiccation tolerance test, intact seedlings were dried rapidly or slowly followed by rehydration. Soluble carbohydrates were assayed before and after drying. Root tissues were more sensitive to desiccation than hypocotyl tissues and completely lost desiccation tolerance within 36 h of imbibition after both fast and slow-drying treatments. Survival of hypocotyls decreased gradually up to 96 h after imbibition. Loss of RFOs and galactosyl cyclitols in axis tissues preceded visible germination. Loss of desiccation tolerance was accompanied by loss of RFOs and galactosyl cyclitols and an increase in reducing sugars in cotyledon, hypocotyl and radicle tissues. Drying did not induce the accumulation of RFOs and galactosyl cyclitols in seedling tissues.

Changes in endogenous hormones and seed-coat phenolics during seed storage of two Suaeda salsa populations

2016 ◽  
Vol 64 (4) ◽  
pp. 325 ◽  
Author(s):  
Yan-ge Xu ◽  
Ranran Liu ◽  
Na Sui ◽  
Weiwei Shi ◽  
Lei Wang ◽  
...  
Keyword(s):  
Seed Coat ◽  
Seed Storage ◽  
Suaeda Salsa ◽  
Endogenous Hormones ◽  
Seed Vigour ◽  
Opposite Trend ◽  
Population Concentration ◽  
Intertidal Population ◽  
Black Seeds ◽  
And Storage

Changes in endogenous hormones and seed-coat phenolics during the storage of the dimorphic seeds of two Suaeda salsa populations were investigated. The results showed that, regardless of salinity, storage did not reduce the germination of brown or black seeds from an inland population, but it significantly decreased the germination of dimorphic seeds from an intertidal population. Abscisic acid (ABA) concentration was significantly higher in old seeds than in fresh seeds (freshly harvested dry seeds), whereas the opposite trend was evident for gibberellic acid (GA, including GA1 and GA3) concentration and GA : ABA ratio in both populations, and especially in the intertidal population. Concentration of seed-coat phenolics extracted from brown seeds was much higher than in black seeds in both populations, regardless of extracting time and storage. Storage increased the concentration of phenolics extracted from brown seeds in the inland population, whereas the opposite trend was evident in the intertidal population. Storage did not significantly change seed-coat phenolics extracted from black seeds relative to brown seeds in either population. The results indicated that changes in seed vigour during storage in dimorphic seeds of the two S. salsa populations are related to changes in endogenous hormones and seed-coat phenolics.

scholarly journals Kinetics of solute leachate from imbibing Caesalpinia echinata Lam. (Brazilwood) seeds

Revista CERES ◽  
2014 ◽  
Vol 61 (1) ◽  
pp. 90-97 ◽  
Author(s):  
Nestor Martini Neto ◽  
Edmir Vicente Lamarca ◽  
Claudio José Barbedo
Keyword(s):  
Seed Coat ◽  
Room Temperature ◽  
Initial Moisture Content ◽  
Leaching Kinetics ◽  
Leaching Rate ◽  
Different Temperatures ◽  
Physiological Quality ◽  
Rapid Tests ◽  
Caesalpinia Echinata ◽  
Kinetics Of

The electrical conductivity of leachates from imbibing seeds has been used as a vigor test for several species. The adaptation of this methodology to different species requires knowledge on the leaching kinetics of electrolytes. For Brazilwood seeds, the classic method was not satisfactory and rapid tests are essential because they have low storage capacity at room temperature. Leaching kinetics during seed imbibition is a function of physiological quality, presence or absence of seed coat, imbibing temperature and the initial moisture content of seed. In this study, the electrolyte leaching rate of six different categories of seeds, from two regions, was evaluated in seeds with and without seed coat and incubated with different moisture contents and at different temperatures. The results showed that the electrolyte leaching rate in Brazilwood seeds is independent of the physiological quality, the presence or absence of seed coat and imbibition temperature, but these factors changed the total amount of electrolytes leached. The leaching rate increased in the first few minutes of imbibition, suggesting that the adjustment of the methodology must consider the reduction in imbibition time, reduction in temperature, use of a controlled and slower pre-imbibition, and replacement of the imbibition solution after the first few minutes.

