scholarly journals Liquid shoot culture of Salvia officinalis L. for micropropagation and production of antioxidant compounds; effect of triacontanol

2011 ◽  
Vol 77 (2) ◽  
pp. 99-104 ◽  
Author(s):  
Izabela Grzegorczyk ◽  
Halina Wysokińska
Keyword(s):  
Liquid Medium ◽  
Rosmarinic Acid ◽  
Shoot Culture ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Herbal Product ◽  
Dry Weight ◽  
Salvia Officinalis ◽  
Carnosic Acid ◽  
Antioxidant Compounds

Liquid shoot culture of <em>Salvia officinalis</em> L. in MS medium containing IAA (0.1 mg l<sup>-1</sup>) and BAP (0.45 mg l<sup>-1</sup>) was developed and evaluated in relation to shoot multiplication and antioxidant compound (carnosic acid, carnosol and rosmarinic acid) accumulation. In the liquid medium, on average, 3 new shoots per explant (shoot tip) were obtained within 3 weeks. The shoots produced 8.2±0.02 mg of diterpenoids and 31.2±0.29 mg of rosmarinic acid per gram of dry weight. Shoot proliferation and diterpenoid content increased when triacontanol (5, 10 or 20 pg l<sup>-1</sup>) was added to the liquid medium. In optimum conditions (at 20 pg l<sup>-1</sup> TRIA) almost 7 shoots were formed per explant after 3 weeks. An increase in diterpenoid production (expressed as the sum of carnosol and carnosic acid) ranged from 30% to 50% and dependended on triacontanol concentration tested. The level of diterpenoids in triacontanol-treated shoots was similar to the content of compounds in commercial herbal product (dried leaves of <em>S. officinalis</em>) (10-12 mg g<sup>-1</sup> dry wt). Triacontanol did not increase rosmarinic acid production, but the content of the phenolic as compound in shoots grown in liquid culture (31 mg g<sup>-1</sup> dry wt) was even 24 times higher compared to samples of dried leaves of <em>S. officinalis</em> plants. We also demonstrated that the highest amounts of CA, Car and RA were accumulated in young, top parts of sage shoots. This observation could be useful for improving the selection of material for the extraction of natural antioxidants from <em>S. officinalis</em>.

scholarly journals The effect of triacontanol on shoot multiplication and production of antioxidant compounds in shoot cultures of Salvia officinalis L.

2011 ◽  
Vol 75 (1) ◽  
pp. 11-15 ◽  
Author(s):  
Izabela Grzegorczyk ◽  
Ireneusz Bilichowski ◽  
Elżbieta Mikiciuk-Olasik ◽  
Halina Wysokińska
Keyword(s):  
Rosmarinic Acid ◽  
Hplc Analysis ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Carnosic Acid ◽  
Antioxidant Compounds ◽  
Shoot Cultures ◽  
Methanolic Extracts ◽  
Salvia Officinalis L

This report describes the effect of triacontanol on shoot multiplication and production of antioxidant compounds (carnosic acid, carnosol and rosmarinic acid) in <em>S. officinalis</em> cultures grown on MS basal medium (agar solidified medium supplemented with 0.1 mg l<sup>-1</sup> IAA, 0.45 mg l<sup>-1</sup> BAP). It was found that shoot proliferation significantly increased when triacontanol at concentrations of 5, 10 or 20 µg l<sup>-1</sup> was added to the medium. HPLC analysis of acetone and methanolic extracts of sage shoots showed that the production of diterpenoids, carnosic acid/carnosol ratio, as well as, contents of rosmarinic acid were also affected by the treatment with triacontanol. The highest stimulation effect of triacontanol was observed on the production of carnosol, where the treatment with 20 µg l l<sup>-1</sup> increased the content of this diterpenoid 4.5-fold compared to that in the control (sage shoots growing on MS basal medium, only).

scholarly journals In vitro cultures of Salvia officinalis L. as a source of antioxidant compounds

2011 ◽  
Vol 74 (1) ◽  
pp. 17-21 ◽  
Author(s):  
Izabela Grzegorczyk ◽  
Ireneusz Bilichowski ◽  
Elżbieta Mikiciuk-Olasik ◽  
Halina Wysokińska
Keyword(s):  
Cell Suspension ◽  
Rosmarinic Acid ◽  
Dry Weight ◽  
Suspension Cultures ◽  
In Vitro Cultures ◽  
Salvia Officinalis ◽  
Carnosic Acid ◽  
Shoot Cultures ◽  
Undifferentiated Callus

