scholarly journals Conformational Redistribution of Honey Components following Different Storage Conditions

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Giulia Cimo’ ◽  
Pellegrino Conte
Keyword(s):  
Water Distribution ◽  
Room Temperature ◽  
Storage Conditions ◽  
Intramolecular Interactions ◽  
Water Release ◽  
Organic Systems ◽  
Long Term Storage ◽  
Nmr Relaxometry ◽  
Different Temperatures ◽  
Field Cycling

The present study aims at the investigation of the changes in water distribution among the organic components of selected honey samples following honey storage at different temperatures. Results, achieved by application of fast field cycling NMR relaxometry, revealed that the organic constituents were homogeneously distributed within the whole samples stored at room temperature. Conversely, after four months of refrigeration at 4°C, the organic systems were included in persistent clusters, as a consequence of the water release due to the larger stability of the intramolecular interactions over the intermolecular ones. The new conformational arrangements of the honey constituents entailed enhancement of honey moisture content. For this reason, it can be suggested that honey refrigeration prior to storage at room temperature may be detrimental for its long-term storage. In fact, higher risk of fermentation may occur once the sample is warmed after the first refrigeration step.

scholarly journals Influence of storage time and temperature on the activity of urease

2019 ◽  
Vol 51 (2) ◽  
pp. 159-163
Author(s):  
B. Alev ◽  
S. Tunali ◽  
R. Yanardag ◽  
A. Yarat
Keyword(s):  
Room Temperature ◽  
Storage Time ◽  
Urease Activity ◽  
Storage Temperature ◽  
Practical Importance ◽  
Storage Conditions ◽  
Relative Activity ◽  
Significant Loss ◽  
Long Term Storage ◽  
Activity Measurements

Enzymes are made of protein, that is why they are sensitive molecules and are affected by storage conditions. A small change in enzyme activity during storage may cause a big error in analysis results. The aim of the study was to evaluate the effects of storage time and temperature on urease activity. Urease solutions were prepared at different activities (from 100 to 2000 U/mL) and stored at room temperature, in the refrigerator (4°C), and in the deep freezer (-18°C and -80°C). Activity measurements were made at regular intervals until 28 days by the modified Weatherburn method. The relative activities of 100-1000 U/mL urease solutions stored at room temperature, 4, -18 or -80°C were 75% and below after 4 days. Twenty-eight days later, for 2000 U/mL urease solutions, only at room temperature, the relative activity was reduced to 37%, while at 4, -18 or -80°C, the relative activities were above 80%. Since urease can be maintained at 4°C for 28 days without significant loss of activity, it has practical importance. Low-activity urease solutions (such as 100-1000 U/mL) should not be stored at -18 or -80°C for short or long term storage, they should be stored at 4°C only for one day. Keywords: Urease activity, storage time, storage temperature

scholarly journals Changes of salivary biomarkers under different storage conditions: effects of temperature and length of storage

2018 ◽  
Vol 29 (1) ◽  
pp. 94-111 ◽  
Author(s):  
Tomás Barranco ◽  
Asta Tvarijonaviciute ◽  
Damián Escribano ◽  
Fernando Tecles ◽  
José J Cerón ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Room Temperature ◽  
Storage Conditions ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Long Term Storage ◽  
Reducing Ability ◽  
Trolox Equivalent ◽  
The Stability ◽  
Term Storage

Introduction: In this report, we aimed to examine the stability of various analytes in saliva under different storage conditions. Materials and methods: Alpha-amylase (AMY), cholinesterase (CHE), lipase (Lip), total esterase (TEA), creatine kinase (CK), aspartate aminotransferase (AST), lactate dehydrogenase (LD), lactate (Lact), adenosine deaminase (ADA), Trolox equivalent antioxidant capacity (TEAC), ferric reducing ability (FRAS), cupric reducing antioxidant capacity (CUPRAC), uric acid (UA), catalase (CAT), advanced oxidation protein products (AOPP) and hydrogen peroxide (H2O2) were colorimetrically measured in saliva obtained by passive drool from 12 healthy voluntary donors at baseline and after 3, 6, 24, 72 hours, 7 and 14 days at room temperature (RT) and 4 ºC, and after 14 days, 1, 3 and 6 months at – 20 ºC and – 80 ºC. Results: At RT, changes appeared at 6 hours for TEA and H2O2; 24 hours for Lip, CK, ADA and CUPRAC; and 72 hours for LD, Lact, FRAS, UA and AOPP. At 4 ºC changes were observed after 6 hours for TEA and H2O2; 24 hours for Lip and CUPRAC; 72 hours for CK; and 7 days for LD, FRAS and UA. At – 20 ºC changes appeared after 14 days for AST, Lip, CK and LD; and 3 months for TEA and H2O2. At – 80 ºC observed changes were after 3 months for TEA and H2O2. Conclusions: In short-term storage, the analytes were more stable at 4 ºC than at room temperature, whereas in long-term storage they were more stable at - 80 ºC than at – 20 ºC.