EFFECTS OF STORAGE CONDITIONS AND PRESENCE OF FRUITING BRACTS ON THE GERMINATION OF ATRIPLEX HALIMUS AND SALSOLA VERMICULATA

1997 ◽  
Vol 33 (2) ◽  
pp. 149-155 ◽  
Author(s):  
A. E. OSMAN ◽  
F. GHASSALI
Keyword(s):  
Seed Germination ◽  
Cold Storage ◽  
North Africa ◽  
Room Temperature ◽  
Storage Temperature ◽  
Seed Storage ◽  
Storage Conditions ◽  
Shrub Species ◽  
Atriplex Halimus ◽  
Different Temperatures

Two shrub species, Atriplex halimus L. and Salsola vermiculata L., are considered useful for rehabilitation of degraded rangelands in west Asia and north Africa. They can be established from direct seeding and are capable of self-sowing. In this study, seed storage at different temperatures and the influence of fruiting bracts on seed germination were examined for the two species during two seasons. Fruits (utricles) were stored at 20–22°C (room temperature), 0°C or −22°C. Germination tests were carried out after 33, 56, 90, 152, 272 and 397 d in storage in the first season and after 44, 76, 104, 170, 288 and 412 d in the second season. Seeds were germinated in their fruiting bracts or after bract removal. Bract removal significantly improved seed germination of both shrubs regardless of storage temperature. For S. vermiculata the increase in germination was in the range of 1.3- to 14.7-fold compared with values for the intact fruit in Season 1 and 0.5 to 3.8 in Season 2. Similarly the ranges for A. halimus were 0.5- to 4.2-fold and 0.7- to 5.3-fold in the two seasons respectively. The effect of cold storage was greater on Salsola than on Atriplex. The reduction of the storage temperature from 21°C to 0°C and −22°C increased the longevity of S. vermiculata seeds by 2.8–46.6 times in Season 1 and by 2.9–2.6 times in Season 2. There was little or no effect on the longevity of A. halimus. A leachate prepared by soaking fruiting bracts from S. vermiculata significantly depressed germination (p < 0.01), the effect being greater on Salsola seeds (20% reduction) than on Atriplex seeds (8% reduction). A leachate from A. halimus produced a slight but non-significant reduction in germination.

scholarly journals Conformational Redistribution of Honey Components following Different Storage Conditions

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Giulia Cimo’ ◽  
Pellegrino Conte
Keyword(s):  
Water Distribution ◽  
Room Temperature ◽  
Storage Conditions ◽  
Intramolecular Interactions ◽  
Water Release ◽  
Organic Systems ◽  
Long Term Storage ◽  
Nmr Relaxometry ◽  
Different Temperatures ◽  
Field Cycling

The present study aims at the investigation of the changes in water distribution among the organic components of selected honey samples following honey storage at different temperatures. Results, achieved by application of fast field cycling NMR relaxometry, revealed that the organic constituents were homogeneously distributed within the whole samples stored at room temperature. Conversely, after four months of refrigeration at 4°C, the organic systems were included in persistent clusters, as a consequence of the water release due to the larger stability of the intramolecular interactions over the intermolecular ones. The new conformational arrangements of the honey constituents entailed enhancement of honey moisture content. For this reason, it can be suggested that honey refrigeration prior to storage at room temperature may be detrimental for its long-term storage. In fact, higher risk of fermentation may occur once the sample is warmed after the first refrigeration step.

scholarly journals Different effects of soil drought on soluble carbohydrates of developing Lupinus pilosus and Lupinus luteus embryos