The concentrations of carnosic acid, carnosol and rosmarinic acid in different materials from differentiated (multiple shoot cultures and regenerated plants) and undifferentiated (callus and cell suspension) in vitro cultures of <em>Salvia officinalis</em> were determined by HPLC. The results suggested that diterpenoid (carnosic acid and carnosol) production is closely related to shoot differentiation. The highest diterpenoid yield (11.4 mg g<sup>-1</sup> for carnosic acid and 1.1 mg g<sup>-1</sup> for carnosol) was achieved in shoots of 10-week-old micropropagated plants. The levels were comparable to those found in shoots of naturally growing plants. Undifferentiated callus and cell suspension cultures produced only very low amounts of carnosol (ca. 0.05 mg g<sup>-1</sup> of dry weight). In contrast, content of rosmarinic acid in callus and suspension cultures as well as shoots growing in vitro and in vivo was similar and ranged between 11.2 and 18.6 mg g<sup>-1</sup> of dry weight.

scholarly journals Antioxidant compounds in Salvia officinalis L. shoot and hairy root cultures in the nutrient sprinkle bioreactor

2011 ◽  
Vol 79 (1) ◽  
pp. 7-10 ◽  
Author(s):  
Izabela Grzegorczyk ◽  
Halina Wysokińska
Keyword(s):  
Hairy Root ◽  
Shoot Culture ◽  
Growth Period ◽  
Biomass Accumulation ◽  
Fold Increase ◽  
Operating Conditions ◽  
Salvia Officinalis ◽  
Carnosic Acid ◽  
Antioxidant Compounds ◽  
Shoot Cultures

The study focused on the production of compounds with antioxidant activity in hairy root and shoot cultures of <em>Salvia officinalis</em> grown in laboratory-scale sprinkle nutrient bioreactors. HPLC analysis showed that production of rosmarinic acid in transformed roots (34.65 ±1.07 mg l<sup>-1</sup>) was higher that in shoot culture (26.24 ±0.48 mg l<sup>-1</sup>). In the latter diterpenoids: carnosic acid (1.74 ±0.02 mg l<sup>-1</sup>) and carnosol (1.34 ±0.01 mg l<sup>-1</sup>) were also found. Biomass accumulation after a growth period in the bioreactor was also studied. An 18-fold increase in hairy root biomass was recorded after 40 days of culture. In sage shoot culture, biomass increased 43 times after 21 days of bioreactor run. The current operating conditions of the bioreactor were not suitable for the propagation of <em>Salvia officinalis</em> mainly due to the hyperhydricity problem of leaves and stems.

Influence of sodium salicylate on rosmarinic acid, carnosol and carnosic acid accumulation by Salvia officinalis L. shoots grown in vitro

2015 ◽  
Vol 37 (8) ◽  
pp. 1693-1701 ◽  
Author(s):  
Margareta Kračun-Kolarević ◽  
Slavica Dmitrović ◽  
Biljana Filipović ◽  
Marija Perić ◽  
Danijela Mišić ◽  
...  
Keyword(s):  
Rosmarinic Acid ◽  
Sodium Salicylate ◽  
Salvia Officinalis ◽  
Carnosic Acid ◽  
Acid Accumulation ◽  
Salvia Officinalis L

scholarly journals Proliferation-rate Effects of BAP and Kinetin on Banana (Musa spp. AAA Group) ‘Basrai’

HortScience ◽  
2007 ◽  
Vol 42 (5) ◽  
pp. 1253-1255 ◽  
Author(s):  
Aish Muhammad ◽  
Hamid Rashid ◽  
Iqbal Hussain ◽  
S.M. Saqlan Naqvi
Keyword(s):  
Liquid Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Multiplication Rate ◽  
Ms Medium ◽  
Shoot Height ◽  
Significant Difference ◽  
Rate Effects ◽  
Single Shoot ◽  
Number Of Shoots