scholarly journals Directions of Colour Changes of Nectar Honeys Depending on Honey Type and Storage Conditions

2015 ◽  
Vol 59 (2) ◽  
pp. 51-61 ◽  
Author(s):  
Allna Piotraszewska-Pająk ◽  
Anna Gliszczyńska-Świgło
Keyword(s):  
Room Temperature ◽  
Quality Criteria ◽  
Storage Conditions ◽  
Room Temperature Storage ◽  
Long Term Storage ◽  
Colour Parameters ◽  
Colour Changes ◽  
Important Quality ◽  
And Storage ◽  
Temperature Storage

AbstractThe colour of honey is one of the most important quality criteria for consumers. The colour depends mainly on the content of plant pigments but the honey consistency, shape, and size of the crystals may also influence the honey colour parameters. It is related to the crystallisation and decrystallisation processes of honey during storage. In the present study, directions of colour changes of honey during storage were evaluated using a tristimulus colorimeter and the CIE 1976 L*a*b* and CIE L*C*hosystems. The effect of time (3 and 9 months) and storage conditions (cold storage, room temperature storage with access to light, and room temperature storage without access to light) on the colour of nectar honeys was investigated. The results obtained showed that both the type of honey and the storage conditions influenced the honey colour parameters. Significant differences in direction and intensity of the colour changes of honey during storage were observed. These differences make it difficult to indicate which storage conditions are optimal to preserve the colour of the honey. It was found that acacia and heather honeys were the most susceptible to colour changes during long-term storage in all of the study’s applied conditions, whereas rape and buckwheat honeys were the most stable in colour parameters.

scholarly journals Stability of the Combination of Ceftazidime and Cephazolin in Icodextrin or PH Neutral Peritoneal Dialysis Solution

2014 ◽  
Vol 34 (2) ◽  
pp. 212-218 ◽  
Author(s):  
Rahul P. Patel ◽  
Madhur D. Shastri ◽  
Mohammad Bakkari ◽  
Troy Wanandy ◽  
Matthew D. Jose
Keyword(s):  
Peritoneal Dialysis ◽  
Body Temperature ◽  
High Performance ◽  
Room Temperature ◽  
Storage Conditions ◽  
Initial Concentration ◽  
Dialysis Solution ◽  
Different Temperatures ◽  
Colour Changes ◽  
The Stability

IntroductionThe objective of this study was to investigate the stability of ceftazidime and cephazolin in a 7.5% icodextrin or pH neutral peritoneal dialysis (PD) solution.MethodsCeftazidime and cephazolin were injected into either a 7.5% icodextrin or pH neutral PD bag to obtain the concentration of 125 mg/L of each antibiotic. A total of nine 7.5% icodextrin or pH neutral PD bags containing ceftazidime and cephazolin were prepared and stored at 1 of 3 different temperatures: 4°C in a domestic refrigerator; 25°C at room temperature; or 37°C (body temperature) in an incubator. An aliquot was withdrawn immediately before (0 hour) or after 12, 24, 48, 96, 120, 144, 168 and 336 hours of storage. Each sample was analyzed in duplicate for the concentration of ceftazidime and cephazolin using a stability-indicating high-performance liquid chromatography technique. Ceftazidime and cephazolin were considered stable if they retained more than 90% of their initial concentration. Samples were also assessed for pH, colour changes and evidence of precipitation immediately after preparation and on each day of analysis.ResultsCeftazidime and cephazolin in both types of PD solution retained more than 90% of their initial concentration for 168 and 336 hours respectively when stored at 4°C. Both of the antibiotics lost more than 10% of the initial concentration after 24 hours of storage at 25 or 37°C. There was no evidence of precipitation at any time under the tested storage conditions. Change in the pH and color was observed at 25 and 37°C, but not at 4°C.ConclusionPremixed ceftazidime and cephazolin in a 7.5% icodextrin or pH neutral PD solution is stable for at least 168 hours when refrigerated. This allows the preparation of PD bags in advance, avoiding the necessity for daily preparation. Both the antibiotics are stable for at least 24 hours at 25 and 37°C, permitting storage at room temperature and pre-warming of PD bags to body temperature prior to its administration.