2011 ◽  
Vol 76 (2) ◽  
pp. 119-125 ◽  
Author(s):  
Agnieszka I. Piotrowicz-Cieślak ◽  
Dariusz J. Michalczyk ◽  
Barbara Adomas ◽  
Ryszard J. Górecki
Keyword(s):  
Fold Increase ◽  
Lupinus Luteus ◽  
Soluble Carbohydrates ◽  
Soil Drought ◽  
Soluble Carbohydrate ◽  
Raffinose Family Oligosaccharides ◽  
Normal Soil ◽  
Galactosyl Cyclitols ◽  
Lupinus Pilosus ◽  
Carbohydrate Contents

The aim of this study was to compare the accumulation of soluble carbohydrates in embryos of two lupin species: cultivated <em>Lupinus luteus</em> (cv. Juno) and wild <em>L. pilosus</em>, developing on plants grown under normal soil humidity and soil drought. All analysed seeds accumulated soluble carbohydrates, including: monosaccharides, sucrose, cyclitols, galactosyl cyclitols and raffinose family oligosaccharides. Soil drought caused a nearly two-fold increase of soluble carbohydrate contents in both species. <em>L. pilosus</em> embryos however, responded to water deficiency by increasing the accumulation of cyclitols and galactosyl cyclitols, whereas <em>L. luteus</em> embryos enhanced accumulation of cyclitols and raffinose family oligosaccharides.

scholarly journals Changes in the alkaloid, α-galactoside and protein fractions content during germination of different lupin species

2011 ◽  
Vol 79 (1) ◽  
pp. 11-20 ◽  
Author(s):  
Katarzyna Chilomer ◽  
Karolina Zaleska ◽  
Danuta Ciesiołka ◽  
Piotr Gulewicz ◽  
Andrzej Frankiewicz ◽  
...  
Keyword(s):  
Amino Acids ◽  
Essential Amino Acids ◽  
Antinutritional Factors ◽  
Protein Fractions ◽  
Lupinus Luteus ◽  
Residual Fraction ◽  
Raffinose Family Oligosaccharides ◽  
Germination Process ◽  
Lupin Seeds ◽  
Qualitative Changes

The objective of our studies were seeds of two lupin species <em>Lupinus luteus</em> L. and <em>Lupinus angustifolius</em> L. cvs. Lord and Graf respectively. Lupin seeds were germinated at 15 and 24<sup>o</sup>C and during two, three and four days. In the lupin sprouts antinutritional factors: alkaloids and raffinose family oligosaccharides (RFOs) and five nitrogen fractions: non protein (Nnp), albumin (A), globulin (G), glutelin and prolamin (Gt+P) and nitrogen residue fraction (Nr) were determined. The level of these compounds was compared with the proper ones of initial material (not germinated seeds). These studies showed that the germination process clearly affects the decrease of antinutritional factors: RFOs and alkaloids. The decrease level of these compounds depended on such factors like, lupin species and used germination conditions. It was found on the base of nitrogen analysis of particular protein fractions that the germination process of lupin seeds causes deep quantitative and qualitative changes in fractional composition of lupin proteins. It especially concerns the decrease of globulin and residual fraction content and distinct increase of Nnp fraction. The changes in other fractions were not so unequivocal in comparison with the mentioned above and depended on lupin species, temperature and time of germination. Qualitative changes of A, G and Gt+P fractions caused by germination were confirmed by gel electrophoresis (SDS-PAGE). The amino acid analysis of seeds and sprouts of Nnp fractions showed an increased content of Asp, Ser, Ala, Pro - non essential amino acids (NEAA), and Val, Met, iLeu, Leu, Thr - essential amino acids (EAA). Simultaneously a decrease of Glu, Arg (NEAA), Phe, Lis, Cys (EAA) contents was observed. Generally the germination process causes the decrease of total NEAA and an increase of total EAA in Nnp fractions of both lupin species.

scholarly journals Composition of seed soluble carbohydrates and ultrastructural diversity of testa in lupins from the mediterranean region