The effect of benzylaminopurine (BAP) and kinetin alone and in combination with indole 3-acetic acid (IAA) on shoot proliferation of ‘Basrai’ (Musa spp., AAA group) was investigated. Shoot tips (4–6 mm) were excised from field-grown suckers to initiate the cultures. Concentrations of BAP and kinetin ranged from 0.0 to 8.0 mg·L−1 each on solid or in liquid MS medium. When liquid medium was used, cultures were agitated continuously on an orbital shaker moving at 40 rpm. Three subculture regimes were employed; after each subculture, the number of shoots regenerated from each explant was counted. The results showed that the multiplication rate was significantly (P ≤ 0.05) dependent upon cytokinin type, its concentration, and type of medium used. The maximum number of shoots regenerated from a single shoot tip was achieved in liquid MS medium containing 4.0 mg·L−1 BAP. There was no significant difference between liquid and solid medium when kinetin was used; however, kinetin at 4.0 mg·L−1 or above yielded significant results as compared with the control and lower kinetin concentrations. The results demonstrated that 4.0 mg·L−1 BAP with 1.0 mg·L−1 IAA in liquid medium was best for shoot multiplication and shoot height during micropropagation of ‘Basrai’.

scholarly journals Supercritical Antisolvent Fractionation of Antioxidant Compounds from Salvia officinalis

2021 ◽  
Vol 22 (17) ◽  
pp. 9351
Author(s):  
Raquel Mur ◽  
Juan I. Pardo ◽  
M. Rosa Pino-Otín ◽  
José S. Urieta ◽  
Ana M. Mainar
Keyword(s):  
Antioxidant Activity ◽  
Rosmarinic Acid ◽  
Supercritical Co2 ◽  
Qsar Model ◽  
Salvia Officinalis ◽  
Training Dataset ◽  
Antioxidant Compounds ◽  
Supercritical Antisolvent ◽  
Pure Compounds ◽  
Co2 Flow

The increasing interest towards greener antioxidants obtained via natural sources and more sustainable processes encourages the development of new theoretical and experimental methods in the field of those compounds. Two advanced separation methods using supercritical CO2 are applied to obtain valuable antioxidants from Salvia officinalis, and a first approximation to a QSAR model relating molecular structure with antioxidant activity is explored in order to be used, in the future, as a guide for the preselection of compounds of interest in these processes. Separation experiments through antisolvent fractionation with supercritical CO2 were designed using a Response Surface Methodology to study the effect of pressure and CO2 flow rate on both mass yields and capability to obtain fractions enriched in three antioxidant compounds: chlorogenic acid, caffeic acid and rosmarinic acid which were tracked using HPLC PDA. Rosmarinic acid was completely retained in the precipitation vessel while chlorogenic and caffeic acids, though distributed between the two separated fractions, had a major presence in the precipitation vessel too. The conditions predicted for an optimal overall yield and enrichment were 148 bar and 10 g/min. Although a training dataset including much more compounds than those now considered can be recommended, descriptors calculated from the σ-profiles provided by COSMO-RS model seem to be adequate for estimating the antioxidant activity of pure compounds through QSAR.

scholarly journals Application of UHPLC-ESI-QTOF-MS in Phytochemical Profiling of Sage (Salvia officinalis) and Rosemary (Rosmarinus officinalis)

2020 ◽  
Vol 07 (04) ◽  
pp. e133-e144
Author(s):  
Ravikishore Velamuri ◽  
Yashaswini Sharma ◽  
John Fagan ◽  
Jim Schaefer
Keyword(s):  
Rosmarinic Acid ◽  
Antioxidant Properties ◽  
Polyphenolic Compounds ◽  
Rosmarinus Officinalis ◽  
Salvia Officinalis ◽  
Carnosic Acid ◽  
Flavonoid Compound ◽  
Pentacyclic Triterpenoid ◽  
Corosolic Acid ◽  
First Time

AbstractUHPLC with QTOF-MS is widely used as a powerful tool for metabolomic analysis. This technology has recently been applied to the analysis of polyphenols in food and herb extracts. Sage (Salvia officinalis) and rosemary (Rosmarinus officinalis), belonging to the family Lamiaceae, are known for their potent antioxidant properties due to the presence of polyphenols. We have developed a sensitive and reproducible UHPLC-QTOF-MS/MS-based method for comprehensive phytochemical profiling and the identification and quantitation of specific polyphenolic compounds present in sage and rosemary leaves. The herbs were extracted ultrasonically using methanol as the solvent. In sage, rosmarinic acid (17 678.7±673.4 µg/g) and 12-methoxy carnosic acid (21 918.3±715.4 µg/g) were found in the highest concentrations among all polyphenols. In contrast, rosmarinic acid (14 311.0±636.4 µg/g), luteolin-3'-acetyl-O-glucuronide (1488.50±47.58 µg/g), and luteolin-7-O-glucuronide (1053.68±68.83 µg/g) were observed in the highest concentrations in rosemary. Sagerinic acid, rosmanol, rosmadial, carnosol, and carnosic acid were found in abundance in both sage and rosemary. The pentacyclic triterpenoid, corosolic acid ([M - H]¯ m/z 471.35), was detected for the first time in both plants. Of the 47 polyphenolic compounds identified in each plant, 38 compounds were found in common in rosemary and sage. A flavonoid compound, baicalin ([M -H]¯ m/z 445.08), was identified for the first time in S. officinalis. Also, pectolinarigenin ([M - H]¯ m/z 313.07), a dimethoxyflavone, was detected for the first time in both sage and rosemary leaves.