Measurement of serum and plasma osmolality in healthy young humans – influence of time and storage conditions

2004 ◽  
Vol 42 (8) ◽  
Author(s):  
Christian C. Seifarth ◽  
Johannes Miertschischk ◽  
Eckhardt G. Hahn ◽  
Johannes Hensen
Keyword(s):  
Room Temperature ◽  
Freezing Point ◽  
Plasma Osmolality ◽  
Storage Conditions ◽  
Plasma Samples ◽  
Freezing And Thawing ◽  
Serum Samples ◽  
Slow Increase ◽  
Stabilizing Agent ◽  
Different Temperatures

AbstractBackground: The precise measurement of osmolality is crucial in the differential diagnosis of disorders of water balance. Storage conditions, and freezing and thawing of serum or plasma samples before osmometry may influence the accuracy of measured values. Methods: A series of serum and plasma samples of 25 healthy young individuals were stored under different conditions at different temperatures (room temperature (22°C), 7°C, –21°C, –78°C) for up to 56 days. Before freezing a protein-stabilizing agent (bacitracin) was added to one part of the samples. Osmolality was examined using the freezing point method. Results: At room temperature osmolality was stable for up to 3 days but showed a tendency toward an increase that was significant on day 14. In contrast, at 7°C an initial significant decrease in serum osmolality occurred (day 1), which was followed by a slow increase. Serum samples stored at –21°C showed a significantly lower osmolality on the 14th day compared to baseline. Adding bacitracin before freezing reduced this decrease by more than half, but the deviation was still significant. In samples stored at –78°C no significant alteration of osmolality from baseline was observed over the observation period of 56 days if samples were thawed in a 37°C water bath. Conclusion: Immediate measurement of osmolality is most reliable in order to obtain accurate values, although storing at room temperature does not influence osmolality significantly during the first 3 days. If storage is necessary for longer, samples should be stored at –78°C and must be thawed quickly (at 37°C). Under these conditions reliable values can be obtained from frozen serum or plasma. Storage at 7°C is not recommended. If samples are stored at –21°C the addition of a protein-stabilizing agent may be useful.

scholarly journals Jet-cooked papermaking starches studied using 1H NMR-relaxometry and viscometry

2020 ◽  
Vol 35 (3) ◽  
pp. 376-385
Author(s):  
Jenna Raunio ◽  
Ekaterina Nikolskaya ◽  
Yrjö Hiltunen
Keyword(s):  
Room Temperature ◽  
Relaxation Rates ◽  
H Nmr ◽  
Nmr Relaxometry ◽  
Surface Sizing ◽  
Cooking Temperature ◽  
Degree Of Oxidation ◽  
Different Temperatures ◽  
Retrograded Starch ◽  
Solids Content

AbstractTwo wet-end starches (potato and barley), one surface sizing starch (barley) and one coating binder starch (barley) were jet-cooked. Samples were collected and stored at 90, 60 and 40 °C. 1H NMR-relaxometry and viscometry were used to monitor the jet-cooked solutions as they cooled to room temperature. Samples stored at different temperatures were also monitored using 1H NMR-relaxometry and viscometry. A sediment formed into the surface sizing and coating binder starches stored at 90 °C. The sediment and supernatant were separated and collected, and measured using 1H NMR-relaxometry. The {T_{2}} relaxation rates of jet-cooked starches showed significant differences between potato and barley starches, as had also been examined in previous studies. The NMR method was also sensitive to differences in solids content and chemical modification (degree of cationization, degree of oxidation and molecular weight). The cooking temperature, cooking speed and viscosity did not influence {T_{2}} relaxation rates. The sediment separated from the surface sizing and coating binder starches held at 90 °C had a significantly higher relaxation rate than the supernatant, indicating that the sediment contained a high amount of retrograded starch.

scholarly journals Physiological-biochemical parameters and characteristics of seed coat structure in lupin seeds subjected to long storage at different temperatures