2011 ◽  
Vol 74 (4) ◽  
pp. 281-286 ◽  
Author(s):  
Agnieszka I. Piotrowicz-Cieślak
Keyword(s):  
Seed Coat ◽  
Soluble Sugar ◽  
Soluble Sugars ◽  
Lupinus Luteus ◽  
Soluble Carbohydrates ◽  
Embryonic Tissues ◽  
Biochemical Analyses ◽  
Sugar Levels ◽  
Galactosyl Cyclitols ◽  
Lupinus Pilosus

This article present a comparison of soluble sugar levels in seeds of <em>Lupinus atlanticus</em>, <em>Lupinus cosentinii</em>, <em>Lupinus palaestinus</em> and <em>Lupinus pilosus</em>, <em>Lupinus hispanicus</em> subsp. <em>hispanicus</em> and <em>Lupinus luteus</em> of Juno variety. Considering that sugars are accumulated in embryos, only the embryonic tissues were used for biochemical analyses. Additionally, the share of testa and embryo in seed tissues was evaluated. The seed-coat thickness was measured using scanning electron microscopy. The seed coat had the largest share in seeds of <em>Lupinus pilosus</em> and <em>Lupinus palaestinus</em>, and the least share in seeds of <em>Lupinus hispanicus</em> subsp. <em>hispanicus</em> and <em>Lupinus luteus</em> of Juno variety. In the seed of <em>Lupinus pilosus</em> the thickness of the seed coat was 1100 µm, while in <em>Lupinus luteus</em> it was 300 µm. The analysed <em>Lupinus</em> seeds accumulated from 73 mg/g d.m. (dry matter of seed embryo) to 155 mg of soluble sugars/g d.m. The highest quantity of oligosaccharides of the raffinose series was found in seeds of <em>Lupinus luteus</em>, while the lowest amount in seed of <em>Lupinus palaestinus</em>. Galactosyl cyclitols appeared in largest amount in seeds of <em>Lupinus palaestinus</em> and <em>Lupinus pilosus</em>, appropriately 41.93 and 33.75 mg/g dm. The lowest amount of galactosyl cyclitols appeared in <em>Lupinus atlanticus</em>, <em>Lupinus cosentinii</em> and <em>Lupinus hispanicus</em>.

Comparative Labelling and Biokinetic Studies of 99mTc-EDTA(Sn) and 99mTc-DTPA(Sn)

1977 ◽  
Vol 16 (01) ◽  
pp. 30-35 ◽  
Author(s):  
N. Agha ◽  
R. B. R. Persson
Keyword(s):  
Complex Formation ◽  
Significant Influence ◽  
Room Temperature ◽  
Ph Value ◽  
Maximum Activity ◽  
Reduction Step ◽  
Labelling Yield ◽  
Different Temperatures ◽  
Kinetics Of

SummaryGelchromatography column scanning has been used to study the fractions of 99mTc-pertechnetate, 99mTcchelate and reduced hydrolyzed 99mTc in preparations of 99mTc-EDTA(Sn) and 99mTc-DTPA(Sn). The labelling yield of 99mTc-EDTA(Sn) chelate was as high as 90—95% when 100 μmol EDTA · H4 and 0.5 (Amol SnCl2 was incubated with 10 ml 99mTceluate for 30—60 min at room temperature. The study of the influence of the pH-value on the fraction of 99mTc-EDTA shows that pH 2.8—2.9 gave the best labelling yield. In a comparative study of the labelling kinetics of 99mTc-EDTA(Sn) and 99mTc- DTPA(Sn) at different temperatures (7, 22 and 37°C), no significant influence on the reduction step was found. The rate constant for complex formation, however, increased more rapidly with increased temperature for 99mTc-DTPA(Sn). At room temperature only a few minutes was required to achieve a high labelling yield with 99mTc-DTPA(Sn) whereas about 60 min was required for 99mTc-EDTA(Sn). Comparative biokinetic studies in rabbits showed that the maximum activity in kidneys is achieved after 12 min with 99mTc-EDTA(Sn) but already after 6 min with 99mTc-DTPA(Sn). The long-term disappearance of 99mTc-DTPA(Sn) from the kidneys is about five times faster than that for 99mTc-EDTA(Sn).

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