Establishment of Salvia officinalis L. Hairy Root Cultures for the Production of Rosmarinic Acid

2006 ◽  
Vol 61 (5-6) ◽  
pp. 351-356 ◽  
Author(s):  
Izabela Grzegorczyk ◽  
Aleksandra Królicka ◽  
Halina Wysokińska
Keyword(s):  
Hairy Root ◽  
Hairy Roots ◽  
Rosmarinic Acid ◽  
Root Culture ◽  
Dry Weight ◽  
Salvia Officinalis ◽  
Root Cultures ◽  
Hairy Root Line ◽  
Salvia Officinalis L ◽  
Agrobacterium Rhizogenes Strains

Shoots of Salvia officinalis, a medicinally important plant, were infected with Agrobacterium rhizogenes strains ATCC 15834 and A4 which led to the induction of hairy roots in 57% and 37% of the explants, respectively. Seven lines of hairy roots were established in WP liquid medium under light and dark conditions. The transformed nature of the root lines was confirmed by polymerase chain reaction using rolB and rolC specific primers. Transformed root cultures of Salvia officinalis showed variations in biomass and rosmarinic acid production depending on the bacterial strain used for transformation and the root line analyzed. Both parameters (growth and rosmarinic acid content) of ATCC 15834-induced lines were significantly higher than the A4-induced lines. The maximum accumulation of rosmarinic acid (about 45 mg g-1 of dry weight) was achieved by hairy root line 1 (HR-1) at the end of the culture period (45D50 days). The level was significantly higher than that found in untransformed root culture (19 mg g-1 of dry wt).

scholarly journals Extraction of Carnosic Acid and Carnosol from Sage (Salvia officinalis L.) Leaves by Supercritical Fluid Extraction and Their Antioxidant and Antibacterial Activity

Plants ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 16 ◽  
Author(s):  
Valentina Pavić ◽  
Martina Jakovljević ◽  
Maja Molnar ◽  
Stela Jokić
Keyword(s):  
Supercritical Fluid ◽  
Flow Rate ◽  
Acid Content ◽  
Antioxidant Activities ◽  
Salvia Officinalis ◽  
Carnosic Acid ◽  
Antioxidant Compounds ◽  
Bacterial Strains ◽  
Salvia Officinalis L ◽  
Co2 Flow

Sage (Salvia officinalis L.) is a good source of antioxidant compounds, carnosic acid and carnosol being the prominent ones. Both are soluble in CO2, and our goal was to investigate the application of supercritical CO2 extraction to obtain sage extracts rich in these compounds. The effect of pressure, temperature, and CO2 flow rate on the carnosic acid and carnosol yield was studied. These variables were optimized by response surface methodology (RSM). The pressure significantly affected carnosol extraction, while the extraction of carnosic acid was affected by the pressure, temperature, and CO2 flow rate. Carnosic acid content varied from 0.29–120.0 µg mg−1, and carnosol content from 0.46–65.5 µg mg−1. The optimal conditions according to RSM were a pressure of 29.5 MPa, a temperature of 49.1°C, and a CO2 flow rate of 3 kg h−1, and the sage extract yield was calculated to be 6.54%, carnosic acid content 105 µg mg−1, and carnosol content 56.3 µg mg−1. The antioxidant activities of the sage extracts were evaluated by the scavenging activities of 2,2-diphenyl-1-picrylhydrazyl (DPPH). Sage extract obtained at 30 MPa and 40°C with 2 kg h−1 CO2 flow rate with a carnosic acid content of 72 µg mg−1 and carnosol content of 55 µg mg−1 exhibited the highest antioxidant activity (80.0 ± 0.68%) amongst the investigated supercritical fluid extracts at 25 µg mL−1 concentration. The antimicrobial properties of extracts were tested on four bacterial strains: Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, and Staphylococcus aureus. The extract with a carnosic acid content of 116 µg mg−1 and a carnosol content of 60.6 µg mg−1 was found to be the most potent agent against B. subtilis.

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