2011 ◽  
Vol 77 (3) ◽  
pp. 201-205 ◽  
Author(s):  
Agnieszka I. Piotrowicz-Cieślak ◽  
Dariusz J. Michalczyk ◽  
Kamila Górska ◽  
Zofia Bulińska-Radomska ◽  
Ryszard J. Górecki
Keyword(s):  
Seed Coat ◽  
Room Temperature ◽  
Seed Storage ◽  
Lupinus Luteus ◽  
Soluble Carbohydrates ◽  
Raffinose Family Oligosaccharides ◽  
Seed Vigour ◽  
Long Term Storage ◽  
Different Temperatures ◽  
Lupin Seeds

Seed vigour, viability, the contents of soluble carbohydrates, total protein, albumins, and globulins, as well as seed coat structure, were analysed in yellow lupin (<em>Lupinus luteus</em> L.) cv. Iryd seeds stored for 20 years at -14<sup>o</sup>C, 0<sup>o</sup>C or at room temperature (approx. +20<sup>o</sup>C). Seed storage at room temperature reduced viability (to 2%) and increased seed leachate electroconductivity. Determinations of total proteins showed that protein content was significantly reduced in seeds stored at +20<sup>o</sup>C compared to the other storage regimens. Raffinose family oligosaccharides were the main soluble carbohydrates in seeds stored at 0<sup>o</sup>C and -14<sup>o</sup>C, whereas sucrose dominated in seeds stored at room temperature. Scanning electron microscopy (SEM) of seed surface and seed coat sections revealed appearance of an amorphic layer on the surface of seeds stored at room temperature (not observed in other seeds) and distinct shrinking of macrosclereid layer in seeds stored at -14<sup>o</sup>C. Macrosclereids layer in all seeds was 100 um thick and accounted for 60% of seed coat thickness. The obtained results suggest that for long term storage of lupin seeds at 0<sup>o</sup>C is the most advisable temperature if both costs of storage and seed storability are considered.

Efficacy of the Antigenicity-Retaining Ability of Fixative Solutions for Liquid-Based Cytology: Immunocytochemistry of Long-Term Storage

2021 ◽  
pp. 1-12
Author(s):  
Hiaki Sato ◽  
Yoshiaki Norimatsu ◽  
Satoshi Irino ◽  
Takeshi Nishikawa
Keyword(s):  
Cell Membrane ◽  
Room Temperature ◽  
Storage Temperature ◽  
Storage Period ◽  
Storage Conditions ◽  
Immunocytochemical Staining ◽  
Long Term Storage ◽  
Liquid Based Cytology ◽  
Term Storage

<b><i>Introduction/Objective:</i></b> Liquid-based cytology (LBC) is advantageous as multiple stained specimens can be prepared and used for additional assays such as immunocytochemical and molecular-pathological investigations. Two types of preservative-fixative solutions (fixatives) are used for nongynecologic specimens used in the BD SurePath-LBC (SP-LBC) method, and their components vary. However, few studies have evaluated the differences in antigen-retaining ability between these fixatives. Therefore, we investigated and compared the antigen-retaining ability of the fixatives in immunocytochemical staining (ICC) under long-term storage conditions. <b><i>Materials and Methods:</i></b> Sediments of cultured RAJI cells (derived from Burkitt’s lymphoma) were added to each fixative (red and blue) and stored at room temperature for a specified period (1 h; 1 week; and 1, 3, and 6 months). The specimens were then prepared using the SP-LBC method and subjected to ICC. Positivity rate was calculated using the specimens fixed at room temperature for 1 h as a control. Antibodies against Ki67 expressed in the nucleus and against CD20 and leukocyte common antigen (LCA) expressed on the cell membrane were used. <b><i>Results:</i></b> For CD20 and LCA, the positivity rate increased with time in the red fixative compared with that in the control. In the blue fixative, the positivity rate was highest at 1 h and was maintained at a high level throughout the storage period. In contrast, the Ki67 positivity rate was highest at 1 h in both red and blue fixatives and markedly decreased with time. Therefore, although refrigerated (8°C) storage was used, no improvement was noted. <b><i>Conclusions:</i></b> Long-term storage is possible for cell membrane antigens at room temperature; however, it is unsuitable for intranuclear antigens. Therefore, we conclude that suitable fixative type and storage temperature differ based on antigen location. Further investigation is warranted.

EFFECTS OF STORAGE CONDITIONS AND PRESENCE OF FRUITING BRACTS ON THE GERMINATION OF ATRIPLEX HALIMUS AND SALSOLA VERMICULATA

1997 ◽  
Vol 33 (2) ◽  
pp. 149-155 ◽  
Author(s):  
A. E. OSMAN ◽  
F. GHASSALI
Keyword(s):  
Seed Germination ◽  
Cold Storage ◽  
North Africa ◽  
Room Temperature ◽  
Storage Temperature ◽  
Seed Storage ◽  
Storage Conditions ◽  
Shrub Species ◽  
Atriplex Halimus ◽  
Different Temperatures

Two shrub species, Atriplex halimus L. and Salsola vermiculata L., are considered useful for rehabilitation of degraded rangelands in west Asia and north Africa. They can be established from direct seeding and are capable of self-sowing. In this study, seed storage at different temperatures and the influence of fruiting bracts on seed germination were examined for the two species during two seasons. Fruits (utricles) were stored at 20–22°C (room temperature), 0°C or −22°C. Germination tests were carried out after 33, 56, 90, 152, 272 and 397 d in storage in the first season and after 44, 76, 104, 170, 288 and 412 d in the second season. Seeds were germinated in their fruiting bracts or after bract removal. Bract removal significantly improved seed germination of both shrubs regardless of storage temperature. For S. vermiculata the increase in germination was in the range of 1.3- to 14.7-fold compared with values for the intact fruit in Season 1 and 0.5 to 3.8 in Season 2. Similarly the ranges for A. halimus were 0.5- to 4.2-fold and 0.7- to 5.3-fold in the two seasons respectively. The effect of cold storage was greater on Salsola than on Atriplex. The reduction of the storage temperature from 21°C to 0°C and −22°C increased the longevity of S. vermiculata seeds by 2.8–46.6 times in Season 1 and by 2.9–2.6 times in Season 2. There was little or no effect on the longevity of A. halimus. A leachate prepared by soaking fruiting bracts from S. vermiculata significantly depressed germination (p < 0.01), the effect being greater on Salsola seeds (20% reduction) than on Atriplex seeds (8% reduction). A leachate from A. halimus produced a slight but non-significant reduction in germination.

Stability Study of Epirubicin in Nacl 0.9% Injection

1997 ◽  
Vol 31 (9) ◽  
pp. 992-995 ◽  
Author(s):  
Montserrat Pujol ◽  
Montserrat Muñoz ◽  
Josefina Prat ◽  
Victoria Girona ◽  
Jordi De Bolós
Keyword(s):  
Shelf Life ◽  
Room Temperature ◽  
Degradation Kinetics ◽  
Storage Conditions ◽  
Hplc Method ◽  
Stability Study ◽  
Chemistry Laboratory ◽  
Maximum Decrease ◽  
Different Temperatures ◽  
The Stability

Objective To determine the stability of epirubicin in NaCl 0.9% injection under hospital storage conditions. Methods NaCl 0.9% solution was added to epirubicin iyophilized powder to make a final concentration of 1 mg/mL to study the degradation kinetics and 2 mg/mL to study the stability in polypropylene syringes under hospital conditions. Setting Physical chemistry laboratory, Unitat de Fisicoquímica, Universitat de Barcelona. Main outcome Measures Solutions of epirubicin at 2 mg/mL in NaCl 0.9% solutions stored in plastic syringes were studied under hospital conditions at room temperature (25 ± 1 °C) and under refrigeration (4 ± 1 °C) both protected from light and exposed to room light (~50 lumens/m2). All samples were studied in triplicate and epirubicin concentrations were obtained periodically throughout each storage/time condition via a specific stability-indicating HPLC method. To determine the degradation kinetics, solutions of epirubicin in NaCl 0.9% at 1 mg/mL were stored at different temperatures (40, 50, and 60 °C) to obtain the rate degradation constant and the shelf life at room temperature and under refrigeration. Results The degradation of epirubicin in NaCl 0.9% solutions follows first-order kinetics. The shelf life was defined as the time by which the epirubicin concentration had decreased by 10% from the initial concentration. In this study, epirubicin was stable in NaCl 0.9% injection stored in polypropylene containers for all time periods and all conditions. That results in a shelf life of at least 14 and 180 days at 25 and 4 °C, respectively. The maximum decrease in epirubicin concentration observed at 25 °C and 14 days was 4%, and at 4 °C and 180 days was 8%. The predicted shelf life obtained from the Arrhenius equation was 72.9 ± 0.2 and 3070 ± 15 days at 25 and 4 °C, respectively, in both dark and illuminated conditions. Conclusions Solutions of epirubicin in NaCl 0.9% at 2 mg/mL are chemically stable when they are stored in polypropylene syringes under hospital storage conditions. No special precaution is neccessary to protect epirubicin solutions (2 mg/mL) from light.

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